LOCUS       V007585                10707 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007585
VERSION     V007585
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Sand A, Holwerda EK, Ruppertsberger NM, Maloney M, Olson DG, Nataf
            Y, Borovok I, Sonenshein AL, Bayer EA, Lamed R, Lynd LR, Shoham Y.
  TITLE     Three cellulosomal xylanase genes in Clostridium thermocellum are
            regulated by both vegetative SigA (sigma(A)) and alternative SigI6
            (sigma(I6)) factors
  JOURNAL   FEBS Lett. 589 (20), 3133-3140 (2015)
   PUBMED   26320414
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Holwerda EK.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2015) Thayer School of Engineering, Dartmouth
            College, 14 Engineering drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10707)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "FEBS
            Lett."; date: "2015"; volume: "589"; issue: "20"; pages: "3133-3140"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JUL-2015) Thayer School of Engineering, Dartmouth College, 14
            Engineering drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..514
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(1202..1960)
                     /gene="sigI6"
                     /label="RNA polymerase sigma factor SigI6"
                     /note="RNA polymerase sigma factor SigI6 from Acetivibrio
                     thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 /
                     NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372).
                     Accession#: A3DH98"
     CDS             2594..3241
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3262..3807
                     /codon_start=1
                     /product="hypoxanthine phosphoribosyltransferase"
                     /EC_number="2.4.2.8"
                     /label="hypoxanthine phosphoribosyltransferase"
                     /protein_id="ALI61571.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     terminator      4703..4950
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5198..5786)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5873..6451)
                     /codon_start=1
                     /product="thymidine kinase"
                     /EC_number="2.7.1.21"
                     /label="thymidine kinase"
                     /protein_id="ALI61572.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     CDS             complement(7151..8152)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             complement(8713..9570)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(9669..10469)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(10470..10685)
                     /label="URA3 promoter"
ORIGIN
        1 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
       61 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
      121 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
      181 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
      241 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
      301 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
      361 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
      421 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
      481 atacgaggcg cgtgtaagtt acaggcaagc gatccgtccg ccggcgaacg tggcgagaaa
      541 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      601 gcgcgtaacc accacacccg ccgcgcttaa tggggcgcta cagggcgcgt ggggatgatc
      661 cactagtaag cttggatcaa acggcaaaaa ggacaggtcc aaaaaaaccg aagtggaaaa
      721 cctcataaag gaatttggtc ttgaaggttt cgaaaacttc tatccggggg aaatatccgg
      781 cggaatgaag caaagagcag gtattcttcg cactttttta atggaaagcg acataatgct
      841 tcttgacgaa ccctttggag cgcttgatgc aataacccgg ataaatatgc aggaatggct
      901 gctgaaagtc ctggttgagc acaaaaagac cgtattgttt ataacccacg acattgaaga
      961 ggcaatcttt ctgtcggaca gggtgtatgt cctgtccggc agacctgccc atgttataaa
     1021 aactttggat atagagtttc cccgaccaag ggacagagaa gtgctttctt ctccaaaatt
     1081 tctggaatac aaagatgctc ttttgaaagc ccttctctga aaagttttat atgaaaagtc
     1141 ttatatgaaa agttttctct aaaaagcttt caagcatcct ttcccgcatt caaaacaatc
     1201 accgcaaatc cacctccttt tcctggatac ctgcagcata ctccttgaag atttcgaggt
     1261 tgctccttaa tatcaagctt actgcaatta tatatttttt atttctttct atagtccttc
     1321 tgctaacttt tgccagtttc aacagttcca atgtgggcaa ctttttggtt ttttttagct
     1381 tttcataaag cccgtcatta cttgcaattt tttttgcaat atttatcaaa agctctctcg
     1441 aatctctgtg ctttggagta caggagagca aatccttaaa agttatatca aaggaggcca
     1501 gctcagattt gaaaagtcta atctcgtccg taaattccaa tctttcaatt gcattgttgc
     1561 cgtcagcatc cgaaagagtt ctttcaagtt tgatatcttc gttttcaaag taagaaaaag
     1621 gataaaccat tttattctta tgattttttc ttttatagtc aataagtctt ctattaataa
     1681 cctgttctga gaaaacaagg aagttagaat gcttctcttc atcataagca ttgatggctt
     1741 cattgaaagc caataatgca acgctgtatt cttcactgtt ttccggctca acatgcctgt
     1801 cagtcgcctt atacaccaat tttaatataa aaggcctaaa cctcaggata aactcttccc
     1861 ttgcagaatc atccccggct tttattctgt tcagatactc tataataatc ctttttgtat
     1921 gttccctgtc gtctcgcgag gccggccagt attctgacat gggtgtatca ataacccatg
     1981 cgtttccgta ttgtatcgga atggtttcgg acagggcggt gggaataggc atggaaaaga
     2041 tttttttgcc cgaggatgca ttgataaagt atttcttttc cgaaagagag gaaaagattc
     2101 taaagagttt tggaaatact gatgaatatt gtgtgcagag tacaattcta tggacaagaa
     2161 aagaggcttt gtcaaaactt tttcgtctgg gaatgaggat ggattttaaa aagctggata
     2221 ctttggaggg cgaggtggtt tttcaggaaa caaacagggc gcgtctgttt tcttttatat
     2281 gcaataatta ctgtatctct ctggcattgc caggttttaa taaagattaa aattattgac
     2341 tagaaataaa aaaattgtcc ataatattaa tggacaaaaa aacaaagaat tacatcaaag
     2401 gaagataaaa atactttgtt aaaaaattaa ttatttttta tctaaactat tgaaaatgaa
     2461 aataaaataa tataaaatga atcatagtgc aagagatact tgccagagga tgaatatttt
     2521 actgcattca tgctttatgg cagctaatag aggcattaaa ttaaatttta atttacaata
     2581 ggaggcgata ttaatgaact ttaataaaat tgatttagac aattggaaga gaaaagagat
     2641 atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa ttgatattag
     2701 tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat ttattttctt
     2761 agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata gcgacggaga
     2821 gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg tatctaaaac
     2881 attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg atttatacct
     2941 ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac ctatacctga
     3001 aaatgctttt tctctttcta ttattccatg gacttcattt actgggttta acttaaatat
     3061 caataataat agtaattacc ttctacccat tattacagca ggaaaattca ttaataaagg
     3121 taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg atggttatca
     3181 tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg actggctttt
     3241 ataataaagg aggtcgacgt catggaaaat ttatcaaaag acatcgatga aattttgatc
     3301 acagaagaag aacttaagga aaagataaaa gagcttggga ggcaaatcac aaaagactac
     3361 aaagggaaaa atttgatgtt ggtaggagtt ttaaaaggtg ctttaatgtt tatggctgat
     3421 ttgtcaagac acatagattt gcctttatca cttgatttta tggctgtttc cagctatgga
     3481 agctcaactc attcatcagg aatagtaaag ataatcaaag atcttgatat aagcatagaa
     3541 ggcaaagatg ttctgattgt ggaagacata attgacagcg gtttgacttt gtcttactta
     3601 agggaaactt tacttggaag gaagccaaaa agcctgaaaa tatgcacaat attagacaaa
     3661 ccggagagaa gagaagcatc tgtaaaagtc gattatgtag gatttaagat acctgataag
     3721 tttgtcgtgg gttatggatt ggactttgat gaaaagtaca ggaaccttcc ttttataggc
     3781 gttttgaaac ctgaaatgta cagctaagcc cgggcctcga gaaaacaaaa ggctcagtcg
     3841 gaagactggg ccttttgttt tggtaccgaa ttcggttccc ggtgtttctg ttttgcccgg
     3901 tgtttcgttc ttggacaaag caggtgtact gtcaaccgaa cctgtcgcgg tgttaccttc
     3961 cgtcctgccg ggtgttgagg tagcaggcaa agtttcagtc acttcaggca cggcactttc
     4021 cgcaggtccg gtatttatcg catccaggtt gtctttatcc tctgttacaa tatcctccgg
     4081 aacattctca ttatcaatgc cgaccttggc aatgagctcc agcaaatccg catctttaat
     4141 ctcatcaata gtcaaattta cattctgctc tttggctttt aaatacacgg catatctgcc
     4201 cattgaaata ttttcttctt tggatgattc tctttcctcc aaagtaagat ttaaaatcct
     4261 ggttgtaata ccgctgtttt taaggtctga aaccgccttg ttcacatcat caaaaagctg
     4321 accgatttta ttttgaacgt ccttattctc cgaaccttcc cctgctttta aatcacaggc
     4381 tgtggatata aggattatat tctccttctc cctggaaata aatccaagct ccatgctctt
     4441 ttgcacaatc tcattcacag cctccgccac tgttttttct tttaattcaa gtccttccag
     4501 cactgtctcg gcatcgccgt tcagtgcttt ggcttcggct attttgttgt tgtaatttat
     4561 ccaaagctca atacttggat ttatatcaat gccaacataa gcatatatat tttttcttgc
     4621 cgtatttcca ttaataaaca tcagcacaaa agacaacaca acgcgcctca gcgtttaaac
     4681 ccgctgatcc tagagggccg catcatgtaa ttagttatgt cacgcttaca ttcacgccct
     4741 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     4801 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     4861 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     4921 gttttgggac gctcgaaggc tttaatttgc aagctgcggc cctgcattaa tgaatcggcc
     4981 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5041 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5101 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5161 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5221 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5401 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5521 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5581 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5641 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5701 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5821 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaaaaaatcc gcttaagtcc
     5881 gcgggacttc atggcacttt ctacaccttg cttcataaga ctcctttgct ccaaccatta
     5941 taatgggatc gtcgtaattg gcaggcttcc catttataag cctttgcgtg cgagtagcag
     6001 gattgccaca cttcatgcat atagcagtaa gcttatcgac aaattcagct atggccatca
     6061 attctggagt cggaccaaat ggttcacctc taaagtccat gtcaagccct gcacatatga
     6121 ctcttttccc gctatcggca atctctttta cgatgtcgac tatttcagaa tcaaaaaatt
     6181 gaacttcatc tatagcaaat acatccgtat cttcttcagc ataagccaat atgtcagaag
     6241 cctttactat ggcaatggcg tgcatgttgt cgccgttatg agatacgact ttgtctatgg
     6301 aatacctatc gtctatagcc ggtttgaaaa cctgaacttt ttgcctggca atcttagctc
     6361 tctttatcct tcttataagt tcctcacttt ttccactgaa catgggacca gttacaactt
     6421 ctatgtaccc gtggtcttta ggcccataca tctttcgtcc tccttaaaat tttcgtttat
     6481 ttgtttttta tttagtgtgc ctaaaagcac catcttatac attcgtacct tatatctctt
     6541 atcttgtatc tcttacctca tatctcttat tattaactac cgaagtatat tttcataacg
     6601 gaatcttatg acttattttt ctgactgccg ttttaccata cggttaattt atacggaaac
     6661 agctaatcta accttgctga aggcatgttt accaaaatat tatatctact ttaccatatt
     6721 aattcaagtg gtcattttga tattttacgc caaaattttt agcgttcctt tacttgataa
     6781 tttatccaaa aaccttcatt cgtcaaaaca attatgatat aattaattta tgatatgatt
     6841 aattaatatt tacggataac taatcatttc aatgcaatag atcaacatgc aaatcaatat
     6901 ccaccttact ccgaacattt tgcaccaacg aatgcccagc actatatccc ggcaagcaat
     6961 ataacgtcta taatttttta tgataacgat aaaattaaat tttatgaaag gggtaaaagc
     7021 atgagaaaaa tagctgtatt gttaattact ttgacgttct tagtcacgac tcgaatttag
     7081 gaggcttact tgtctgcttt cttcattaga atcaatcctt ttttaaaagt caatcccgtt
     7141 tgttgaacta ctctttaata aaataatttt tccgttccca attccacatt gcaataatag
     7201 aaaatccatc ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg
     7261 tgtcatcaag gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc
     7321 ttttacggtg taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat
     7381 cggtcataaa atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta
     7441 tatccgattt atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt
     7501 ctaatttcat tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg
     7561 tattcttaaa ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat
     7621 tatctttatt atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa
     7681 tttttttata ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta
     7741 attcttcgcc atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg
     7801 gcttttgttt aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc
     7861 tcctccagtt gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc
     7921 tttcgcctgt ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt
     7981 cagccttttt aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct
     8041 tttctctcca tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt
     8101 catctgaata aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag
     8161 ttatttgttt ggtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa
     8221 gggttttcaa tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta
     8281 aaataaaaat gacgttattt ctatatgtat caagaataga aagaactcgt ttttcgctac
     8341 gctcaaaacg caaaaaaagc actcattcga gtgctttttc ttatcgctcc aaatcatgcg
     8401 attttttcct ctttgctttt ctttgctcac gaagttctcg atcacgctgc aaaacatctt
     8461 gaagcgaaaa agtattcttc ttttcttccg atcgctcatg ctgacgcacg aaaagccctc
     8521 taggcgcata ggaacaactc ctaaatgcat gtgaggggtt ttctcgtcca tgtgaacagt
     8581 cgcatacgca atattttgtt tcccatagcg gccgcgatta tcaaaaagga tcttcaccta
     8641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agcgcttacc
     8821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatggg taataactga
     9601 tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca
     9661 gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa
     9721 cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata
     9781 atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct
     9841 cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt
     9901 ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg
     9961 tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct
    10021 aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta
    10081 acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg
    10141 tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct
    10201 tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc
    10261 atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat
    10321 gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt
    10381 gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca
    10441 gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt
    10501 ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt
    10561 atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc
    10621 gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa
    10681 ttgaattgaa aagctagctt atcgatg
//
