LOCUS       62056_9130        8567 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1763..1783
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             1805..1825
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             2073..3722
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3994..4049
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        complement(4407..4425)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        4771..4875
                     /label=AmpR promoter
     CDS             4876..5733
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5907..6495
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6753..6771
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6809..7306
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7325..7921
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        8565..8567
                     /label=CAG
                     /note="CMV early enhancer fused to modified chicken 
                     beta-actin promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gsgcgcgccr gggggggggg gggggggggg gggggggggs
      541 ggggsgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattaat tcagatccgc
     1741 cgccactatg ggatcaagat cgccaaaaaa gaagagaaag gtgccgaaga agcatgcagc
     1801 accaccaaaa aaaaaacgaa aagtagaaga cccacgaggc aacaccagcg gcgtgctgag
     1861 cacccccaag gccaagaggg ccaagcaccc ccccggcacc gagaagccca ggagcaggag
     1921 ccagagcgag cagcccgcca cctgccccat ctgctacgcc gtgatcaggc agagcaggaa
     1981 cctgaggagg cacctggagc tgaggcactt cgccaagccc gcctagggat aacagggtaa
     2041 ttagatgaca agcccggcgt ggatccgcca ccatggaaga cgccaaaaac ataaagaaag
     2101 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta
     2161 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg
     2221 acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
     2281 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
     2341 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
     2401 atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca
     2461 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta
     2521 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
     2581 ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca
     2641 agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc
     2701 tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc
     2761 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
     2821 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
     2881 aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa
     2941 ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
     3001 acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga
     3061 ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc
     3121 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
     3181 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac
     3241 tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
     3301 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
     3361 aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg
     3421 ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg
     3481 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
     3541 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
     3601 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
     3661 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg
     3721 tgtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     3781 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3841 gttgttaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     3901 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     3961 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     4021 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     4081 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     4141 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     4201 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     4261 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     4321 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     4381 acctgcagcc caagctggcg tcgagttcta tagtgtcacc taaatcgtat gtgtatgata
     4441 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtccat atggtgcact
     4501 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4561 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4621 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4681 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4741 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5101 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6661 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6721 cattaatgca ggttaacctg gcttatcgaa attaatacga ctcactatag ggagaccggc
     6781 agatctgata tcatcgatga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6841 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6901 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     6961 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     7021 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     7081 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     7141 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     7201 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     7261 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgaccg atccagccgc
     7321 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     7381 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     7441 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7501 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7561 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7621 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7681 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7741 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7801 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7861 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7921 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     7981 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgcg
     8041 ggggacacac cgaacacgcc gaccctgctg aacacgcggc gcagttcggt gcccaggagc
     8101 ggatcgaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct
     8161 gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga
     8221 gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt
     8281 tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca
     8341 aattaagggc cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg
     8401 atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg
     8461 tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc
     8521 agtattgttt tgccaagttc taattccatc agaagcttca gctgctc
//
