LOCUS       62056_9120        3990 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    510..531
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        546..576
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..600
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     608..624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             716..738
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             752..769
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             1184..1210
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1211..1234
                     /codon_start=1
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
                     /translation="LEVLFQGP"
     primer_bind     complement(1998..2014)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2488..2592
                     /label=AmpR promoter
     CDS             2593..3450
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3624..3990
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      601 tttcacacag gaaacagcta tgacatgatt acgaatttag atctgaattc gggcccttcg
      661 aaaattaata cgactcacta tagggagacc acaacggttt ccctctagaa ataattttgt
      721 ttaactttaa gaaggagata tattcatggc tcatcaccat caccatcacg cggcccagcc
      781 ggccatggct caggtgcagc tggtggagtc ttggggaggc tcggtgcagg ctggggggtc
      841 tctgagactc tcctgcacag cccctggatt cacctccaat agctgccgca tggactggta
      901 ccgccaggct gcagggaagc agcgcgagtg ggtctcatct attagtactg atggtcgcac
      961 aagctatgca gactccgtga agggccgatt caccatctcc aaagacaaag ccaaggacac
     1021 ggtgtatctg caaatgaaca gcctgaaacc tgaggacacg gccatctatt actgtgccgt
     1081 gaggacgaat gggtatcgtc cgcaatctca cgaatttcgc tactggggcc cggggaccca
     1141 ggtcaccgtc tcctcagcgg ccgcatcggg ggccgcgtcg acgtacccat acgatgttcc
     1201 agattacgct ctggaagttc tgttccaggg gcccgcttcg acgcccgggg gtgcgccggt
     1261 gccgtatccg gatccgctgg aaccggccgg ggaaaattct cttgctaaaa atgtattatc
     1321 cggtggaaaa ggtaatgaca agttgtacgg cagtgaggga gcagacctgc ttgatggcgg
     1381 agaagggaat gatcttctga aaggtggata tggtaatgat atttatcgtt atctttcagg
     1441 atatggccat catattattg acgatgaagg ggggaaagac gataaactca gtttagctga
     1501 tatagatttc cgggacgttg cctttaagcg agaagggaat gacctcatta tgtataaagc
     1561 tgaaggtaat gttctttcta ttggccacaa aaatggtatt acatttaaaa actggtttga
     1621 aaaagagtca gatgatctct ctaatcatca gatagagcag atttttgata aagacggcag
     1681 ggtaatcaca ccagattctc ttaaaaaagc atttgaatat cagcagagta ataacaaggt
     1741 aagttatgtg tatggacatg atgcatcaac ttatgggagc caggacaatc ttaatccatt
     1801 aattaatgaa atcagcaaaa tcatttcagc tgcaggtaac ttcgatgtta aggaggaaag
     1861 atctgccgct tctttattgc agttgtccgg taatgccagt gatttttcat atggacggaa
     1921 ctcaataact ttgacagcat cagcataata tattaattta aatgatagca atcttactgg
     1981 gctgtgccaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2041 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2101 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     2161 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     2221 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2281 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2341 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     2401 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2461 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2521 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2581 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2641 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2701 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2761 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2821 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2881 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2941 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3001 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3061 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3121 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3181 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3241 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3301 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3361 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3421 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3961 cagcccagct tggagcgaac gacctacacc
//
