LOCUS       40924_17289        4620 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEGSH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Zhang C-F., Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Zhang C-F., Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 4620)
  AUTHORS   Zhang C-F., Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2018) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   4  (bases 1 to 4620)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4620)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAR-1999) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (06-JUN-2018) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jun 6, 2018 this sequence version replaced AF104248.2.
FEATURES             Location/Qualifiers
     source          1..4620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        293..438
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             481..1515
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    1559..1607
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      complement(1933..2521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2695..3552)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3560..3664)
                     /label=AmpR promoter
     rep_origin      3691..4146
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ataataaacc caagcttggc actgggatct gcgaacgcca gcaagacgta gcccagcgcg
       61 tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
      121 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
      181 gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg
      241 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
      301 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
      361 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
      421 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
      481 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
      541 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
      601 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
      661 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
      721 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
      781 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
      841 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
      901 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
      961 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     1021 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     1081 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     1141 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     1201 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     1261 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     1321 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     1381 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     1441 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
     1501 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccg cgctatgacg
     1561 gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt
     1621 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg ccaatacgcc
     1681 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     1741 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
     1801 ccactagcta gttctagtat gcatggcggt aatacggtta tccacagaat caggggataa
     1861 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1921 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1981 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2041 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2101 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2161 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2221 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2281 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2341 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2401 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2461 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2521 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2581 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2641 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctgaca gttaccaatg
     2701 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2761 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2821 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2881 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2941 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3001 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3061 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3121 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3181 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3241 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3301 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3361 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3421 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttcagg
     3481 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3541 ttgaatactc atcctcagga ctctcccttt ttcaatatta ttgaagcatt tatcagggtt
     3601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3661 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     3721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3841 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3901 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4021 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4081 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4141 caatttacgc gtatagatct cgcatattaa gtgcattgtt ctcgataccg ctaagtgcat
     4201 tgttctcgtt agctcgatgg acaagtgcat tgttctcttg ctgaaagctc gatggacaag
     4261 tgcattgttc tcttgctgaa agctcgatgg acaagtgcat tgttctcttg ctgaaagctc
     4321 agtacccggg tcggagtact gccccgcccc tagcgattag ccccggcccc gcatagctcc
     4381 gccccgggag taccctcgac cgccggagta taaatagagg cgcttcgtct acggagcgac
     4441 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     4501 gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa
     4561 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt
//
