LOCUS       40924_17284        5121 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEGPF-N2-NRSE4x, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 5121)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    complement(665..786)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    900..1012
                     /note="NRSE sequence; 4 fold"
     CDS             1067..1783
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    1909..2030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2037..2492)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2519..2623
                     /label=AmpR promoter
     promoter        2625..2982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3017..3808
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4043..4090
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4419..5007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagctctag agtcggggcg gccggccgct tcgagcagac
      661 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      721 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      781 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      841 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcctcgagct
      901 tcagcaccgc ggacagtgcc ttgtcgagct tcagcaccgc ggacagtgcc ttgtcgagct
      961 tcagcaccgc ggacagtgcc ttgtcgagct tcagcaccgc ggacagtgcc ttgtcgagat
     1021 ccgtcgacgg taccgcgggc ccgggatcca ccggccggtc gccaccatgg tgagcaaggg
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1261 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1501 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     1801 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1861 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1921 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1981 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2041 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2101 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2161 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2221 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2281 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2341 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2401 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2461 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     2641 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2701 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2821 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2881 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2941 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3001 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3061 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3121 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3181 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3241 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3301 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3361 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3421 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3481 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3541 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3601 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3661 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3721 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3781 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3841 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3961 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4021 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4081 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4141 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4201 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4261 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4561 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5101 aaccgtatta ccgccatgca t
//
