LOCUS       62056_9110       10308 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..678
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             746..1768
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1811..1985
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2063..2087)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2512..3303
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3393..3981
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4167..4307)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4651..4845)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4910..5974)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6411..7037)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(8337..8361)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        9008..9352
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9708..9960
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(10052..10082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10097..10118)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gataacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
      301 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
      361 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
      421 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
      481 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
      541 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
      601 tgacgcacaa tcccactatc cttcgcaaga ccttcctcta tataaggaag ttcatttcat
      661 ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt
      721 cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
      781 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
      841 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
      901 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
      961 cccgattccg gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc
     1021 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     1081 acaaccggtc gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     1141 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     1201 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     1261 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     1321 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     1381 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     1441 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     1501 gcatccggag cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga
     1561 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     1621 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     1681 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     1741 ccccagcact cgtccgaggg caaagaaata gagtagatgc cgaccggatc tgtcgatcga
     1801 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     1861 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     1921 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     1981 agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     2041 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     2101 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     2161 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     2221 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     2281 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat
     2341 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
     2401 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
     2461 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
     2521 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
     2581 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
     2641 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
     2701 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
     2761 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
     2821 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
     2881 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
     2941 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
     3001 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
     3061 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
     3121 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
     3181 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
     3241 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
     3301 ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3361 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3421 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3481 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3541 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3601 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3661 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3721 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3781 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3841 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3901 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3961 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4021 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4081 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4141 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4201 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4261 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     4321 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4381 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4441 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     4501 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     4561 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     4621 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     4681 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     4741 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     4801 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa ccttttcgac
     4861 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     4921 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     4981 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     5041 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     5101 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     5161 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     5221 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     5281 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     5341 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     5401 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     5461 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     5521 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     5581 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     5641 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     5701 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     5761 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     5821 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     5881 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     5941 cgccgatttg taccgggccg gatggtttgc gaccgtcacg ccgattcctc gggcttgggg
     6001 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     6061 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     6121 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     6181 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     6241 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     6301 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     6361 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     6421 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     6481 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     6541 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     6601 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     6661 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     6721 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     6781 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     6841 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     6901 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     6961 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     7021 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     7081 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     7141 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     7201 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     7261 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     7321 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     7381 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     7441 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     7501 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     7561 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     7621 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     7681 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
     7741 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
     7801 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
     7861 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
     7921 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
     7981 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
     8041 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
     8101 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
     8161 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
     8221 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
     8281 accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttaggttt
     8341 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
     8401 tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg
     8461 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8521 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     8581 gccaagctta ccgatgcctg caggtcaaca tggtggagca cgacacactt gtctactcca
     8641 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
     8701 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
     8761 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
     8821 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     8881 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg
     8941 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
     9001 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
     9061 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
     9121 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
     9181 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     9241 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     9301 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acctcgacct
     9361 caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca
     9421 gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg
     9481 aacgatagcc accatgggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     9541 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     9601 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     9661 gatcactctc ggcatggacg agctgtacaa gtgacggtga tcctcccgat cgttcaaaca
     9721 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     9781 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     9841 tgaggtgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     9901 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     9961 gggagcaccg gtaaggcgcg ccgtagtgag aattcgtaat catgtcatag ctgtttcctg
    10021 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10081 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10141 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10201 gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt
    10261 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctat
//
