LOCUS       V014751                 5650 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014751
VERSION     V014751
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             657..1574
                     /gene="PABPN1"
                     /label="Polyadenylate-binding protein 2"
                     /note="Polyadenylate-binding protein 2 from Homo sapiens.
                     Accession#: Q86U42"
     CDS             1596..2312
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2438..2559
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2566..3021)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3048..3152
                     /label="AmpR promoter"
     promoter        3154..3511
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3546..4337
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4572..4619
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgg
      661 cggcggcggc ggcggcggca gcagcagcgg gggctgcggg cggtcggggc tccgggccgg
      721 ggcggcggcg ccatcttgtg cccggggccg gtggggaggc cggggagggg gccccggggg
      781 gcgcagggga ctacgggaac ggcctggagt ctgaggaact ggagcctgag gagctgctgc
      841 tggagcccga gccggagccc gagcccgaag aggagccgcc ccggccccgc gcccccccgg
      901 gagctccggg ccctgggcct ggttcgggag cccccggcag ccaagaggag gaggaggagc
      961 cgggactggt cgagggtgac ccgggggacg gcgccattga ggacccggag ctggaagcta
     1021 tcaaagctcg agtcagggag atggaggaag aagctgagaa gctaaaggag ctacagaacg
     1081 aggtagagaa gcagatgaat atgagtccac ctccaggcaa tgctggcccg gtgatcatgt
     1141 ccattgagga gaagatggag gctgatgccc gttccatcta tgttggcaat gtggactatg
     1201 gtgcaacagc agaagagctg gaagctcact ttcatggctg tggttcagtc aaccgtgtta
     1261 ccatactgtg tgacaaattt agtggccatc ccaaagggtt tgcgtatata gagttctcag
     1321 acaaagagtc agtgaggact tccttggcct tagatgagtc cctatttaga ggaaggcaaa
     1381 tcaaggtgat cccaaaacga accaacagac caggcatcag cacaacagac cggggttttc
     1441 cacgagcccg ctaccgcgcc cggaccacca actacaacag ctcccgctct cgattctaca
     1501 gtggttttaa cagcaggccc cggggtcgcg tctacagggg ccgggctaga gcgacatcat
     1561 ggtattcccc ttacacggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     1621 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1681 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1741 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1801 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1861 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1921 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1981 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2041 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2101 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2161 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2221 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2281 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat
     2341 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2401 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     2581 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2641 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2701 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2761 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     2821 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     2881 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2941 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3001 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3121 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     3181 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3241 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3361 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3481 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     3541 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3601 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3661 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3721 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3781 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3841 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3901 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3961 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4021 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4081 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4141 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4201 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4261 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4321 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4381 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4441 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4501 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4561 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     4621 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4681 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4741 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     4801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5641 cgccatgcat
//
