LOCUS       62056_9060        6638 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3426..3547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3554..4009)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4036..4140
                     /label=AmpR promoter
     promoter        4142..4499
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4534..5325
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5560..5607
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5936..6524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagac atgccttgtg ttcaggcgca gtatgggtcc
     1381 tcgcctcaag gagccagccc cgcttctcag agctacagtt accactcttc gggagaatac
     1441 agctccgatt tcttaactcc agagtttgtc aagtttagca tggacctcac caacactgaa
     1501 atcactgcca ccacttctct ccccagcttc agtaccttta tggacaacta cagcacaggc
     1561 tacgacgtca agccaccttg cttgtaccaa atgcccctgt ccggacagca gtcctccatt
     1621 aaggtagaag acattcagat gcacaactac cagcaacaca gccacctgcc cccccagtct
     1681 gaggagatga tgccgcactc cgggtcggtt tactacaagc cctcctcgcc cccgacgccc
     1741 accaccccgg gcttccaggt gcagcacagc cccatgtggg acgacccggg atctctccac
     1801 aacttccacc agaactacgt ggccactacg cacatgatcg agcagaggaa aacgccagtc
     1861 tcccgcctct ccctcttctc ctttaagcaa tcgccccctg gcaccccggt gtctagttgc
     1921 cagatgcgct tcgacgggcc cctgcacgtc cccatgaacc cggagcccgc cggcagccac
     1981 cacgtggtgg acgggcagac cttcgctgtg cccaacccca ttcgcaagcc cgcgtccatg
     2041 ggcttcccgg gcctgcagat cggccacgcg tctcagctgc tcgacacgca ggtgccctca
     2101 ccgccgtcgc ggggctcccc ctccaacgag gggctgtgcg ctgtgtgtgg ggacaacgcg
     2161 gcctgccaac actacggcgt gcgcacctgt gagggctgca aaggcttctt taagcgcaca
     2221 gtgcaaaaaa atgcaaaata cgtgtgttta gcaaataaaa actgcccagt ggacaagcgt
     2281 cgccggaatc gctgtcagta ctgccgattt cagaagtgcc tggctgttgg gatggtcaaa
     2341 gaagtggttc gcacagacag tttaaaaggc cggagaggtc gtttgccctc gaaaccgaag
     2401 agcccacagg agccctctcc cccttcgccc ccggtgagtc tgatcagtgc cctcgtcagg
     2461 gcccatgtcg actccaaccc ggctatgacc agcctggact attccaggtt ccaggcgaac
     2521 cctgactatc aaatgagtgg agatgacacc cagcatatcc agcaattcta tgatctcctg
     2581 actggctcca tggagatcat ccggggctgg gcagagaaga tccctggctt cgcagacctg
     2641 cccaaagccg accaagacct gctttttgaa tcagctttct tagaactgtt tgtccttcga
     2701 ttagcataca ggtccaaccc agtggagggt aaactcatct tttgcaatgg ggtggtcttg
     2761 cacaggttgc aatgcgttcg tggctttggg gaatggattg attccattgt tgaattctcc
     2821 tccaacttgc agaatatgaa catcgacatt tctgccttct cctgcattgc tgccctggct
     2881 atggtcacag gtcagtactg caggcgcagg gcgcttcccc tccagaactg cctagcagga
     2941 tttgtcctga gtttcccttg tcacagattc tccttggttt tgccaactag ctaactgtct
     3001 tgtacattct tcttttgttt ctgattatgt tttctgcaga gagacacggg ctcaaggaac
     3061 ccaagagagt ggaagaactg caaaacaaga ttgtaaattg tctcaaagac cacgtgactt
     3121 tcaacaatgg ggggttgaac cgccccaatt atttgtccaa actgttgggg aagctcccag
     3181 aacttcgtac cctttgcaca caggggctac agcgcatttt ctacctgaaa ttggaagact
     3241 tggtgccacc gccagcaata attgacaaac ttttcctgga cactttacct ttctaaggat
     3301 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3361 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3421 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3541 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3601 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3661 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3721 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3781 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3841 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3901 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3961 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4141 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4201 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4321 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4381 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4441 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4501 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4561 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4621 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4681 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4741 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4801 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4861 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4921 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4981 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5041 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     5101 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5161 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5221 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5281 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5341 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5401 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5461 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5521 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5581 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5641 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5701 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5761 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5821 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5881 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5941 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6001 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6061 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6121 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6181 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6241 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6301 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6361 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6421 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6481 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6541 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6601 cccctgattc tgtggataac cgtattaccg ccatgcat
//
