LOCUS       40924_17194        4237 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEGFP-N2-NRSE4x-ohne-CMV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 4237)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..128
                     /note="NRSE sequence; 4 fold"
     CDS             183..899
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    1025..1146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1153..1608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1635..1739
                     /label=AmpR promoter
     promoter        1741..2098
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2133..2924
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3159..3206
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3535..4123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattat cgagcttcag caccgcggac agtgccttgt cgagcttcag caccgcggac
       61 agtgccttgt cgagcttcag caccgcggac agtgccttgt cgagcttcag caccgcggac
      121 agtgccttgt cgagatccgt cgacggtacc gcgggcccgg gatccaccgg ccggtcgcca
      181 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      241 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      301 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      361 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      421 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      481 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      541 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      601 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      661 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      721 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      781 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      841 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
      901 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
      961 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     1021 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1081 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1141 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     1201 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     1261 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     1321 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     1381 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     1441 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1501 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     1561 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     1621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     1741 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1801 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1861 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1921 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     2101 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2281 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     2341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2641 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     2761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     2821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     2881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     2941 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3001 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3061 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     3121 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     3181 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     3241 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     3301 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     3361 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     3421 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3661 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4201 ccctgattct gtggataacc gtattaccgc catgcat
//
