LOCUS       V007597                 6807 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007597
VERSION     V007597
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6807)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             629..2731
                     /gene="TRAP1"
                     /label="Heat shock protein 75 kDa, mitochondrial"
                     /note="Heat shock protein 75 kDa, mitochondrial from Homo
                     sapiens. Accession#: Q12931"
     CDS             2753..3469
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3595..3716
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3723..4178)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4205..4309
                     /label="AmpR promoter"
     promoter        4311..4668
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4703..5494
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5729..5776
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6105..6693
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attccgggcg atgctgctgt ggggccgccg
      661 cctgcggcct ttgctgcggg cgccggcgct ggcggccgtg ccgggaggaa aaccaattct
      721 gtgtcctcgg aggaccacag cccagttggg ccccaggcga aacccagcct ggagcttgca
      781 ggcaggacga ctgttcagca cgcagaccgc cgaggacaag gaggaacccc tgcactcgat
      841 tatcagcagc acagagagcg tgcagggttc cacttccaaa catgagttcc aggccgagac
      901 aaagaagctt ttggacattg ttgcccggtc cctgtactca gaaaaagagg tgtttatacg
      961 ggagctgatc tccaatgcca gcgatgcctt ggaaaaactg cgtcacaaac tggtgtctga
     1021 cggccaagca ctgccagaaa tggagattca cttgcagacc aatgccgaga aaggcaccat
     1081 caccatccag gatactggta tcgggatgac acaggaagag ctggtgtcca acctggggac
     1141 gattgccaga tcggggtcaa aggccttcct ggatgctctg cagaaccagg ctgaggccag
     1201 cagcaagatc atcggccagt ttggagtggg tttctactca gctttcatgg tggctgacag
     1261 agtggaggtc tattcccgct cggcagcccc ggggagcctg ggttaccagt ggctttcaga
     1321 tggttctgga gtgtttgaaa tcgccgaagc ttcgggagtt agaaccggga caaaaatcat
     1381 catccacctg aaatccgact gcaaggagtt ttccagcgag gcccgggtgc gagatgtggt
     1441 aacgaagtac agcaacttcg tcagcttccc cttgtacttg aatggaaggc ggatgaacac
     1501 cttgcaggcc atctggatga tggaccccaa ggatgtcggt gagtggcaac atgaggagtt
     1561 ctaccgctac gtcgcgcagg ctcacgacaa gccccgctac accctgcact ataagacgga
     1621 cgcaccgctc aacatccgca gcatcttcta cgtgcccgac atgaaaccgt ccatgtttga
     1681 tgtgagccgg gagctgggct ccagcgttgc actgtacagc cgcaaagtcc tcatccagac
     1741 caaggccacg gacatcctgc ccaagtggct gcgcttcatc cgaggtgtgg tggacagtga
     1801 ggacattccc ctgaacctca gccgggagct gctgcaggag agcgcactca tcaggaaact
     1861 ccgggacgtt ttacagcaga ggctgatcaa attcttcatt gaccagagta aaaaagatgc
     1921 tgagaagtat gcaaagtttt ttgaagatta cggcctgttc atgcgggagg gcattgtgac
     1981 cgccaccgag caggaggtca aggaggacat agcaaagctg ctgcgctacg agtcctcggc
     2041 gctgccctcc gggcagctaa ccagcctctc agaatacgcc agccgcatgc gggccggcac
     2101 ccgcaacatc tactacctgt gcgcccccaa ccgtcacctg gcagagcact caccctacta
     2161 tgaggccatg aagaagaaag acacagaggt tctcttctgc tttgagcagt ttgatgagct
     2221 caccctgctg caccttcgtg agtttgacaa gaagaagctg atctctgtgg agacggacat
     2281 agtcgtggat cactacaagg aggagaagtt tgaggacagg tccccagccg ccgagtgcct
     2341 atcagagaag gagacggagg agctcatggc ctggatgaga aatgtgctgg ggtcgcgtgt
     2401 caccaacgtg aaggtgaccc tccgactgga cacccaccct gccatggtca ccgtgctgga
     2461 gatgggggct gcccgccact tcctgcgcat gcagcagctg gccaagaccc aggaggagcg
     2521 cgcacagctc ctgcagccca cgctggagat caaccccagg cacgcgctca tcaagaagct
     2581 gaatcagctg cgcgcaagcg agcctggcct ggctcagctg ctggtggatc agatatacga
     2641 gaacgccatg attgctgctg gacttgttga cgaccctagg gccatggtgg gccgcttgaa
     2701 tgagctgctt gtcaaggccc tggagcgaca ccgggatcca ccggtcgcca ccatggtgag
     2761 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2821 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2881 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     2941 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3001 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3061 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3121 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3181 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3241 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3301 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3361 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3421 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     3481 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3541 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     3721 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3781 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3841 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3901 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3961 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4021 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4081 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4141 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     4201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     4321 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4441 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4501 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4621 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4681 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4861 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5221 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5521 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5641 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5701 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5761 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5821 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5881 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5941 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6241 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6781 gtggataacc gtattaccgc catgcat
//
