LOCUS       40924_17184        6654 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pEGFP-N1-TRAP1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6654)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6654)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             641..2578
                     /codon_start=1
                     /note="unnamed protein product; mature hTRAP1"
                     /protein_id="SJL86614.1"
                     /translation="MSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVAR
                     SLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIG
                     MTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRS
                     AAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNF
                     VSFPLYLNGRRMNTLQAIWMMDPKDVGEWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNI
                     RSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPL
                     NLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATE
                     QEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEA
                     MKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLS
                     EKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEER
                     AQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRL
                     NELLVKALERH"
     CDS             2600..3316
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    3442..3563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3570..4025)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4052..4156
                     /label=AmpR promoter
     promoter        4158..4515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4550..5341
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5576..5623
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5952..6540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccgcc atgagcacgc agaccgccga
      661 ggacaaggag gaacccctgc actcgattat cagcagcaca gagagcgtgc agggttccac
      721 ttccaaacat gagttccagg ccgagacaaa gaagcttttg gacattgttg cccggtccct
      781 gtactcagaa aaagaggtgt ttatacggga gctgatctcc aatgccagcg atgccttgga
      841 aaaactgcgt cacaaactgg tgtctgacgg ccaagcactg ccagaaatgg agattcactt
      901 gcagaccaat gccgagaaag gcaccatcac catccaggat actggtatcg ggatgacaca
      961 ggaagagctg gtgtccaacc tggggacgat tgccagatcg gggtcaaagg ccttcctgga
     1021 tgctctgcag aaccaggctg aggccagcag caagatcatc ggccagtttg gagtgggttt
     1081 ctactcagct ttcatggtgg ctgacagagt ggaggtctat tcccgctcgg cagccccggg
     1141 gagcctgggt taccagtggc tttcagatgg ttctggagtg tttgaaatcg ccgaagcttc
     1201 gggagttaga accgggacaa aaatcatcat ccacctgaaa tccgactgca aggagttttc
     1261 cagcgaggcc cgggtgcgag atgtggtaac gaagtacagc aacttcgtca gcttcccctt
     1321 gtacttgaat ggaaggcgga tgaacacctt gcaggccatc tggatgatgg accccaagga
     1381 tgtcggtgag tggcaacatg aggagttcta ccgctacgtc gcgcaggctc acgacaagcc
     1441 ccgctacacc ctgcactata agacggacgc accgctcaac atccgcagca tcttctacgt
     1501 gcccgacatg aaaccgtcca tgtttgatgt gagccgggag ctgggctcca gcgttgcact
     1561 gtacagccgc aaagtcctca tccagaccaa ggccacggac atcctgccca agtggctgcg
     1621 cttcatccga ggtgtggtgg acagtgagga cattcccctg aacctcagcc gggagctgct
     1681 gcaggagagc gcactcatca ggaaactccg ggacgtttta cagcagaggc tgatcaaatt
     1741 cttcattgac cagagtaaaa aagatgctga gaagtatgca aagttttttg aagattacgg
     1801 cctgttcatg cgggagggca ttgtgaccgc caccgagcag gaggtcaagg aggacatagc
     1861 aaagctgctg cgctacgagt cctcggcgct gccctccggg cagctaacca gcctctcaga
     1921 atacgccagc cgcatgcggg ccggcacccg caacatctac tacctgtgcg cccccaaccg
     1981 tcacctggca gagcactcac cctactatga ggccatgaag aagaaagaca cagaggttct
     2041 cttctgcttt gagcagtttg atgagctcac cctgctgcac cttcgtgagt ttgacaagaa
     2101 gaagctgatc tctgtggaga cggacatagt cgtggatcac tacaaggagg agaagtttga
     2161 ggacaggtcc ccagccgccg agtgcctatc agagaaggag acggaggagc tcatggcctg
     2221 gatgagaaat gtgctggggt cgcgtgtcac caacgtgaag gtgaccctcc gactggacac
     2281 ccaccctgcc atggtcaccg tgctggagat gggggctgcc cgccacttcc tgcgcatgca
     2341 gcagctggcc aagacccagg aggagcgcgc acagctcctg cagcccacgc tggagatcaa
     2401 ccccaggcac gcgctcatca agaagctgaa tcagctgcgc gcaagcgagc ctggcctggc
     2461 tcagctgctg gtggatcaga tatacgagaa cgccatgatt gctgctggac ttgttgacga
     2521 ccctagggcc atggtgggcc gcttgaatga gctgcttgtc aaggccctgg agcgacaccg
     2581 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2701 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3001 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3061 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3121 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3181 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3241 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3301 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     3361 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3421 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3541 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3601 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3661 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3721 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3781 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3841 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3901 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3961 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4021 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4141 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4441 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4501 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4561 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4621 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4681 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4741 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4801 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4861 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4921 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4981 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5041 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     5101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5341 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5401 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5461 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5521 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     5581 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     5641 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     5701 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     5761 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     5821 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6061 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
