LOCUS       V013272                 6833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013272
VERSION     V013272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             625..2754
                     /gene="FASTKD2"
                     /label="FAST kinase domain-containing protein 2,
                     mitochondrial"
                     /note="FAST kinase domain-containing protein 2,
                     mitochondrial from Homo sapiens. Accession#: Q9NYY8"
     CDS             2779..3495
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3621..3742
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3749..4204)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4231..4335
                     /label="AmpR promoter"
     promoter        4337..4694
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4729..5520
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5755..5802
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6131..6719
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgttg acaactttga agccatttgg aagtgtttca
      661 gtggagagca aaatgaataa caaagcgggc tcctttttct ggaaccttag acaattcagt
      721 acattagttt caacaagcag aactatgagg ctatgttgtt tgggactttg caaaccaaaa
      781 atagttcatt caaactggaa cattttaaat aactttcata acagaatgca atcaactgat
      841 atcattagat atctctttca ggatgcattc atttttaaat cagatgttgg ctttcaaaca
      901 aagggcataa gcactctaac agcccttaga attgaaagac tactttatgc taaaagactg
      961 ttttttgact caaagcagtc tcttgtccct gttgataaat ctgatgatga attgaagaaa
     1021 gtaaacctta atcatgaagt ctccaatgaa gatgttctta ccaaggaaac aaaaccaaac
     1081 cgtatcagca gtagaaaact gtctgaggaa tgtaattccc tgagtgatgt gttagatgca
     1141 ttttcaaaag cgcccacatt tcctagtagc aactatttca cagcaatgtg gacaattgcc
     1201 aaaagactgt ctgatgacca gaagcgcttt gaaaaacgac tgatgtttag ccaccctgca
     1261 tttaatcagc tctgtgaaca tatgatgaga gaagccaaga tcatgcagta taagtaccta
     1321 ctgttcagtc ttcacgccat agtgaagctt ggaatccctc agaacactat tttggtgcag
     1381 actttgctga gggtgaccca ggaacgtatc aatgagtgtg atgagatatg cctttcagtt
     1441 ttgtcaactg ttttagaggc aatggaacca tgcaagaatg ttcatgttct acgaacggga
     1501 ttcagaatac tagttgatca gcaagtttgg aaaatagaag atgtcttcac attacaagtt
     1561 gtgatgaagt gtattggaaa agatgcaccg attgctctta agaggaaact ggagatgaaa
     1621 gccttgaggg aattagacag attttctgtt ttgaatagcc aacacatgtt tgaagtacta
     1681 gctgccatga atcaccgatc tcttatactc ctggatgaat gcagtaaggt ggtcctagat
     1741 aatatccatg ggtgtccttt aagaataatg atcaacatat tgcagtcctg caaagacctc
     1801 cagtaccata atttggatct cttcaaggga cttgcagatt atgtggctgc aactttcgac
     1861 atctggaagt tcagaaaagt tctttttatc ctcattttat ttgaaaacct tggctttcga
     1921 cctgttggtt taatggacct gtttatgaag agaatagtag aggatcctga atccctaaac
     1981 atgaaaaaca ttctatctat tcttcatact tactcttctc tcaatcatgt ctacaaatgc
     2041 cagaacaaag aacagttcgt ggaagttatg gctagtgctc tgactggtta tcttcacact
     2101 atttcttctg aaaacttatt ggatgcagta tattcatttt gcttgatgaa ttactttccc
     2161 ctggctcctt ttaatcagct tctgcaaaaa gacatcatca gtgagctgct gacatcagat
     2221 gacatgaaga atgcttacaa gctgcatact ttggatactt gtctaaaact tgatgatact
     2281 gtctatctga gggacatagc cttgtcactc ccacagctgc cgcgggagct gccatcgtca
     2341 catacaaatg caaaggtggc agaggtgctg agcagccttc tgggaggtga aggacacttc
     2401 tcaaaggatg tgcacttgcc acacaattat catattgatt ttgaaatcag aatggacact
     2461 aacaggaatc aagtgctacc actttctgat gtggatacaa cttctgctac agatattcaa
     2521 agagtagctg tgctatgtgt ttccagatct gcttattgtt tgggttcaag ccaccccaga
     2581 ggattccttg ctatgaaaat gcggcatttg aatgcaatgg gttttcatgt gatcttggtc
     2641 aataactggg agatggacaa actagagatg gaagatgcag tcacattttt gaagactaaa
     2701 atctattcag tagaagctct tcctgttgct gctgtaaatg tgcaaagcac acaatcccgg
     2761 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2821 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2881 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2941 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3001 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3061 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3121 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3181 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3241 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3301 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3361 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3421 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3481 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
     3541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3721 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     3781 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3841 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3961 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4021 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4081 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4141 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4201 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4261 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4321 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4681 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4741 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4801 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4861 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4921 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4981 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5041 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5101 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5161 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5221 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     5281 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5341 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5401 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5461 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5521 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5581 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5641 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     5701 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     5761 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5821 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5881 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5941 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     6001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6241 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
