LOCUS       Exported               12464 bp DNA     circular SYN 12-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2728..3073
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3088..3804
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3960..4212
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4500..4845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4862..5413
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      5436..5688
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5697..5713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6331..6355
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(7951..9099)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9398..10192)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  10384..11151
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(11772..12464,1..18)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc gggcaattga gacttttcaa caaagggtaa tatccggaaa
     2761 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     2821 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     2881 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     2941 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     3001 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaagaa gttcatttca
     3061 tttggagaga acacggggga cgagctcatg gtgagcaagg gcgaggagct gttcaccggg
     3121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3301 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3541 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3601 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3661 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3781 ctcggcatgg acgagctgta caagaagaag ggcggaaaga tcgccgtggg aggtggctca
     3841 tctggcggag gtcagatctc gtacgcgtcc cggggcggta cccgggatcc tctagagggc
     3901 ccggcgcgcc actagtgtcg acctgcagct gatctcgagg agctagctcg aatttccccg
     3961 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     4021 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     4081 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     4141 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     4201 tgttactaga tcgggagtac tattccagta tggacgattc aaggcttgct tcacaaacca
     4261 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg
     4321 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
     4381 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
     4441 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
     4501 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     4561 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     4621 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     4681 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     4741 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4801 gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg gactctagac
     4861 catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg acatgccggc
     4921 ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc gtaccgagcc
     4981 gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct atccctggct
     5041 cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct ggaaggcacg
     5101 caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac
     5161 gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa
     5221 gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct
     5281 cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga
     5341 cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg tcctgcccgt
     5401 caccgagatc tgaggtaccg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     5461 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     5521 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     5581 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     5641 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattcactg
     5701 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5761 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5821 tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc gctttcttcc cttcctttct
     5881 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5941 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     6001 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6061 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     6121 tttataaggg attttgccga tttcggaacc accatcaaac aggattttcg cctgctgggg
     6181 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     6241 ctgttgcccg tctcactggt gaaaagaaaa accaccccag tacattaaaa acgtccgcaa
     6301 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     6361 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     6421 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6481 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6541 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaaatatca
     6601 tctccctcgc agagatccga attatcagcc ttcttattca tttctcgctt aaccgtgaca
     6661 ggctgtcgat cttgagaact atgccgacat aataggaaat cgctggataa agccgctgag
     6721 gaagctgagt ggcgctattt ctttagaagt gaacgttgac gatatcaact cccctatcca
     6781 ttgctcaccg aatggtacag gtcggggacc cgaagttccg actgtcggcc tgatgcatcc
     6841 ccggctgatc gaccccagat ctggggctga gaaagcccag taaggaaaca actgtaggtt
     6901 cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg atcaggccga
     6961 gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga tcaaacacta
     7021 aagctactgg aacgagcaga agtcctccgg ccgccagttg ccaggcggta aaggtgagca
     7081 gaggcacggg aggttgccac ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc
     7141 ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca
     7201 gcgccacgcc cgcagttccg caaatagccc ccaggaccgc catcaatcgt atcgggctac
     7261 ctagcagagc ggcagagatg aacacgacca tcagcggctg cacagcgcct accgtcgccg
     7321 cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa gctccagaat agcgaaatat
     7381 taagtgcgcc gaggatgaag atgcgcatcc accagattcc cgttggaatc tgtcggacga
     7441 tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg acccctcggc
     7501 ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg tccttggggc
     7561 cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg
     7621 ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt
     7681 aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc tcgcggaaat
     7741 cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt gtcaatttta
     7801 atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa
     7861 gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga
     7921 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
     7981 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
     8041 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
     8101 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
     8161 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
     8221 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
     8281 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
     8341 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
     8401 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
     8461 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
     8521 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
     8581 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
     8641 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
     8701 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
     8761 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
     8821 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
     8881 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
     8941 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
     9001 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
     9061 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
     9121 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
     9181 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
     9241 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata
     9301 cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc
     9361 ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca tccagtaaaa
     9421 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     9481 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     9541 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     9601 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     9661 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     9721 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     9781 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     9841 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     9901 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     9961 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    10021 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    10081 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    10141 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
    10201 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    10261 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    10321 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt
    10381 atgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
    10441 ggcttctgtg tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
    10501 caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact
    10561 gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat
    10621 gaatggcgtt ggatgccggg caacagcccg cattatgggc gttggcctca acacgatttt
    10681 acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac
    10741 gtcatcgtct gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag
    10801 cgctggctgt tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt
    10861 gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg
    10921 atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta
    10981 atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg
    11041 gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
    11101 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac ccaattatga
    11161 tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt
    11221 ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg
    11281 aaatccttac atacggcgat atcttctata taaaagatat attatcttat cagtattgtc
    11341 aatatattca aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt
    11401 taaatatggc gcttcataga gtaattctgt aaaggtccaa ttctcgtttc atacctcggt
    11461 ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg aacacgagca
    11521 cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc cccgccatga
    11581 agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccaccaggc cgccgccctc
    11641 actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc
    11701 gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg caatggcaag
    11761 gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
    11821 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
    11881 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
    11941 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
    12001 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
    12061 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctatctgt cagtagtcgc
    12121 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
    12181 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
    12241 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
    12301 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
    12361 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
    12421 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccgg
//
