LOCUS       V007603                 4151 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007603
VERSION     V007603
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Zhou Y, Zhang L, Shah N, Palmer R Jr.
  TITLE     Cell-surface glycoside hydrolases of Streptococcus gordonii promote
            growth in saliva
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Yang J, Cisar JO.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2015) Laboratory of Cell and Developmental
            Biology, National Institute of Health, 9000 Rockville Pike,
            Bethesda, MD 20854, USA
REFERENCE   3  (bases 1 to 4151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2015) Laboratory of Cell and Developmental Biology, National
            Institute of Health, 9000 Rockville Pike, Bethesda, MD 20854, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      402..599
                     /note="consititutive promoter located upstream of
                     Streptococcus gordonii rpsJ gene"
                     /regulatory_class="promoter"
     CDS             600..1313
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    1320..1376
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1389..1405)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1413..1429)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1437..1467)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1482..1503)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1791..2379)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2951..3685
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        complement(3952..4056)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctaaaaatag ttaaaaaaat
      421 ctctcaaatc ccttgcaatg cacgcatctt tgtgttaaga tactatggtg ctgtaaaaat
      481 acagcgtgta gctttgatgc aagaggttgc gacacgctcg gttgcattgc cacgcaaccg
      541 cctgtcggtt ttcttgtgga gctagcctat tatcttaaat agacgaaaag gagaaaaaga
      601 tgcgtaaagg cgaagaatta tttacagggg ttgtaccaat tttagttgaa ttagatggag
      661 atgttaacgg acataaattt tcagtaagcg gagagggtga aggcgatgca acttatggta
      721 aacttacact gaaattcatt tgcactaccg gaaaactacc tgtaccttgg ccaacgttag
      781 ttactacatt cggatacgga gtacaatgtt tcgcacgata tccagaccac atgaaacaac
      841 acgatttttt taaaagcgcg atgccagaag gttatgtaca agaacgaaca atatttttta
      901 aagatgacgg aaattacaaa acaagagcag aagtaaaatt tgaaggagat acacttgtaa
      961 atagaattga actaaaagga atagatttta aagaagacgg caatatccta ggacacaaac
     1021 ttgaatacaa ttataactct cacaatgtat atattatggc tgacaaacaa aaaaatggaa
     1081 ttaaagtaaa ttttaaaatt agacacaaca ttgaagatgg atcagtacaa ttagcggatc
     1141 actatcaaca aaatacacca attggcgatg gaccagtatt actcccagac aatcactacc
     1201 tttcaactca aagtgcacta tccaaagacc caaacgagaa acgcgatcac atggtactac
     1261 ttgaatttgt aactgctgca ggaataaccc acggaatgga cgaactctat aaataataag
     1321 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg
     1381 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1441 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1501 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1561 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2821 agtagttcgc cagttaatag tttgcgcaac gttaagagtg tgttgatagt gcagtatctt
     2881 aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt aattaagaag
     2941 gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
     3001 actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
     3061 aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
     3121 tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt
     3181 cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
     3241 tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
     3301 ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
     3361 tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
     3421 gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
     3481 ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
     3541 ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
     3601 cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
     3661 ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt
     3721 tacacgttac taaagggaat gtagataaat tattaggtat gaacgttctt cggggcgaaa
     3781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4081 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4141 gccctttcgt c
//
