LOCUS       V007618                 8069 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007618
VERSION     V007618
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8069)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8069)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        351..1529
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1546..1564
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1639..1677
                     /label="E-tag"
                     /note="E-tag"
     CDS             1687..3930
                     /gene="Pla2g4a"
                     /label="Cytosolic phospholipase A2"
                     /note="Cytosolic phospholipase A2 from Mus musculus.
                     Accession#: P47713"
     CDS             3951..3980
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3996..4013
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    4042..4266
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4312..4740
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4754..5083
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5131..5178
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5197..5592
                     /label="BSD"
                     /note="blasticidin S deaminase"
     polyA_signal    5753..5886
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5923..5939)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5947..5963)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5971..6001)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6016..6037)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6314..6902)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7076..7933)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7934..8038)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
     1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg
     1681 gccgctatgt ctttcataga tccttatcag cacattatag tggaacacca gtactcccat
     1741 aagtttactg ttgtggttct acgtgccacc aaagtaacca aggggacctt tggcgatatg
     1801 ctggacactc cagatcctta tgtggaactt ttcatctcta caacccctga cagcaggaag
     1861 cgaacgagac acttcaataa tgatataaac cccgtgtgga atgagacctt tgagttcatt
     1921 ttggatccta atcaggaaaa tgttttggag atcacactga tggatgccaa ctacgtcatg
     1981 gatgaaaccc taggcacagc tacattccct gtatcttcaa tgaaagtggg agagaagaaa
     2041 gaagtccctt ttattttcaa ccaagtcact gaaatgattc tggaaatgtc tctggaagtt
     2101 tgttcatgcc cagacctacg gttcagcatg gcactgtgtg atcaggagaa aactttcaga
     2161 cagcagagga aagagaacat aaaagagaac atgaagaaac ttttgggtcc aaaaaagagt
     2221 gaggggcttt attccacacg tgatgtgccg gtggtggcca ttttgggttc aggtgggggt
     2281 ttccgggcca tggtgggatt ctctggtgtg atgaaggcac tgtatgagtc ggggattttg
     2341 gactgtgcta catacattgc tggtctttct ggatccacat ggtacatgtc aaccttgtac
     2401 tctcaccccg attttccaga gaaaggtccc gaggagatta atgaagagct aatgaaaaat
     2461 gtcagccaca accctctctt acttcttaca ccacagaaag ttaaaagata cgttgagtct
     2521 ttatggaaga agaaaagttc tggccagcct gtcaccttta ctgacatctt tgggatgcta
     2581 ataggagaaa cactaattca aaataggatg agcatgaccc tgagtagttt gaaggaaaag
     2641 gtcaatgccg cccggtgtcc tttgcctctc ttcacgtgtc tccacgtcaa acctgatgtg
     2701 tcagagctga tgtttgccga ttgggtggaa tttagtccat atgagattgg catggcaaaa
     2761 tatggtacct ttatggctcc tgacctattt ggaagcaagt tttttatggg aacagttgta
     2821 aaaaaatatg aagaaaaccc cttgcatttc ttgatgggtg tctggggcag tgccttttct
     2881 atactgttca acagagtttt gggagtttct ggctcacaga ataaaggctc tacaatggaa
     2941 gaggaattag aaaatattac agcaaagcac atcgtgagta atgacagctc cgacagtgat
     3001 gatgaggctc aaggacccaa aggcaccgag aatgaagaag ctgaaaaaga gtaccaaagc
     3061 gacaaccaag caagttgggt ccatcggatg ctaatggcct tggtgagcga ctcggcttta
     3121 ttcaataccc gagaaggacg tgccggaaag gtgcataact tcatgctggg cttgaatctc
     3181 aacacatcat atccactgtc tcccctgaga gacttcagct ctcaggattc cttcgatgac
     3241 gagctcgacg cagcggtagc agatccagat gaatttgaac gaatatatga accactggat
     3301 gtcaaaagta agaagattca tgtggtagat agtgggctca catttaacct gccgtatccc
     3361 ttgattctgc gacctcagag aggtgtggat cttatcatct cctttgactt ttctgcaagg
     3421 ccgagtgaca ccagtccccc tttcaaggaa cttctgcttg cagagaagtg ggcgaaaatg
     3481 aacaagcttc cctttccaaa gatcgatcct tatgtgtttg atcgggaagg attaaaggaa
     3541 tgctatgttt ttaaacctaa gaatcctgat gtggagaagg attgcccaac cattatccac
     3601 tttgttctgg ccaacatcaa cttcagaaag tacaaggccc caggtgttct aagggaaacc
     3661 aaagaagaga aagaaatcgc tgactttgac atttttgatg accccgaatc gccattttca
     3721 accttcaact ttcagtatcc caatcaagca ttcaaaaggc tgcatgatct gatgtacttc
     3781 aacacactga acaacattga tgtgataaag gatgccattg ttgagagcat tgaatacaga
     3841 agacagaacc catctcgttg ctctgtttcc ctcagtaatg ttgaagcaag aaaattcttc
     3901 aataaggagt ttctaagtaa acccactgtg taaggtctag agggcccttc gaacaaaaac
     3961 tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt
     4021 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4801 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5041 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5101 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     5161 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa
     5221 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     5281 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     5341 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     5401 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     5461 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     5521 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     5581 gtgtgggagg gctaagcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     5641 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5701 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     5761 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5821 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5881 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     5941 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6001 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6061 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6121 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6181 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6241 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8041 catttccccg aaaagtgcca cctgacgtc
//
