LOCUS       V007620                 7345 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007620
VERSION     V007620
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7345)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7345)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        351..1529
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1546..1564
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1639..1677
                     /label="E-tag"
                     /note="E-tag"
     CDS             1687..3189
                     /gene="Traf2"
                     /label="TNF receptor-associated factor 2"
                     /note="TNF receptor-associated factor 2 from Mus musculus.
                     Accession#: P39429"
     CDS             3227..3256
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3272..3289
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3318..3542
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3588..4016
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4030..4359
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4407..4454
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4473..4868
                     /label="BSD"
                     /note="blasticidin S deaminase"
     polyA_signal    5029..5162
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5199..5215)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5223..5239)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5247..5277)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5292..5313)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5590..6178)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6352..7209)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7210..7314)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
     1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg
     1681 gccgctatgg ctgcagccag tgtgacttcc cctggctccc tagaactgct acagcctggc
     1741 ttctccaaga ccctcctggg gaccaggtta gaagccaagt acctctgttc agcctgcaaa
     1801 aacatcctgc ggaggccttt ccaggcccag tgtgggcacc gctactgctc cttctgcctg
     1861 accagcatcc tcagctctgg gccccagaac tgtgctgcct gtgtctatga aggcctgtat
     1921 gaagaaggca tttctatttt agagagtagt tcggcctttc cagataacgc tgcccgcaga
     1981 gaggtggaga gcctgccagc tgtctgtccc aatgatggat gcacttggaa ggggaccttg
     2041 aaagaatacg agagctgcca cgaaggactt tgcccattcc tgctgacgga gtgtcctgca
     2101 tgtaaaggcc tggtccgcct cagcgagaag gagcaccaca ctgagcagga atgccccaaa
     2161 aggagcctga gctgccagca ctgcagagca ccctgtagcc acgtggacct ggaggtacac
     2221 tatgaggtct gccccaagtt tcccttaacc tgtgatggct gtggcaagaa gaagatccct
     2281 cgggagacgt ttcaggacca tgttagagca tgcagcaaat gccgggttct ctgcagattc
     2341 cacaccgttg gctgttcaga gatggtggag actgagaacc tgcaggatca tgagctgcag
     2401 cggctacggg aacacctagc cctactgctg agctcattct tggaggccca agcctctcca
     2461 ggaaccttga accaggtggg gccagagcta ctccagcggt gccagatttt ggagcagaag
     2521 atagcaacct ttgagaacat tgtctgcgtc ttgaaccgtg aagtagagag ggtagcagtg
     2581 actgcagagg cttgtagccg gcagcaccgg ctagaccagg acaagattga ggccctgagt
     2641 aacaaggtgc aacagctgga gaggagcatc ggcctcaagg acctggccat ggctgacctg
     2701 gagcagaagg tctccgagtt ggaagtatcc acctatgatg gggtcttcat ctggaagatc
     2761 tctgacttca ccagaaagcg tcaggaagcc gtagctggcc ggacaccagc tatcttctcc
     2821 ccagccttct acacaagcag atatggctac aagatgtgtc tacgagtcta cttgaatggc
     2881 gacggcactg ggcggggaac tcatctgtct ctcttcttcg tggtgatgaa aggccccaat
     2941 gatgctctgt tgcagtggcc ttttaatcag aaggtaacat tgatgttgct ggaccataac
     3001 aaccgggagc atgtgatcga cgcattcagg cccgatgtaa cctcgtcctc cttccagagg
     3061 cctgtcagtg acatgaacat cgccagtggc tgccccctct tctgccctgt gtccaagatg
     3121 gaggccaaga attcctatgt gcgggatgat gcgatcttca tcaaagctat tgtggaccta
     3181 acaggactct ctagataagc ggccgctcga gtctagaggg cccttcgaac aaaaactcat
     3241 ctcagaagag gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac
     3301 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3361 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3421 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3481 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3541 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3601 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3661 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3721 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3781 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3841 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3901 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3961 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4021 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4081 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4141 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4201 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4261 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4321 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4381 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     4441 tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat
     4501 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     4561 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     4621 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     4681 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     4741 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     4801 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     4861 gggagggcta agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     4921 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     4981 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     5041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5161 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5221 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5281 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5341 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5401 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5521 ggggataacg caggaaagaa catgtgagca aaaggccagg aaccgtaaaa aggccgcgtt
     5581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7321 tccccgaaaa gtgccacctg acgtc
//
