LOCUS       V007629                 7628 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007629
VERSION     V007629
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..1651
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1668..1686
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2029..3051
                     /gene="OPG199"
                     /label="Serine proteinase inhibitor 2"
                     /note="Serine proteinase inhibitor 2 from Cowpox virus
                     (strain Brighton Red). Accession#: P07385"
     CDS             3259..3300
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3310..3327
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3356..3580
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3626..4054
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4068..4397
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4464..5255
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5434..5567
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5604..5620)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5628..5644)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5652..5682)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5697..5718)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5995..6583)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6757..7614)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(7615..7628,1..91))
                     /label="AmpR promoter"
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1741 tgtggtggaa ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga
     1801 atataaaaaa tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct
     1861 ggaattagta tacacgctag attattctca aactcctaat tatgacagac tacgtagact
     1921 gtttatacaa gattgaaaat atatttcttt ttattgagtg gtggtagtta cggatatcta
     1981 atattaatat tagactatct ctatcgtcac acaacaaaat cgattgccat ggatatcttc
     2041 agggaaatcg catcttctat gaaaggagag aatgtattca tttctccacc gtcaatctcg
     2101 tcagtattga caatactgta ttatggagct aatggatcca ctgctgaaca gctatcaaaa
     2161 tatgtagaaa aggaggcgga caagaataag gatgatatct cattcaagtc catgaataaa
     2221 gtatatgggc gatattctgc agtgtttaaa gattcctttt tgagaaaaat tggagataat
     2281 ttccaaactg ttgacttcac tgattgtcgc actgtagatg cgatcaacaa gtgtgttgat
     2341 atcttcactg aggggaaaat taatccacta ttggatgaac cattgtctcc agatacctgt
     2401 ctcctagcaa ttagtgccgt atactttaaa gcaaaatggt tgatgccatt tgaaaaggaa
     2461 tttaccagtg attatccctt ttacgtatct ccaacggaaa tggtagatgt aagtatgatg
     2521 tctatgtacg gcgaggcatt taatcacgca tctgtaaaag aatcattcgg caacttttca
     2581 atcatagaac tgccatatgt tggagatact agtatggtgg taattcttcc agacaatatt
     2641 gatggactag aatccataga acaaaatcta acagatacaa attttaagaa atggtgtgac
     2701 tctatggatg ctatgtttat cgatgtgcac attcccaagt ttaaggtaac aggctcgtat
     2761 aatctggtgg atgcgctagt aaagttggga ctgacagagg tgttcggttc aactggagat
     2821 tatagcaata tgtgtaattc agatgtgagt gtcgacgcta tgatccacaa aacgtatata
     2881 gatgtcaatg aagagtatcg cgaagcggct gcagcaactt gtgcgctggt ggcagactgt
     2941 gcatcaacag ttacaaatga gttctgtgca gatcatccgt tcatctatgt gattaggcat
     3001 gtcgatggca aaattctttt cgttggtaga tattgctctc caacaactaa ttaaatcaca
     3061 ttcttaatat tagaatatta gaatattata tagttaagat ttttactaat tggttaacca
     3121 tttttttaaa aaaatagaaa aaaaacatgt tatattagcg agggtcgtta ttcttccaat
     3181 tgcaattggt aagatgacgg ggaattctgc agatatccag cacagtggcg gccgctcgag
     3241 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     3301 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     3361 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3421 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3481 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3541 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3601 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3841 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3961 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4021 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4081 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4141 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4201 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4261 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4321 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4381 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4441 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4981 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa
     5281 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5341 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5401 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5581 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5641 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5701 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5761 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5941 aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7621 cctttttc
//
