LOCUS       V037448                 7797 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037448
VERSION     V037448
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        123..1301
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             1352..4501
                     /label="Tlr7(NM_001290755)"
                     /note="Tlr7(NM_001290755)"
                     /gene="Tlr7"
     polyA_signal    4638..4759
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4766..5221)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5248..5352
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5354..5711
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5746..6540
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6772..6819
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7148..7736
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
      961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct
     1321 agcgtttaaa cttaagcttg gtaccgccac catggtgttt tcgatgtgga cacggaagag
     1381 acaaattttg atctttttaa atatgctctt agtttctaga gtctttgggt ttcgatggtt
     1441 tcctaaaact ctaccttgtg aagttaaagt aaatatccca gaggcccatg tgatcgtgga
     1501 ctgcacagac aagcatttga cagaaatccc tgagggcatt cccactaaca ccaccaatct
     1561 tacccttacc atcaaccaca taccaagcat ctctccagat tccttccgta ggctgaacca
     1621 tctggaagaa atcgatttaa gatgcaattg tgtacctgtt ctactggggt ccaaagccaa
     1681 tgtgtgtacc aagaggctgc agattagacc tggaagcttt agtggactct ctgacttaaa
     1741 agccctttac ctggatggaa accaacttct ggagatacca caggatctgc catccagctt
     1801 acatcttctg agccttgagg ctaacaacat cttctccatc acgaaggaga atctaacaga
     1861 actggtcaac attgaaacac tctacctggg tcaaaactgt tattatcgaa atccttgcaa
     1921 tgtttcctat tctattgaaa aagatgcttt cctagttatg agaaatttga aggttctctc
     1981 actaaaagat aacaatgtca cagctgtccc caccactttg ccacctaatt tactagagct
     2041 ctatctttat aacaatatca ttaagaaaat ccaagaaaat gattttaata acctcaatga
     2101 gttgcaagtt cttgacctaa gtggaaattg ccctcgatgt tataatgtcc catatccgtg
     2161 tacaccgtgt gaaaataatt cccccttaca gatccatgac aatgctttca attcattgac
     2221 agaattaaaa gttttacgtt tacacagtaa ttctcttcag catgtgcccc caacatggtt
     2281 taaaaacatg agaaacctcc aggaactaga cctctcccaa aactacttgg ccagagaaat
     2341 tgaggaggcc aaatttttgc attttcttcc caaccttgtt gagttggatt tttctttcaa
     2401 ttatgagctg caggtctacc atgcatctat aactttacca cattcactct cttcattgga
     2461 aaacttgaaa attctgcgtg tcaaggggta tgtctttaaa gagctgaaaa actccagtct
     2521 ttctgtattg cacaagcttc ccaggctgga agttcttgac cttggcacta acttcataaa
     2581 aattgctgac ctcaacatat tcaaacattt tgaaaacctc aaactcatag acctttcagt
     2641 gaataagata tctccttcag aagagtcaag agaagttggc ttttgtccta atgctcaaac
     2701 ttctgtagac cgtcatgggc cccaggtcct tgaggcctta cactatttcc gatacgatga
     2761 atatgcacgg agctgcaggt tcaaaaacaa agagccacct tctttcttgc ctttgaatgc
     2821 agactgccac atatatgggc agaccttaga cttaagtaga aataacatat tttttattaa
     2881 accttctgat tttcagcatc tttcattcct caaatgcctc aacttatcag gaaacaccat
     2941 tggccaaact cttaatggca gtgaactctg gccgttgaga gagttgcggt acttagactt
     3001 ctccaacaac cggcttgatt tactctactc aacagccttt gaagagctcc agagtcttga
     3061 agttctggat ctaagtagta acagccacta ttttcaagca gaaggaatta ctcacatgct
     3121 aaactttacc aagaaattac ggcttctgga caaactcatg atgaatgata atgacatctc
     3181 tacttcggcc agcaggacca tggaaagtga ctctcttcga attctggagt tcagaggcaa
     3241 ccatttagat gttctatgga gagccggtga taacagatac ttggacttct tcaagaattt
     3301 gttcaattta gaggtattag atatctccag aaattccctg aattccttgc ctcctgaggt
     3361 ttttgagggt atgccgccaa atctaaagaa tctctccttg gccaaaaatg ggctcaaatc
     3421 tttcttttgg gacagactcc agttactgaa gcatttggaa attttggacc tcagccataa
     3481 ccagctgaca aaagtacctg agagattggc caactgttcc aaaagtctca caacactgat
     3541 tcttaagcat aatcaaatca ggcaattgac aaaatatttt ctagaagatg ctttgcaatt
     3601 gcgctatcta gacatcagtt caaataaaat ccaggtcatt cagaagacta gcttcccaga
     3661 aaatgtcctc aacaatctgg agatgttggt tttacatcac aatcgctttc tttgcaactg
     3721 tgatgctgtg tggtttgtct ggtgggttaa ccatacagat gttactattc catacctggc
     3781 cactgatgtg acttgtgtag gtccaggagc acacaaaggt caaagtgtca tatcccttga
     3841 tctgtatacg tgtgagttag atctcacaaa cctgattctg ttctcagttt ccatatcatc
     3901 agtcctcttt cttatggtag ttatgacaac aagtcacctc tttttctggg atatgtggta
     3961 catttattat ttttggaaag caaagataaa ggggtatcag catctgcaat ccatggagtc
     4021 ttgttatgat gcttttattg tgtatgacac taaaaactca gctgtgacag aatgggtttt
     4081 gcaggagctg gtggcaaaat tggaagatcc aagagaaaaa cacttcaatt tgtgtctaga
     4141 agaaagagac tggctaccag gacagccagt tctagaaaac ctttcccaga gcatacagct
     4201 cagcaaaaag acagtgtttg tgatgacaca gaaatatgct aagactgaga gttttaagat
     4261 ggcattttat ttgtctcatc agaggctcct ggatgaaaaa gtggatgtga ttatcttgat
     4321 attcttggaa aagcctcttc agaagtctaa gtttcttcag ctcaggaaga gactctgcag
     4381 gagctctgtc cttgagtggc ctgcaaatcc acaggctcac ccatacttct ggcagtgcct
     4441 gaaaaatgcc ctgaccacag acaatcatgt ggcttatagt caaatgttca aggaaacagt
     4501 ctagggatcc tcagacgcgg ccgcgactct agatcataat cagccatacc acatttgtag
     4561 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4621 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4681 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4741 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     4801 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4861 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4921 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4981 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5041 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5101 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5161 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5221 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5281 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5341 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5461 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5521 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5581 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5641 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5701 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5761 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5821 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5881 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     5941 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6001 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6061 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6121 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6181 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6241 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6301 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6361 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6421 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6481 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6541 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6601 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6661 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     6721 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     6781 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     6841 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     6901 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     6961 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7021 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7081 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7261 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7501 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
