LOCUS       V031813                 6098 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031813
VERSION     V031813
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        123..1301
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             1364..2719
                     /label="IDH2(NM_002168)"
                     /note="IDH2(NM_002168)"
                     /gene="IDH2"
     CDS             2726..2815
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    2939..3060
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3067..3522)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3549..3653
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3655..4012
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4047..4841
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5073..5120
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5449..6037
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
      961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct
     1321 agcgtttaaa cttaagcttg gtaccgagct cggatccgcc accatggccg gctacctgcg
     1381 ggtcgtgcgc tcgctctgca gagcctcagg ctcgcggccg gcctgggcgc cggcggccct
     1441 gacagccccc acctcgcaag agcagccgcg gcgccactat gccgacaaaa ggatcaaggt
     1501 ggcgaagccc gtggtggaga tggatggtga tgagatgacc cgtattatct ggcagttcat
     1561 caaggagaag ctcatcctgc cccacgtgga catccagcta aagtattttg acctcgggct
     1621 cccaaaccgt gaccagactg atgaccaggt caccattgac tctgcactgg ccacccagaa
     1681 gtacagtgtg gctgtcaagt gtgccaccat cacccctgat gaggcccgtg tggaagagtt
     1741 caagctgaag aagatgtgga aaagtcccaa tggaactatc cggaacatcc tgggggggac
     1801 tgtcttccgg gagcccatca tctgcaaaaa catcccacgc ctagtccctg gctggaccaa
     1861 gcccatcacc attggcaggc acgcccatgg cgaccagtac aaggccacag actttgtggc
     1921 agaccgggcc ggcactttca aaatggtctt caccccaaaa gatggcagtg gtgtcaagga
     1981 gtgggaagtg tacaacttcc ccgcaggcgg cgtgggcatg ggcatgtaca acaccgacga
     2041 gtccatctca ggttttgcgc acagctgctt ccagtatgcc atccagaaga aatggccgct
     2101 gtacatgagc accaagaaca ccatactgaa agcctacgat gggcgtttca aggacatctt
     2161 ccaggagatc tttgacaagc actataagac cgacttcgac aagaataaga tctggtatga
     2221 gcaccggctc attgatgaca tggtggctca ggtcctcaag tcttcgggtg gctttgtgtg
     2281 ggcctgcaag aactatgacg gagatgtgca gtcagacatc ctggcccagg gctttggctc
     2341 ccttggcctg atgacgtccg tcctggtctg ccctgatggg aagacgattg aggctgaggc
     2401 cgctcatggg accgtcaccc gccactatcg ggagcaccag aagggccggc ccaccagcac
     2461 caaccccatc gccagcatct ttgcctggac acgtggcctg gagcaccggg ggaagctgga
     2521 tgggaaccaa gacctcatca ggtttgccca gatgctggag aaggtgtgcg tggagacggt
     2581 ggagagtgga gccatgacca aggacctggc gggctgcatt cacggcctca gcaatgtgaa
     2641 gctgaacgag cacttcctga acaccacgga cttcctcgac accatcaaga gcaacctgga
     2701 cagagccctg ggcaggcagc tcgaggaaca aaaactaata tcagaggaag atttggaaca
     2761 gaaactaatc agtgaagaag atttagagca aaagttaatt tccgaggagg acttatagcg
     2821 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     2881 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     2941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3061 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     3121 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3181 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3241 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3301 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3361 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3421 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3481 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     3541 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3601 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     3661 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3721 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3781 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3841 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3901 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3961 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     4021 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4081 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4141 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4201 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     4261 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4321 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4381 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4441 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4501 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4561 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4621 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4681 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4741 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4801 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     4861 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4921 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4981 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     5041 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     5101 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     5161 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     5221 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     5281 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5341 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5401 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5581 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6061 acggttcctg gccttttgct ggccttttgc tcacatgt
//
