LOCUS V042760 7872 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042760 VERSION V042760 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 123..1301 /note="strong constitutive promoter for human elongation factor EF-1α" /label="EF-1α promoter" CDS 1352..3829 /label="HIF1A(NM_001530)" /note="HIF1A(NM_001530)" /gene="HIF1A" CDS 3872..4591 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 4713..4834 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4841..5296) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5323..5427 /gene="bla" /label="AmpR promoter" promoter 5429..5786 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5821..6615 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6847..6894 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 7223..7811 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt 61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa 121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct 1321 agcgtttaaa cttaagcttg gtaccgccac catggagggc gccggcggcg cgaacgacaa 1381 gaaaaagata agttctgaac gtcgaaaaga aaagtctcga gatgcagcca gatctcggcg 1441 aagtaaagaa tctgaagttt tttatgagct tgctcatcag ttgccacttc cacataatgt 1501 gagttcgcat cttgataagg cctctgtgat gaggcttacc atcagctatt tgcgtgtgag 1561 gaaacttctg gatgctggtg atttggatat tgaagatgac atgaaagcac agatgaattg 1621 cttttatttg aaagccttgg atggttttgt tatggttctc acagatgatg gtgacatgat 1681 ttacatttct gataatgtga acaaatacat gggattaact cagtttgaac taactggaca 1741 cagtgtgttt gattttactc atccatgtga ccatgaggaa atgagagaaa tgcttacaca 1801 cagaaatggc cttgtgaaaa agggtaaaga acaaaacaca cagcgaagct tttttctcag 1861 aatgaagtgt accctaacta gccgaggaag aactatgaac ataaagtctg caacatggaa 1921 ggtattgcac tgcacaggcc acattcacgt atatgatacc aacagtaacc aacctcagtg 1981 tgggtataag aaaccaccta tgacctgctt ggtgctgatt tgtgaaccca ttcctcaccc 2041 atcaaatatt gaaattcctt tagatagcaa gactttcctc agtcgacaca gcctggatat 2101 gaaattttct tattgtgatg aaagaattac cgaattgatg ggatatgagc cagaagaact 2161 tttaggccgc tcaatttatg aatattatca tgctttggac tctgatcatc tgaccaaaac 2221 tcatcatgat atgtttacta aaggacaagt caccacagga cagtacagga tgcttgccaa 2281 aagaggtgga tatgtctggg ttgaaactca agcaactgtc atatataaca ccaagaattc 2341 tcaaccacag tgcattgtat gtgtgaatta cgttgtgagt ggtattattc agcacgactt 2401 gattttctcc cttcaacaaa cagaatgtgt ccttaaaccg gttgaatctt cagatatgaa 2461 aatgactcag ctattcacca aagttgaatc agaagataca agtagcctct ttgacaaact 2521 taagaaggaa cctgatgctt taactttgct ggccccagcc gctggagaca caatcatatc 2581 tttagatttt ggcagcaacg acacagaaac tgatgaccag caacttgagg aagtaccatt 2641 atataatgat gtaatgctcc cctcacccaa cgaaaaatta cagaatataa atttggcaat 2701 gtctccatta cccaccgctg aaacgccaaa gccacttcga agtagtgctg accctgcact 2761 caatcaagaa gttgcattaa aattagaacc aaatccagag tcactggaac tttcttttac 2821 catgccccag attcaggatc agacacctag tccttccgat ggaagcacta gacaaagttc 2881 acctgagcct aatagtccca gtgaatattg tttttatgtg gatagtgata tggtcaatga 2941 attcaagttg gaattggtag aaaaactttt tgctgaagac acagaagcaa agaacccatt 3001 ttctactcag gacacagatt tagacttgga gatgttagct ccctatatcc caatggatga 3061 tgacttccag ttacgttcct tcgatcagtt gtcaccatta gaaagcagtt ccgcaagccc 3121 tgaaagcgca agtcctcaaa gcacagttac agtattccag cagactcaaa tacaagaacc 3181 tactgctaat gccaccacta ccactgccac cactgatgaa ttaaaaacag tgacaaaaga 3241 ccgtatggaa gacattaaaa tattgattgc atctccatct cctacccaca tacataaaga 3301 aactactagt gccacatcat caccatatag agatactcaa agtcggacag cctcaccaaa 3361 cagagcagga aaaggagtca tagaacagac agaaaaatct catccaagaa gccctaacgt 3421 gttatctgtc gctttgagtc aaagaactac agttcctgag gaagaactaa atccaaagat 3481 actagctttg cagaatgctc agagaaagcg aaaaatggaa catgatggtt cactttttca 3541 agcagtagga attggaacat tattacagca gccagacgat catgcagcta ctacatcact 3601 ttcttggaaa cgtgtaaaag gatgcaaatc tagtgaacag aatggaatgg agcaaaagac 3661 aattatttta ataccctctg atttagcatg tagactgctg gggcaatcaa tggatgaaag 3721 tggattacca cagctgacca gttatgattg tgaagttaat gctcctatac aaggcagcag 3781 aaacctactg cagggtgaag aattactcag agctttggat caagttaacg gtggaggcgg 3841 atcaggaggc ggtggatctg gatccgccac catggtgagc aagggcgagg agctgttcac 3901 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3961 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 4021 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 4081 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 4141 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 4201 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 4261 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 4321 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 4381 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4441 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 4501 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4561 cactctcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc 4621 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 4681 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 4741 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4801 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt 4861 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 4921 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 4981 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 5041 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 5101 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 5161 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 5221 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 5281 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 5341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5401 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 5461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 5521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 5581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5761 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 5821 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 5881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 5941 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 6001 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 6241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 6301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 6361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 6421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 6481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 6541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 6601 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 6661 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 6721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 6781 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 6841 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 6901 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 6961 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 7021 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 7081 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 7141 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 7201 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 7261 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 7321 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 7381 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 7441 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 7501 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7561 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 7621 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 7681 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 7741 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 7801 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 7861 ttgctcacat gt //