LOCUS       V007630                 8089 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007630
VERSION     V007630
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8089)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8089)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..1651
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1668..1686
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1731..3698
                     /gene="Ripk1"
                     /label="Receptor-interacting serine/threonine-protein
                     kinase 1"
                     /note="Receptor-interacting serine/threonine-protein kinase
                     1 from Mus musculus. Accession#: Q60855"
     CDS             3720..3761
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3771..3788
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3817..4041
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4087..4515
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4529..4858
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4925..5716
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5895..6028
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6065..6081)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6089..6105)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6113..6143)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6158..6179)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6456..7044)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7218..8075)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(8076..8089,1..91))
                     /label="AmpR promoter"
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc atgcaaccag
     1741 acatgtcctt ggacaatatt aagatggcat ccagtgacct gctggagaag acagacctag
     1801 acagcggagg cttcgggaag gtgtccttgt gttaccacag aagccatgga tttgtcatcc
     1861 tgaaaaaagt atacacaggg cccaaccgcg ctgagtacaa tgaggttctc ttggaagagg
     1921 ggaagatgat gcacagactg agacacagtc gagtggtgaa gctactgggc atcatcatag
     1981 aagaagggaa ctattcgctg gtgatggagt acatggagaa gggcaacctg atgcacgtgc
     2041 taaagaccca gatagatgtc ccactttcat tgaaaggaag gataatcgtg gaggccatag
     2101 aaggcatgtg ctacttacat gacaaaggtg tgatacacaa ggacctgaag cctgagaata
     2161 tcctcgttga tcgtgacttt cacattaaga tagccgatct tggtgtggct tcctttaaga
     2221 catggagcaa actgactaag gagaaagaca acaagcagaa agaagtgagc agcaccacta
     2281 agaagaacaa tggtggtacc ctttactaca tggcacccga acacctgaat gacatcaatg
     2341 caaagcccac ggagaagtcg gacgtgtaca gctttggcat tgtcctttgg gcaatatttg
     2401 caaaaaagga gccctatgag aatgtcatct gtactgagca gttcgtgatc tgcataaaat
     2461 ctgggaacag gccaaatgta gaggaaatcc ttgagtactg tccaagggag atcatcagcc
     2521 tcatggagcg gtgctggcag gcgatcccag aagacaggcc aacatttctt ggcattgaag
     2581 aagaatttag gcctttttac ttaagtcatt ttgaagaata tgtagaagag gatgtggcaa
     2641 gtttaaagaa agagtatcca gatcaaagcc cagtgctgca gagaatgttt tcactgcagc
     2701 atgactgtgt acccttacct ccgagcaggt caaattcaga acaacctgga tcgctgcaca
     2761 gttcccaggg gctccagatg ggtcctgtgg aggagtcctg gttttcttcc tccccagagt
     2821 acccacagga cgagaatgat cgcagtgtgc aggctaagct gcaagaggaa gccagctatc
     2881 atgcttttgg aatatttgca gagaaacaga caaaaccgca gccaaggcag aatgaggctt
     2941 acaacagaga ggaggaaagg aaacgaaggg tctctcatga cccctttgca cagcagagag
     3001 ctcgtgagaa tattaagagt gcaggagcaa gaggtcattc tgatcccagc acaacgagtc
     3061 gtggaattgc agtgcaacag ctgtcatggc cagccaccca aacagtttgg aacaatggat
     3121 tgtataatca gcatggattt ggaactacag gtacaggagt ttggtatccg ccaaatctaa
     3181 gccaaatgta tagtacttat aaaactccag tgccagagac caacataccg ggaagcacac
     3241 ccaccatgcc atacttctct gggccagtag cagatgacct cataaaatat actatattca
     3301 atagttctgg tattcagatt ggaaaccaca attatatgga tgttggactg aattcacaac
     3361 caccaaacaa tacttgcaaa gaagagtcga cttccagaca ccaagccatc tttgataaca
     3421 ccactagtct gactgatgaa cacctgaacc ctatcaggga aaacctggga aggcagtgga
     3481 aaaactgtgc ccgcaagctg ggcttcactg agtctcagat cgatgaaatc gaccatgact
     3541 atgaaagaga tggactgaaa gagaaagttt accaaatgct tcagaagtgg ctgatgcggg
     3601 aaggcaccaa aggggccaca gtgggaaagt tggcccaggc acttcaccaa tgttgcagga
     3661 tagacctgct gaaccacttg attcgtgcca gccagagcta atctagaggg cccttcgaag
     3721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     3781 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3841 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3901 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3961 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4021 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4081 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4141 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4201 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4261 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4321 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4381 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4441 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4501 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4861 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     4921 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4981 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5041 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5101 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5161 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5221 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5281 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5341 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5401 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5461 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5521 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5581 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5641 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5701 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
     5761 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5821 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5881 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6001 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6061 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttc
//
