LOCUS       V007632                 7966 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007632
VERSION     V007632
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7966)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7966)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..1651
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1668..1686
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1784..1813
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2057..3547
                     /gene="XIAP"
                     /label="E3 ubiquitin-protein ligase XIAP"
                     /note="E3 ubiquitin-protein ligase XIAP from Homo sapiens.
                     Accession#: P98170"
     CDS             3597..3638
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3648..3665
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3694..3918
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3964..4392
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4406..4735
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4802..5593
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5772..5905
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5942..5958)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5966..5982)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5990..6020)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6035..6056)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6333..6921)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7095..7952)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(7953..7966,1..91))
                     /label="AmpR promoter"
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1741 tgtggtggaa ttctgcagat agcttcggta tcgataagct atggagcaaa agctcatttc
     1801 tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat
     1861 ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga
     1921 agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga
     1981 gagcttgggc gacctcacca tggagcaaaa gctcatttct gaagaggact tgaattcctg
     2041 cagcccgggg ggatccatga cttttaacag ttttgaagga tctaaaactt gtgtacctgc
     2101 agacatcaat aaggaagaag aatttgtaga agagtttaat agattaaaaa cttttgctaa
     2161 ttttccaagt ggtagtcctg tttcagcatc aacactggca cgagcagggt ttctttatac
     2221 tggtgaagga gataccgtgc ggtgctttag ttgtcatgca gctgtagata gatggcaata
     2281 tggagactca gcagttggaa gacacaggaa agtatcccca aattgcagat ttatcaacgg
     2341 cttttatctt gaaaatagtg ccacgcagtc tacaaattct ggtatccaga atggtcagta
     2401 caaagttgaa aactatctgg gaagcagaga tcattttgcc ttagacaggc catctgagac
     2461 acatgcagac tatcttttga gaactgggca ggttgtagat atatcagaca ccatataccc
     2521 gaggaaccct gccatgtatt gtgaagaagc tagattaaag tcctttcaga actggccaga
     2581 ctatgctcac ctaaccccaa gagagttagc aagtgctgga ctctactaca caggtattgg
     2641 tgaccaagtg cagtgctttt gttgtggtgg aaaactgaaa aattgggaac cttgtgatcg
     2701 tgcctggtca gaacacaggc gacactttcc taattgcttc tttgttttgg gccggaatct
     2761 taatattcga agtgaatctg atgctgtgag ttctgatagg aatttcccaa attcaacaaa
     2821 tcttccaaga aatccatcca tggcagatta tgaagcacgg atctttactt ttgggacatg
     2881 gatatactca gttaacaagg agcagcttgc aagagctgga ttttatgctt taggtgaagg
     2941 tgataaagta aagtgctttc actgtggagg agggctaact gattggaagc ccagtgaaga
     3001 cccttgggaa caacatgcta aatggtatcc agggtgcaaa tatctgttag aacagaaggg
     3061 acaagaatat ataaacaata ttcatttaac tcattcactt gaggagtgtc tggtaagaac
     3121 tactgagaaa acaccatcac taactagaag aattgatgat accatcttcc aaaatcctat
     3181 ggtacaagaa gctatacgaa tggggttcag tttcaaggac attaagaaaa taatggagga
     3241 aaaaattcag atatctggga gcaactataa atcacttgag gttctggttg cagatctagt
     3301 gaatgctcag aaagacagta tgcaagatga gtcaagtcag acttcattac agaaagagat
     3361 tagtactgaa gagcagctaa ggcgcctgca agaggagaag ctttgcaaaa tctgtatgga
     3421 tagaaatatt gctatcgttt ttgttccttg tggacatcta gtcacttgta aacaatgtgc
     3481 tgaagcagtt gacaagtgtc ccatgtgcta cacagtcatt actttcaagc aaaaaatttt
     3541 tatgtcttaa tctaactcta tagtagctcg agcatgcatc tagagggccc ttcgaaggta
     3601 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     3661 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3721 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3781 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3841 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3901 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     3961 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4021 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4081 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4141 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4201 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4261 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4321 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4381 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4561 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4741 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4801 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4861 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4921 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4981 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5041 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5101 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5161 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5221 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5281 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5341 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5401 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5461 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5521 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5581 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     5641 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5701 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5761 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5881 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttc
//
