LOCUS V019395 9173 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019395 VERSION V019395 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 50..1228 /note="strong constitutive promoter for human elongation factor EF-1α" /label="EF-1α promoter" CDS 1259..3847 /label="ADAM15(NM_207197)" /note="ADAM15(NM_207197)" /gene="ADAM15" CDS 3860..3925 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3949..4537 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4603..4651 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4853..5281 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5295..5624 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5691..6485 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6661..6782 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6831..6847) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6855..6871 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6879..6909) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6924..6945 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7233..7821) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7992..8852) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8853..8957) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg gctccggtgc 61 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 121 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 181 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 241 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc 301 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg 361 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag 421 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg 481 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc 541 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct 601 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga 661 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc 721 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc 781 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc 841 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc 901 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt 961 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag 1021 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca 1081 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga 1141 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag 1201 tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtactggcta gcgccaccat 1261 gcggctggcg ctgctctggg ccctggggct cctgggcgcg ggcagccctc tgccttcctg 1321 gccgctccca aatataggtg gcactgagga gcagcaggca gagtcagaga aggccccgag 1381 ggagcccttg gagccccagg tccttcagga cgatctccca attagcctca aaaaggtgct 1441 tcagaccagt ctgcctgagc ccctgaggat caagttggag ctggacggtg acagtcatat 1501 cctggagctg ctacagaata gggagttggt cccaggccgc ccaaccctgg tgtggtacca 1561 gcccgatggc actcgggtgg tcagtgaggg acacactttg gagaactgct gctaccaggg 1621 aagagtgcgg ggatatgcag gctcctgggt gtccatctgc acctgctctg ggctcagagg 1681 cttggtggtc ctgaccccag agagaagcta taccctggag caggggcctg gggaccttca 1741 gggtcctccc attatttcgc gaatccaaga tctccacctg ccaggccaca cctgtgccct 1801 gagctggcgg gaatctgtac acactcagaa gccaccagag caccccctgg gacagcgcca 1861 cattcgccgg aggcgggatg tggtaacaga gaccaagact gtggagttgg tgattgtggc 1921 tgatcactcg gaggcccaga aataccggga cttccagcac ctgctaaacc gcacactgga 1981 agtggccctc ttgctggaca cattcttccg gcccctgaat gtacgagtgg cactagtggg 2041 cctggaggcc tggacccagc gtgacctggt ggagatcagc ccaaacccag ctgtcaccct 2101 cgaaaacttc ctccactggc gcagggcaca tttgctgcct cgattgcccc atgacagtgc 2161 ccagctggtg actggtactt cattctctgg gcctacggtg ggcatggcca ttcagaactc 2221 catctgttct cctgacttct caggaggtgt gaacatggac cactccacca gcatcctggg 2281 agtcgcctcc tccatagccc atgagttggg ccacagcctg ggcctggacc atgatttgcc 2341 tgggaatagc tgcccctgtc caggtccagc cccagccaag acctgcatca tggaggcctc 2401 cacagacttc ctaccaggcc tgaacttcag caactgcagc cgacgggccc tggagaaagc 2461 cctcctggat ggaatgggca gctgcctctt cgaacggctg cctagcctac cccctatggc 2521 tgctttctgc ggaaatatgt ttgtggagcc gggcgagcag tgtgactgtg gcttcctgga 2581 tgactgcgtc gatccctgct gtgattcttt gacctgccag ctgaggccag gtgcacagtg 2641 tgcatctgac ggaccctgtt gtcaaaattg ccagctgcgc ccgtctggct ggcagtgtcg 2701 tcctaccaga ggggattgtg acttgcctga attctgccca ggagacagct cccagtgtcc 2761 ccctgatgtc agcctagggg atggcgagcc ctgcgctggc gggcaagctg tgtgcatgca 2821 cgggcgttgt gcctcctatg cccagcagtg ccagtcactt tggggacctg gagcccagcc 2881 cgctgcgcca ctttgcctcc agacagctaa tactcgggga aatgcttttg ggagctgtgg 2941 gcgcaacccc agtggcagtt atgtgtcctg cacccctaga gatgccattt gtgggcagct 3001 ccagtgccag acaggtagga cccagcctct gctgggctcc atccgggatc tactctggga 3061 gacaatagat gtgaatggga ctgagctgaa ctgcagctgg gtgcacctgg acctgggcag 3121 tgatgtggcc cagcccctcc tgactctgcc tggcacagcc tgtggccctg gcctggtgtg 3181 tatagaccat cgatgccagc gtgtggatct cctgggggca caggaatgtc gaagcaaatg 3241 ccatggacat ggggtctgtg acagcaacag gcactgctac tgtgaggagg gctgggcacc 3301 ccctgactgc accactcagc tcaaagcaac cagctccctg accacagggc tgctcctcag 3361 cctcctggtc ttattggtcc tggtgatgct tggtgccagc tactggtacc gtgcccgcct 3421 gcaccagcga ctctgccagc tcaagggacc cacctgccag tacagggcag cccaatctgg 3481 tccctctgaa cggccaggac ctccgcagag ggccctgctg gcacgaggca ctaagcaggc 3541 tagtgctctc agcttcccgg cccccccttc caggccgctg ccgcctgacc ctgtgtccaa 3601 gagactccag gctgagctgg ctgaccgacc caatccccct acccgccctc tgcccgctga 3661 cccggtggtg agaagcccga agtctcaggg gccagccaag cccccacccc caaggaagcc 3721 actgcctgcc gacccccagg gccggtgccc atcgggtgac ctgcccggcc caggggctgg 3781 aatcccgccc ctagtggtac cctccagacc agcgccaccg cctccgacag tgtcctcgct 3841 ctacctccgg ccgctcgagg actacaaaga ccatgacggt gattataaag atcatgacat 3901 cgactacaag gatgacgatg acaagtagtg agggcccgat atctcgacaa tcaacctctg 3961 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4021 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4081 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4141 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4201 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4261 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4321 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 4381 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 4441 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 4501 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg 4561 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 4621 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 4681 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 4741 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 4801 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc 4861 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4921 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 4981 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta 5041 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 5101 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 5161 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 5221 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5281 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 5341 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 5401 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 5461 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 5521 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 5581 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 5641 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 5701 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 5761 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 5821 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 5881 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 5941 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 6001 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 6061 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 6121 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 6181 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 6241 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 6301 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 6361 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 6421 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 6481 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca 6541 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 6601 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 6661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 6721 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6781 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 6841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6961 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 7021 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 7081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7141 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7201 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7261 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 7441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7681 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7801 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7861 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 7921 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 7981 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8041 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8101 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8161 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8221 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8281 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8341 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8401 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8461 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8521 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8581 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8641 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 8701 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 8761 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 8821 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 8881 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 8941 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 9001 gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 9061 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 9121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg //