LOCUS       V017342                 6046 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017342
VERSION     V017342
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2251..2461
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     promoter        2558..3736
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3768..4412
                     /gene="TIRAP"
                     /label="Toll/interleukin-1 receptor domain-containing
                     adapter protein"
                     /note="Toll/interleukin-1 receptor domain-containing
                     adapter protein from Homo sapiens. Accession#: P58753"
     CDS             4479..4490
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             4500..4523
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4524..4541
                     /label="6xHis"
                     /note="6xHis affinity tag"
     primer_bind     complement(5176..5192)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(5460..5840)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2461 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2521 aatggacctt ctaggtcttg aaaggagtgc ctcgtgaggc tccggtgccc gtcagtgggc
     2581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     2641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2761 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     2821 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     2881 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     2941 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     3001 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     3061 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     3121 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     3181 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     3241 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     3301 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3361 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3421 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3481 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3541 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3601 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     3661 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     3721 atttcaggtg tcgtgaggaa ttctctagag atccctcgac ctcgagtatg gcatcatcga
     3781 cctccctccc agctcctggc tctcggccta agaagcctct aggcaagatg gctgactggt
     3841 tcaggcagac cctgctgaag aagcccaaga agaggcccaa ctccccagaa agcacctcca
     3901 gcgatgcttc acagcctacc tcacaggaca acccactacc cccaagcctc agctcagtca
     3961 cgtctcccag cctgccaccc acacatgcga gtgacagtgg cagtagtcgc tggagcaaag
     4021 actatgacgt ctgcgtgtgc cacagtgagg aagacctggt ggccgcccag gacctggtct
     4081 cctacttgga aggcagcact gccagcctgc gctgcttcct gcaactccgg gatgcaaccc
     4141 caggcggcgc tatagtgtcc gagctgtgcc aggcactgag cagtagtcac tgccgggtgc
     4201 tgctcatcac gccgggcttc cttcaggacc cctggtgcaa gtaccagatg ctgcaggccc
     4261 tgaccgaggc tccaggggcc gagggctgca ccatccccct gctgtcgggc ctcagcagag
     4321 ctgcctaccc acctgagctc cgattcatgt actacgtcga tggcaggggc cctgatggtg
     4381 gctttcgtca agtcaaagaa gctgtcatgc gttgtaagct actacaggag ggagaagggg
     4441 aacgggattc agctacagta tctgatctac ttggatccat tgaaggaagg atcgtcaagg
     4501 actacaagga cgacgatgac aagcaccacc accaccacca ctgagcggcc gcgactctag
     4561 agtgagggtc cccacctggg acccttgaga gtatcaggtc tcccacgtgg gagacaagaa
     4621 atccctgttt aatatttaaa cagcagtgtt ccccatctgg gtccttgcac ccctcactct
     4681 ggcctcagcc gactgcacag cggcccctgc atccccttgg ctgtgaggcc cctggacaag
     4741 cagaggtggc cagagctggg aggcatggcc ctggggtccc acgaatttgc tggggaatct
     4801 cgtttttctt cttaagactt ttgggacatg gtttgactcc cgaacatcac cgacgcgtct
     4861 cctgtttttc tgggtggcct cgggacacct gccctgcccc cacgagggtc aggactgtga
     4921 ctctttttag ggccaggcag gtgcctggac atttgccttg ctggacgggg actggggatg
     4981 tgggagggag cagacaggag gaatcatgtc aggcctgtgt gtgaaaggaa gctccactgt
     5041 caccctccac ctcttcaccc cccactcacc agtgtcccct ccactgtcac attgtaactg
     5101 aacttcagga taataaagtg cttgcctcca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5161 aaaaaaaaag aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5221 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5281 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5341 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaaaacgt taatattttg
     5401 ttaaaattcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggttc
     5461 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt
     5521 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5581 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
     5641 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5701 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5761 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5821 tgcgccgcta cagggcgcgt actatggttg ctttgacgag catggtgcac tctcagtaca
     5881 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
