LOCUS       40924_16845        5679 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pEF-BOShFasRI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5679)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5679)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      207..587
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(918..1484)
                     /label=polyA
                     /note="polyA"
                     /regulatory_class="terminator"
     CDS             complement(1492..1929)
                     /codon_start=1
                     /note="unnamed protein product; hFasRI"
                     /protein_id="SJL87974.1"
                     /translation="MKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYIT
                     TIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDT
                     LIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV"
     promoter        complement(1944..3122)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             complement(3128..3197)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87975.1"
                     /translation="MDKVLNREESLQLMDLLGLERSA"
     promoter        complement(3219..3429)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     complement(3458..3474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3482..3498)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3506..3536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3551..3572)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3860..4448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4622..5479)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5480..5584)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
      601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
      661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
      901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
      961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
     1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
     1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
     1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
     1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
     1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
     1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
     1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
     1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag cgaccaagct
     1501 ttggatttca tttctgaagt ttgaattttc tgagtcacta gtaatgtcct tgaggatgat
     1561 agtctgaatt ttctctgcaa gagtacaaag attggctttt ttgagatctt taatcaatgt
     1621 gtcatacgct tctttctttc catgaagttg atgccaatta cgaagcagtt gaactttctg
     1681 ttctgctgtg tcttggacat tgtcattctt gatctcatct attttggctt cattgacacc
     1741 attctttcga acaaagcctt taacttgact tagtgtcatg actccagcaa tagtggtgat
     1801 atatttactc aagtcaacat cagataaatt tattgccact gtttcaggat ttaaggttgg
     1861 agattcatga gaaccttggt tttcctttct gtgctttctg catgttttct gtacttcctt
     1921 tctcttcacc atggtggaat tcctcacgac acctgaaatg gaagaaaaaa actttgaacc
     1981 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
     2041 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
     2101 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
     2161 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
     2221 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
     2281 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
     2341 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg
     2401 ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc
     2461 gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc
     2521 gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct
     2581 tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg
     2641 aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca
     2701 ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc
     2761 tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa
     2821 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac
     2881 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
     2941 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
     3001 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct
     3061 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag
     3121 cctcacgagg cactcctttc aagacctaga aggtccatta gctgcaaaga ttcctctctg
     3181 tttaaaactt tatccatctt tgcaaagctt tttgcaaaag cctaggcctc caaaaaagcc
     3241 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     3301 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     3361 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     3421 tctgcctgct ggggagccta agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     3481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5461 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5581 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5641 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
