LOCUS       V007642                 6457 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007642
VERSION     V007642
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6457)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6457)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      207..587
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(918..1484)
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     CDS             complement(1490..2494)
                     /gene="FAS"
                     /label="Tumor necrosis factor receptor superfamily member
                     6"
                     /note="Tumor necrosis factor receptor superfamily member 6
                     from Homo sapiens. Accession#: P25445"
     promoter        complement(2722..3900)
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             complement(3906..3975)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88466.1"
                     /translation="MDKVLNREESLQLMDLLGLERSA"
     promoter        complement(3997..4207)
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     primer_bind     complement(4236..4252)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4260..4276)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4284..4314)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4329..4350)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4638..5226)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5400..6257)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6258..6362)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
      601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
      661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
      901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
      961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
     1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
     1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
     1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
     1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
     1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
     1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
     1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
     1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag accaagcttt
     1501 ggatttcatt tctgaagttt gaattttctg agtcactagt aatgtccttg aggatgatag
     1561 tctgaatttt ctctgcaaga gtacaaagat tggctttttt gagatcttta atcaatgtgt
     1621 catacgcttc tttctttcca tgaagttgat gccaattacg aagcagttga actttctgtt
     1681 ctgctgtgtc ttggacattg tcattcttga tctcatctat tttggcttca ttgacaccat
     1741 tctttcgaac aaagccttta acttgactta gtgtcatgac tccagcaata gtggtgatat
     1801 atttactcaa gtcaacatca gataaattta ttgccactgt ttcaggattt aaggttggag
     1861 attcatgaga accttggttt tcctttctgt gctttctgca tgttttctgt acttcctttc
     1921 tcttcaccca aacaattagt ggaattggca aaagaagaag acaaagccac cccaagttag
     1981 atctggatcc ttcctctttg cacttggtgt tgctggtgag tgtgcattcc ttgatgattc
     2041 catgttcaca tttggtgcaa gggtcacagt gttcacatac agtagagtta caaaaaaagt
     2101 ttggtttaca tctgcacttg gtattctggg tccgggtgca gtttatttcc acttctaagc
     2161 catgtccttc atcacacaat ctacatcttc tgcatttgga agaaaaatgg gctttgtctg
     2221 tgtactcctt cccttcttgg cagggcacgc agtctggttc atccccattg actgtgcagt
     2281 ccctagcttt cctttcacct ggaggacagg gcttatggca gaattggcca tcatgatgca
     2341 ggccttccaa gttctgagtc tcaactgtag taacagtctt cctcaattcc aatcccttgg
     2401 agttgatgtc agtcacttgg gcattaacac ttttggacga taatctagca acagacgtaa
     2461 gaaccagagg taggagggtc cagatgccca gcatggttgt tgagcaatcc tccgaagtga
     2521 aagagcttcc ccaactccgt actgagcggg tccaccaacc cgcgggagaa gaggcagctc
     2581 cgcctgggca gccagggctg gcctcagggt gtgttccgtg ccagtgcccg cccctgaggc
     2641 tccagccaag tcactcgtaa accgcttccc tcactcccca gaagcgtcnn nctcgaggtc
     2701 gagggatctc tagagaattc ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac
     2761 tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa
     2821 ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc
     2881 catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta
     2941 atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga
     3001 cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt
     3061 cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac
     3121 tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc
     3181 aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc
     3241 tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc
     3301 ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg
     3361 ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa
     3421 agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg
     3481 ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc
     3541 ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt
     3601 aattcaaggc acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac
     3661 ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac
     3721 tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc
     3781 actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc
     3841 ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc
     3901 tcacgaggca ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt
     3961 taaaacttta tccatctttg caaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     4021 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4081 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4141 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     4201 tgcctgctgg ggagcctaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4441 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4501 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4561 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4621 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4681 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4741 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4801 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4861 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4921 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4981 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5041 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5101 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5161 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5221 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5281 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5341 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5401 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5461 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5521 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5581 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5641 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5701 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5761 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5821 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5881 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5941 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6001 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6061 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6361 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     6421 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
