LOCUS       40924_16830        5683 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pEF-BOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5683)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      207..587
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(918..1484)
                     /label=3' UTR of hGM-CSF, incl. polyA
                     /note="3' UTR of hGM-CSF, incl. polyA"
                     /regulatory_class="terminator"
     promoter        complement(1948..3126)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             complement(3132..3201)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87767.1"
                     /translation="MDKVLNREESLQLMDLLGLERSA"
     promoter        complement(3223..3433)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     complement(3462..3478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3486..3502)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3510..3540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3555..3576)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3864..4452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4626..5483)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5484..5588)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
      601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
      661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
      901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
      961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
     1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
     1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
     1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
     1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
     1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
     1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
     1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
     1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgcggcc
     1501 gcgacctgca ggcgcagaac tggtaggtat ggaagatccc tcgagatcca ttgtgctggc
     1561 ggtaggcgag cagcgcctgc ctgaagctgc gggattcccg atcagaaatg agcgccagtc
     1621 gtcgtcggct ctcggcaccg aagcgtatga ttctccgcca gcatggcttc ggccagtgcg
     1681 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg cacccccaac
     1741 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa cagtccaggg
     1801 ccggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
     1861 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgccagcac aatggatctc
     1921 gaggtcgagg gatctctaga gaattcctca cgacacctga aatggaagaa aaaaactttg
     1981 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
     2041 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
     2101 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga
     2161 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
     2221 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
     2281 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
     2341 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
     2401 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
     2461 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
     2521 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
     2581 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
     2641 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
     2701 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
     2761 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
     2821 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     2881 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
     2941 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
     3001 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
     3061 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc
     3121 ggagcctcac gaggcactcc tttcaagacc tagaaggtcc attagctgca aagattcctc
     3181 tctgtttaaa actttatcca tctttgcaaa gctttttgca aaagcctagg cctccaaaaa
     3241 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     3301 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     3361 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     3421 tacttctgcc tgctggggag cctaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     3481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5581 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     5641 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
