LOCUS       62056_8810        5235 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    519..653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(953..1541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1715..2572)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2573..2677)
                     /label=AmpR promoter
     terminator      2846..2873
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(2944..3273)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3867..4246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4247..4450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 gggcggtctg ctctaacagt gggaggccag acttaggcac agcacgatgc ccaccaccac
       61 cagtgtgccg cacaaggccg tggcggtagg gtatgtgtct gaaaatgagc tcggggagcg
      121 ggcttgcacc gctgacgcat ttggaagact taaggcagcg gcagaagaag atgcaggcag
      181 ctgagttgtt gtgttctgat aagagtcaga ggtaactccc gttgcggtgc tgttaacggt
      241 ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag
      301 ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtccttgaca
      361 cgaagcttcg cgacgtacgt tcgaacccgg gcacgtgcgg accgaattca ttgatcataa
      421 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      481 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctctacg
      661 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      721 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      781 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
      841 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
      901 tgcaggagtc gcataaggga gagcgtcgac ctcgggccgc gttgctggcg tttttccata
      961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1381 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2581 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2641 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2701 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2761 ggccctgatg gctctttgcg gcacccatcg ttcgtaatgt tccgtggcac cgaggacaac
     2821 cctcaagaga aaatgtaatc acactggctc accttcgggt gggcctttct gcgtttataa
     2881 ggagacactt tatgtttaag aaggttggta aattccttgc ggctttggca gccaagctag
     2941 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     3001 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     3061 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     3121 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     3181 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     3241 gcctggggac tttccacacc ctaactgaca cacattccac agccaagcta gcttcgtgtc
     3301 aaggacggtg actgcagtga ataataaaat gtgtgtttgt ccgaaatacg cgttttgaga
     3361 tttctgtcgc cgactaaatt catgtcgcgc gatagtggtg tttatcgccg atagagatgg
     3421 cgatattgga aaaatcggcg gccgccgata tttgaaaata tggcatattg aaaatgtcgc
     3481 cgatgtgagt ttctgtgtaa ctgatatcgc catttttcca aaagtgattt ttgggcatac
     3541 gcgatatctg gcgatagcgc ttatatcgtt tacgggggat ggcgatagac gactttggtg
     3601 acttgggcga ttctgtgtgt cgcaaatatc gcagtttcga tataggtgac agacgatatg
     3661 aggctatatc gccgatagag gcgacatcaa gctggcacat ggccaatgca tatcgatcta
     3721 tacattgaat caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     3781 tattggctat tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
     3841 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     3901 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3961 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4021 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4081 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4141 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4201 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4261 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4321 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4381 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4441 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4501 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     4561 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     4621 cttggcttct tatgcatgct atactgtttt tggcttgggg tctatacacc cccgcttcct
     4681 catgttatag gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc
     4741 actcccctat tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa
     4801 ctctctttat tggctatatg ccaatacact gtccttcaga gactgacacg gactctgtat
     4861 ttttacagga tggggtctca tttattattt acaaattcac atatacaaca ccaccgtccc
     4921 cagtgcccgc agtttttatt aaacataacg tgggatctcc acgcgaatct cgggtacgtg
     4981 ttccggacat gggctcttct ccggtagcgg cggagcttct acatccgagc cctgctccca
     5041 tgcctccagc gactcatggt cgctcggcag ctccttgctc ctaacagtgg aggccagact
     5101 taggcacagc acgatgccca ccaccaccag tgtgccgcac aaggccgtgg cggtagggta
     5161 tgtgtctgaa aatgagctcg gggagcgggc ttgcaccgct gacgcatttg gaagacttaa
     5221 ggcagcggca gaaga
//
