LOCUS       V015513                 7777 bp    DNA     circular SYN 09-MAR-2020
DEFINITION  Exported.
ACCESSION   V015513
VERSION     V015513
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (16-JAN-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
            On Mar 9, 2020 this sequence version replaced MN956534.1.
FEATURES             Location/Qualifiers
     source          1..7777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2222..3071
                     /label="Candida albicans TDH3 promoter"
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     protein_bind    3078..3102
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3103..3816
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(3820..3844)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3865..4105
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     gene            4126..4902
                     /gene="CPAR2_110290"
                     /label="CPAR2_110290"
     CDS             4126..4902
                     /codon_start=1
                     /transl_table=11
                     /gene="CPAR2_110290"
                     /product="ortholog of Candida albicans RPS1,
                     Uncharacterized in Candida parapsilosis"
                     /label="CPAR2_110290"
                     /note="recombination target"
                     /protein_id="QII68931.1"
                     /translation="MAVGKNKRLSKGKKGLKKKVVDPFTRKDWFDIKAPTTFENRNVGK
                     TLINRSTGLKNAADGLKGRVFEVCLADLQGSEDHSYRKIKLRVDEVQGKNLLTNFHGLD
                     FTSDKIRSRPLVRKWQSLVEANVTVKTADDYVLRVFAIAFTKRQPNQIKKTTYAQSSKL
                     REIRKKMTEIMQREVSNCTLAQLTSKLIPEVIGREIEKSTQTIFPLQNVHIRKVKLLKQ
                     PKFDLGSLLALHGEGSTEEKGKKVSGGFKDVVLESV"
     misc_feature    4500..4505
                     /gene="CPAR2_110290"
                     /label="StuI site for plasmid linearization"
                     /note="StuI site for plasmid linearization"
     CDS             complement(5130..6248)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      complement(6249..7123)
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     promoter        complement(7138..7156)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7163..7179)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(7320..7775)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cgtgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctcaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 gcacacacat acagacaatt gcgatatttt attttagggt caatttggaa cgtcaccata
     2281 cagtattcag tatgattaat ttgacttgca aaatacaaat cgcgtggttc aattccgaaa
     2341 aattgatagt gttccatgaa aaattacaag gagagccgtg aattataaca aatttactca
     2401 cattttggct ggcaatttct ttaaccccct taattttttt catgcagttt attattttac
     2461 gtagaagctt attctctaaa cactcggtta tccgaatata ttttgctttc agagcaattg
     2521 actgatataa taccatcaat tgaataaatc agtatcatca aatgctagtt tgagttcaat
     2581 aaaaggtata atcggtgtta actgaaaggt attgtctaat taattaggtt gcggtttttg
     2641 gatcagctgt ttcaaatcca ggctcgtggt gcacattttt tatttttgtt gtgatgattt
     2701 tttcttttct ttgcttaata gttagttagt aaaatggata atgacgcaaa attgcttctg
     2761 aatggatgat aatgccccgg gtatggcgtg tcccgtacaa atcatattat gcaaaaatag
     2821 ccaaaataga caaaaatagg tggaaattgt ttcaaccaac ccactcaacc actaaaattt
     2881 gtcttgtcaa agccaattcc aacgaaaatt ggattttttt tgacttaata tatattaacc
     2941 cttgaaattc ccttcaattg gaaaattttt ttaatttatt tatttcttct ttcttttttt
     3001 ctttttcttt ttctttcttt tattcatcac aattgaattc aaatcaatta acatcaacaa
     3061 actttacaat cgattcaaca agtttgtaca aaaaagcagg ctatgtctaa aggtgaagaa
     3121 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
     3181 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt
     3241 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttcggttat
     3301 ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt tttcaagtct
     3361 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
     3421 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
     3481 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
     3541 tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa
     3601 attagacaca acattgaaga tggttctgtt caattagctg accattatca acaaaatact
     3661 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc
     3721 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct
     3781 gctggtatta cccatggtat ggatgaattg tacaaataaa cccagctttc ttgtacaaag
     3841 tggttgctag catgaataaa tagcattata agtaaatgca tgtatactaa actcacaaat
     3901 tagagcttca atttaattat atcagttatt acccgggaat ctcggtcgta atgatttcta
     3961 taatgacgaa aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa aaaggaacta
     4021 tccaatacct cgccagaacc aagtaacagt attttacggg gcacaaatca agaacaataa
     4081 gacaggactg taaagatgga cgcatagcag atcgaattca cgcgtatggc agtaggtaaa
     4141 aataagagac tttctaaagg aaagaaagga ttgaaaaaga aggtcgtcga cccattcacc
     4201 agaaaagatt ggtttgacat taaagcccca accacctttg aaaacagaaa tgttggtaaa
     4261 actttaatca acagatccac tggtttgaag aatgcagccg atggattaaa gggaagagtt
     4321 ttcgaagttt gtttggctga cttgcaaggt tcagaagacc actcatacag aaagatcaaa
     4381 ttgagagttg atgaagttca aggtaaaaac ttgttgacca acttccacgg tttggatttc
     4441 acctcagaca aaataagatc aaggcctttg gttagaaaat ggcaatcatt ggttgaagcc
     4501 aatgttactg tcaagactgc cgatgactac gttttgagag tatttgccat tgccttcacc
     4561 aagagacaac caaaccaaat caaaaagacc acttatgctc aatcatccaa attgagagaa
     4621 atcagaaaga agatgactga aatcatgcaa agagaagttt ccaactgtac tttggctcaa
     4681 ttaacctcca agttgattcc agaagttatt ggtcgtgaaa ttgaaaaatc tactcaaact
     4741 atcttcccat tgcaaaacgt tcacatcaga aaagtcaaat tgttgaaaca accaaaattc
     4801 gacttgggtt cattgttggc tttgcacggt gaaggttcaa ctgaagaaaa gggtaagaag
     4861 gtttcaggtg gtttcaagga tgttgttttg gaatcagttt aaactagtcc ttacctaccc
     4921 attctagaaa gagccaatca gaatcaagtg aggctaccat tacttcaaat agatttattt
     4981 ccgaagatcc aagtaaaccg gtgagagaaa tttccgatat tgaattatcg caatttttag
     5041 aagaatcggt acatttttaa aaaaaagtat ttaggtggtg ttcattttgt attaataata
     5101 ttcgttaaat tttgtgtata ctttacttaa gctttttctt ttaagatttt agtaacagct
     5161 tcgacaatcg cgtcaccaac ttcagtagct gaactggtac ctcttaaatc tgcagttctg
     5221 atacctttat ccaagacttg tttcacggct tcttctaaag cttcagcttc tttcacacaa
     5281 tccaaagata atctcaacat agaagcagca gataaaatag tagcaattgg gttaacttta
     5341 ttggctggta aatcaggagc agaaccgtga catggttcat aaagaccaaa agcggtgttg
     5401 gtatctggca atgaagccaa agatgcggat ggtaataaac ccaaggaacc tggaataact
     5461 gaagcttcat cggaaatgat atcaccaaac atgttagaag tgatgattat accattcaat
     5521 ttggttgggt tttgaattaa aatcatggcg gcggagtcaa tcaattgatg ttgaacagac
     5581 aaagttggga actcttcaga aatcacttta tcgacagttc ttctccataa tctagaagaa
     5641 gctaaaacat tagccttatc caatgaccag attggtaatg gtgggttatg ttgtaaagcc
     5701 atgaaggcag ccatacgggt gattctggtg acttcatcga cggtgtattt ttcagtatcc
     5761 catgcggttt gtttatcttc actttcttct tgttcttcac gatcaccgaa ataaatacca
     5821 ccaactaatt cacgaacaat gatcaagttt gtacctttaa cgacttctgg tctcaatggg
     5881 gataattcca ataaggagtc actggcaaag ttacatggtc tgatgttagc ataaaggttc
     5941 aattctttac gaatctttaa taaaccttgt tctggacgaa cggcgccagt cccccatttt
     6001 ggtccaccaa cagcacccaa taagacggca tcagattttt tggcactttc aagtgcgtca
     6061 tctggcaatg ggacaccagt ggcatcaatg gcagcaccac cgattaaatg atgtttgaaa
     6121 tcgaaatgga ttttttggta aggagtggca gcttcaattg cttctaaaac tttgatagct
     6181 tcattgacaa tttcggtacc gacgtgatca cctggtaaaa ttgtaatagt tttagtttta
     6241 actgacattg tgggtttggg tagtgaataa aggaattaag gatgaaggaa aaaaaattct
     6301 tgagtgaaaa atttcatagc ctgagtccct cggatttttt tcgtttatta tatattttta
     6361 tagtgaacaa gaaaaaaaaa attgaacccg gctgcggaac ccacgcacgc cgttacagga
     6421 gttagtcatt cttcaacaat acgaacgtat tattcatctg gttcagatac atgaacgaaa
     6481 gtacaatttc cgaaattact ctatacacga taattacaca taacaacaca aattacaaaa
     6541 aaacaaaaaa ccttaatgtc cactccattc gatagagttc aagtcaatac cttcttgaac
     6601 catttcccat aatggagaca tctttctcaa gaaatcaact ctttcattaa ttgcttgaat
     6661 aacatagtca acttctgctt cagtggtgaa tctaccaata ccgaatctaa tggaagaatg
     6721 agccaaggca tcatcggcac ccaaagcatg taaaacatac gatggttcca aagatgcaga
     6781 ggtacatgca gaaccagaac tcaatgcaat atctttcaat gccattaaca aagattcccc
     6841 ttcaatgtat gcaaaggaaa cattcacaca acctgggtat tgatatgttg ggttattaca
     6901 accgttaaac tgagtagatg gaatggataa caaaccgttc ttcaatttag tagataacct
     6961 ctcaatatgt ttcttgtcaa atgcagattc ttgtttcatt aatcttgcag cttcaccaaa
     7021 cccagctact aatggtggtg ccaaagtacc tgatctcaaa cctctttctt gacccccacc
     7081 agtgatgatt ggatccaagg gcgaattcca gcacactggc ggcgagctcc aattcgccct
     7141 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7201 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7261 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     7321 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7381 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7441 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7501 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7561 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7621 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     7681 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     7741 acccgccgcg cttaatgcgc cgctacaggg cgcgtca
//
