LOCUS       V015878                 7764 bp    DNA     circular SYN 03-MAR-2020
DEFINITION  Exported.
ACCESSION   V015878
VERSION     V015878
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..7764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2222..3071
                     /label="Candida albicans TDH3 promoter"
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     protein_bind    3078..3102
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3103..3816
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(3820..3844)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3865..4105
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     misc_feature    4126..4499
                     /note="Candida parapsilosis NEUT5L sequence upstream;
                     recombination target"
     misc_feature    4500..4505
                     /label="StuI site for plasmid linearization"
                     /note="StuI site for plasmid linearization"
     misc_feature    4506..4889
                     /note="Candida parapsilosis NEUT5L sequence downstream;
                     recombination target"
     CDS             complement(5117..6235)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      complement(6236..7116)
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     promoter        complement(7125..7143)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7150..7166)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(7307..7762)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cgtgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctcaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 gcacacacat acagacaatt gcgatatttt attttagggt caatttggaa cgtcaccata
     2281 cagtattcag tatgattaat ttgacttgca aaatacaaat cgcgtggttc aattccgaaa
     2341 aattgatagt gttccatgaa aaattacaag gagagccgtg aattataaca aatttactca
     2401 cattttggct ggcaatttct ttaaccccct taattttttt catgcagttt attattttac
     2461 gtagaagctt attctctaaa cactcggtta tccgaatata ttttgctttc agagcaattg
     2521 actgatataa taccatcaat tgaataaatc agtatcatca aatgctagtt tgagttcaat
     2581 aaaaggtata atcggtgtta actgaaaggt attgtctaat taattaggtt gcggtttttg
     2641 gatcagctgt ttcaaatcca ggctcgtggt gcacattttt tatttttgtt gtgatgattt
     2701 tttcttttct ttgcttaata gttagttagt aaaatggata atgacgcaaa attgcttctg
     2761 aatggatgat aatgccccgg gtatggcgtg tcccgtacaa atcatattat gcaaaaatag
     2821 ccaaaataga caaaaatagg tggaaattgt ttcaaccaac ccactcaacc actaaaattt
     2881 gtcttgtcaa agccaattcc aacgaaaatt ggattttttt tgacttaata tatattaacc
     2941 cttgaaattc ccttcaattg gaaaattttt ttaatttatt tatttcttct ttcttttttt
     3001 ctttttcttt ttctttcttt tattcatcac aattgaattc aaatcaatta acatcaacaa
     3061 actttacaat cgattcaaca agtttgtaca aaaaagcagg ctatgtctaa aggtgaagaa
     3121 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
     3181 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt
     3241 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttcggttat
     3301 ggtgttcaat gttttgcgag atacccagat catatgaaac aacatgactt tttcaagtct
     3361 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
     3421 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
     3481 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
     3541 tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa
     3601 attagacaca acattgaaga tggttctgtt caattagctg accattatca acaaaatact
     3661 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc
     3721 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct
     3781 gctggtatta cccatggtat ggatgaattg tacaaataaa cccagctttc ttgtacaaag
     3841 tggttgctag catgaataaa tagcattata agtaaatgca tgtatactaa actcacaaat
     3901 tagagcttca atttaattat atcagttatt acccgggaat ctcggtcgta atgatttcta
     3961 taatgacgaa aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa aaaggaacta
     4021 tccaatacct cgccagaacc aagtaacagt attttacggg gcacaaatca agaacaataa
     4081 gacaggactg taaagatgga cgcatagcag atcgaattca cgcgtaacct catctcaagg
     4141 cgcacgtttt tcaataaatt tcaataaatt tgaaattaat taatccgatc ctccttaatt
     4201 actttgttgt ttgtttacga ttgtgataat tttagcaaaa tcaaaccaca gttttctgtt
     4261 gtttcttttt tatgttcttg acttgtagaa tagctccaag ggaaggaagg gggtgttcgg
     4321 gtgactttta tccccgtgca aagagagtag tttgtacgta gtgtacccct attgttcact
     4381 gtatcaatcc agttgccaca atttttgtaa acttcgcttt ctaaggcggg ttttctaatt
     4441 ttctttttct tgattttctt tgttgccttt tctctcgctc cacttcggtc caggtcgcta
     4501 ggcctccgac ccccactcct caccccaaaa aaaaaagaaa aaagaattac acacaaaatt
     4561 ggaatcccat tcagaaatgg aacaatcggc accaacttta caaaccattg atattcaagt
     4621 ttacgaaatg gttaaccaat gaagtgattg aatagttttc tcgtgcgatg attgtatcct
     4681 cctccatatt agccctctac ttcttatgta tatagtaagg aacagcaatt aatgaagttc
     4741 aacatgcaca ttaacacctc acctattagg cgtaatctaa aaattggaat tgattgatct
     4801 ccccatcttc ttatctctct ctctctatca caagaacggc attttttatt atcattattt
     4861 cattatgacg caatcatgta tttttgtgta ctagtcctta cctacccatt ctagaaagag
     4921 ccaatcagaa tcaagtgagg ctaccattac ttcaaataga tttatttccg aagatccaag
     4981 taaaccggtg agagaaattt ccgatattga attatcgcaa tttttagaag aatcggtaca
     5041 tttttaaaaa aaagtattta ggtggtgttc attttgtatt aataatattc gttaaatttt
     5101 gtgtatactt tacttaagct ttttctttta agattttagt aacagcttcg acaatcgcgt
     5161 caccaacttc agtagctgaa ctggtacctc ttaaatctgc agttctgata cctttatcca
     5221 agacttgttt cacggcttct tctaaagctt cagcttcttt cacacaatcc aaagataatc
     5281 tcaacataga agcagcagat aaaatagtag caattgggtt aactttattg gctggtaaat
     5341 caggagcaga accgtgacat ggttcataaa gaccaaaagc ggtgttggta tctggcaatg
     5401 aagccaaaga tgcggatggt aataaaccca aggaacctgg aataactgaa gcttcatcgg
     5461 aaatgatatc accaaacatg ttagaagtga tgattatacc attcaatttg gttgggtttt
     5521 gaattaaaat catggcggcg gagtcaatca attgatgttg aacagacaaa gttgggaact
     5581 cttcagaaat cactttatcg acagttcttc tccataatct agaagaagct aaaacattag
     5641 ccttatccaa tgaccagatt ggtaatggtg ggttatgttg taaagccatg aaggcagcca
     5701 tacgggtgat tctggtgact tcatcgacgg tgtatttttc agtatcccat gcggtttgtt
     5761 tatcttcact ttcttcttgt tcttcacgat caccgaaata aataccacca actaattcac
     5821 gaacaatgat caagtttgta cctttaacga cttctggtct caatggggat aattccaata
     5881 aggagtcact ggcaaagtta catggtctga tgttagcata aaggttcaat tctttacgaa
     5941 tctttaataa accttgttct ggacgaacgg cgccagtccc ccattttggt ccaccaacag
     6001 cacccaataa gacggcatca gattttttgg cactttcaag tgcgtcatct ggcaatggga
     6061 caccagtggc atcaatggca gcaccaccga ttaaatgatg tttgaaatcg aaatggattt
     6121 tttggtaagg agtggcagct tcaattgctt ctaaaacttt gatagcttca ttgacaattt
     6181 cggtaccgac gtgatcacct ggtaaaattg taatagtttt agttttaact gacattgtgg
     6241 gtttgggtag tgaataaagg aattaaggat gaaggaaaaa aaattcttga gtgaaaaatt
     6301 tcatagcctg agtccctcgg atttttttcg tttattatat atttttatag tgaacaagaa
     6361 aaaaaaaatt gaacccggct gcggaaccca cgcacgccgt tacaggagtt agtcattctt
     6421 caacaatacg aacgtattat tcatctggtt cagatacatg aacgaaagta caatttccga
     6481 aattactcta tacacgataa ttacacataa caacacaaat tacaaaaaaa caaaaaacct
     6541 taatgtccac tccattcgat agagttcaag tcaatacctt cttgaaccat ttcccataat
     6601 ggagacatct ttctcaagaa atcaactctt tcattaattg cttgaataac atagtcaact
     6661 tctgcttcag tggtgaatct accaataccg aatctaatgg aagaatgagc caaggcatca
     6721 tcggcaccca aagcatgtaa aacatacgat ggttccaaag atgcagaggt acatgcagaa
     6781 ccagaactca atgcaatatc tttcaatgcc attaacaaag attccccttc aatgtatgca
     6841 aaggaaacat tcacacaacc tgggtattga tatgttgggt tattacaacc gttaaactga
     6901 gtagatggaa tggataacaa accgttcttc aatttagtag ataacctctc aatatgtttc
     6961 ttgtcaaatg cagattcttg tttcattaat cttgcagctt caccaaaccc agctactaat
     7021 ggtggtgcca aagtacctga tctcaaacct ctttcttgac ccccaccagt gatgattgga
     7081 tccaagggcg aattccagca cactggcggc gagctccaat tcgccctata gtgagtcgta
     7141 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7201 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7261 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt
     7321 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     7381 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     7441 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     7501 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     7561 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     7621 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     7681 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     7741 aatgcgccgc tacagggcgc gtca
//
