LOCUS       40924_16805        4346 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Auxiliary vector pECnpt, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 4346)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4346)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2017) Agricultural Biotechnology Research Center, Academia 
            Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 11529, 
            Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..227
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     primer_bind     complement(258..274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(282..298)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(306..336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(351..372)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(660..1248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1422..2279)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2280..2384)
                     /label=AmpR promoter
     primer_bind     2858..2874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2887..3134
                     /note="atpI promoter; PatpI"
                     /regulatory_class="promoter"
     misc_feature    3135..3150
                     /label=cloning site
                     /note="cloning site"
     regulatory      3151..3319
                     /note="rps16 terminator; Trps16"
                     /regulatory_class="terminator"
     regulatory      3326..3544
                     /note="rrn promoter; Prrn"
                     /regulatory_class="promoter"
     CDS             3551..4342
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctagagatcc tggcctagtc tataggaggt tttgaaaaga aaggagcaat aatcattttc
       61 ttgttctatc aagagggtgc tattgctcct ttcttttttt ctttttattt atttactagt
      121 attttactta catagacttt tttgtttaca ttatagaaaa agaaggagag gttattttct
      181 tgcatttatt catgattgag tattctattt tgattttgta tttgtttctg caggcatgca
      241 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      301 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      361 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      421 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2281 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2341 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2401 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2461 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     2521 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     2581 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     2641 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     2701 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     2761 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     2821 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     2881 gagctctcta gctatataag aaatccttga ttaataataa cataataaga taaataactt
     2941 acttcagaaa tcccttatgg aatcgcttac tatttctgaa tttcaaaaaa gagataaaaa
     3001 tagctgggga tattatgtga tttattagta ttctaaatct tagttggtat tcaaaatatc
     3061 cgattcaagt agacaaagag atggttgaat caaaaaattt tgtttaaagt tcaatttttt
     3121 cagagggcaa ggcagcggcc gcggcgcgcc tcaaccgaaa ttcaattaag gaaataaatt
     3181 aaggaaatac aaaaaggggg gtagtcattt gtatataact ttgtatgact tttctcttct
     3241 atttttttgt atttcctccc tttccttttc tatttgtatt tttttatcat tgcttccatt
     3301 gaattccgtg ttctataacg gtaccatcag ttcgagcctg attatcccta agcccaatgt
     3361 gagtttttct agttggattt gctcccccgc cgtcgttcaa tgagaatgga taagaggctc
     3421 gtgggattga cgtgaggggg cagggatggc tatatttctg ggagcgaact ccgggcgaat
     3481 atgaagcgca tggatacaag ttatgccttg gaatgaaaga caattccgaa tccgctttgt
     3541 ctacggatcc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3601 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3661 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3721 gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3781 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3841 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     3901 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     3961 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4021 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4081 gatgcccgac ggcgaggatc tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat
     4141 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4201 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4261 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4321 tcgccttctt gacgagttct tctgat
//
