LOCUS       40924_16810        9525 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pECSBAC4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   SanMiguel PJ, Lin J-Y., Jackson SA.
  TITLE     The complete sequence of the bacterial artificial chromosome vector,
            pECSBAC4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   SanMiguel PJ, Lin J-Y., Jackson S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2006) Department of Horticulture and Landscape 
            Architecture, Purdue University, 170 South University Street, West 
            Lafayette, IN 47907-2072, USA
REFERENCE   3  (bases 1 to 9525)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-SEP-2006) Department of Horticulture and Landscape Architecture,
            Purdue University, 170 South University Street, West Lafayette, IN 
            47907-2072, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        218..236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    239..295
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(302..320)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(338..354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(362..378)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(386..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(431..452)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(925..1713)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2693..3349)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3350..3452)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      4379..4598
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4689..5441
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5447..5697
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6023..7195
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7198..8166
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    8242..8715
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(8975..9373)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9391..9424)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
      241 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttgagta
      301 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgaa ccccttgcgg
      541 ccgcccagct ttatgcttgt aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga
      601 cctctagggt ccccaattaa ttagtaatat aatctattaa aggtcattca aaaggtcatc
      661 caccggatca gcttagtaaa gccctcgcta gattttaatg cggatgttgc gattacttcg
      721 ccaactattg cgataacaag aaaaagccag cctttcatga tatatctccc aatttgtgta
      781 gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg
      841 agcaattatg tgcttagtgc atctaacgct tgagttaagc cgcgccgcga agcggcgtcg
      901 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
      961 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
     1021 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
     1081 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
     1141 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
     1201 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
     1261 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
     1321 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
     1381 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
     1441 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
     1501 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
     1561 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
     1621 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
     1681 agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt tagggcgact
     1741 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     1801 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
     1861 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1921 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1981 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     2041 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     2101 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     2161 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     2221 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccagcttct
     2281 gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg atctggattt
     2341 cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg ccttgatgtt
     2401 acccgagagc ttggcaccca gcctgcgcga gcaggggaat tgatccggtg gatgaccttt
     2461 tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt
     2521 tattttaaaa attttttcac aaaacggttt acaagcataa agctgggccg tcgaccaatt
     2581 ctcatgtttg acagcttatc atcgaatttc tgccattcat ccgcttatta tcacttattc
     2641 aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     2701 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2761 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2821 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2881 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     2941 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     3001 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     3061 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     3121 ttgccatacg taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     3181 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     3241 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     3301 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     3361 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     3421 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     3481 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     3541 tcacaggtat ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca
     3601 gagaaagcgc ggatctggga agtgacggac agaacggtca ggacctggat tggggaggcg
     3661 gttgccgccg ctgctgctga cggtgtgacg ttctctgttc cggtcacacc acatacgttc
     3721 cgccattcct atgcgatgca catgctgtat gccggtatac cgctgaaagt tctgcaaagc
     3781 ctgatgggac ataagtccat cagttcaacg gaagtctaca cgaaggtttt tgcgctggat
     3841 gtggctgccc ggcaccgggt gcagtttgcg atgccggagt ctgatgcggt tgcgatgctg
     3901 aaacaattat cctgagaata aatgccttgg cctttatatg gaaatgtgga actgagtgga
     3961 tatgctgttt ttgtctgtta aacagagaag ctggctgtta tccactgaga agcgaacgaa
     4021 acagtcggga aaatctccca ttatcgtaga gatccgcatt attaatctca ggagcctgtg
     4081 tagcgtttat aggaagtagt gttctgtcat gatgcctgca agcggtaacg aaaacgattt
     4141 gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt tacgttgaag tggagcggat
     4201 tatgtcagca atggacagaa caacctaatg aacacagaac catgatgtgg tctgtccttt
     4261 tacagccagt agtgctcgcc gcagtcgagc gacagggcga agccctcgag tgagcgagga
     4321 agcaccaggg aacagcactt atatattctg cttacacacg atgcctgaaa aaacttccct
     4381 tggggttatc cacttatcca cggggatatt tttataatta ttttttttat agtttttaga
     4441 tcttcttttt tagagcgcct tgtaggcctt tatccatgct ggttctagag aaggtgttgt
     4501 gacaaattgc cctttcagtg tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa
     4561 ttgcccttaa ccctgtgaca aattgccctc agaagaagct gttttttcac aaagttatcc
     4621 ctgcttattg actctttttt atttagtgtg acaatctaaa aacttgtcac acttcacatg
     4681 gatctgtcat ggcggaaaca gcggttatca atcacaagaa acgtaaaaat agcccgcgaa
     4741 tcgtccagtc aaacgacctc actgaggcgg catatagtct ctcccgggat caaaaacgta
     4801 tgctgtatct gttcgttgac cagatcagaa aatctgatgg caccctacag gaacatgacg
     4861 gtatctgcga gatccatgtt gctaaatatg ctgaaatatt cggattgacc tctgcggaag
     4921 ccagtaagga tatacggcag gcattgaaga gtttcgcggg gaaggaagtg gttttttatc
     4981 gccctgaaga ggatgccggc gatgaaaaag gctatgaatc ttttccttgg tttatcaaac
     5041 gtgcgcacag tccatccaga gggctttaca gtgtacatat caacccatat ctcattccct
     5101 tctttatcgg gttacagaac cggtttacgc agtttcggct tagtgaaaca aaagaaatca
     5161 ccaatccgta tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag ccggatggct
     5221 caggcatcgt ctctctgaaa atcgactgga tcatagagcg ttaccagctg cctcaaagtt
     5281 accagcgtat gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca
     5341 gcagaactcc aatgcgcctc tcatacattg agaaaaagaa aggccgccag acgactcata
     5401 tcgtattttc cttccgcgat atcacttcca tgacgacagg atagtctgag ggttatctgt
     5461 cacagatttg agggtggttc gtcacatttg ttctgaccta ctgagggtaa tttgtcacag
     5521 ttttgctgtt tccttcagcc tgcatggatt ttctcatact ttttgaactg taatttttaa
     5581 ggaagccaaa tttgagggca gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg
     5641 tgacctgata tcgggggtta gttcgtcatc attgatgagg gttgattatc acagtttatt
     5701 actctgaatt ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt
     5761 tcttcttgcg ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca
     5821 gttcgctcgc tatgctcggt tacacggctg cggcgagcgc tagtgataat aagtgactga
     5881 ggtatgtgct cttcttatct ccttttgtag tgttgctctt attttaaaca actttgcggt
     5941 tttttgatga ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa
     6001 aacgcaaagc aatgattaaa ggatgttcag aatgaaactc atggaaacac ttaaccagtg
     6061 cataaacgct ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta atgatgacag
     6121 cccggaagcg aggaaaataa cccggcgctg gagaataggt gaagcagcgg atttagttgg
     6181 ggtttcttct caggctatca gagatgccga gaaagcaggg cgactaccgc acccggatat
     6241 ggaaattcga ggacgggttg agcaacgtgt tggttataca attgaacaaa ttaatcatat
     6301 gcgtgatgtg tttggtacgc gattgcgacg tgctgaagac gtatttccac cggtgatcgg
     6361 ggttgctgcc cataaaggtg gcgtttacaa aacctcagtt tctgttcatc ttgctcagga
     6421 tctggctctg aaggggctac gtgttttgct cgtggaaggt aacgaccccc agggaacagc
     6481 ctcaatgtat cacggatggg taccagatct tcatattcat gcagaagaca ctctcctgcc
     6541 tttctatctt ggggaaaagg acgatgtcac ttatgcaata aagcccactt gctggccggg
     6601 gcttgacatt attccttcct gtctggctct gcaccgtatt gaaactgagt taatgggcaa
     6661 atttgatgaa ggtaaactgc ccaccgatcc acacctgatg ctccgactgg ccattgaaac
     6721 tgttgctcat gactatgatg tcatagttat tgacagcgcg cctaacctgg gtatcggcac
     6781 gattaatgtc gtatgtgctg ctgatgtgct gattgttccc acgcctgctg agttgtttga
     6841 ctacacctcc gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga acgttgatct
     6901 taaagggttc gagcctgatg tacgtatttt gcttaccaaa tacagcaata gtaatggctc
     6961 tcagtccccg tggatggagg agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa
     7021 tgttgtacgt gaaacggatg aagttggtaa aggtcagatc cggatgagaa ctgtttttga
     7081 acaggccatt gatcaacgct cttcaactgg tgcctggaga aatgctcttt ctatttggga
     7141 acctgtctgc aatgaaattt tcgatcgtct gattaaacca cgctgggaga ttagataatg
     7201 aagcgtgcgc ctgttattcc aaaacatacg ctcaatactc aaccggttga agatacttcg
     7261 ttatcgacac cagctgcccc gatggtggat tcgttaattg cgcgcgtagg agtaatggct
     7321 cgcggtaatg ccattacttt gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg
     7381 ctccggggtg atagtgttga gaagacctct cgggtatggt caggtaatga acgtgaccag
     7441 gagctgctta ctgaggacgc actggatgat ctcatccctt cttttctact gactggtcaa
     7501 cagacaccgg cgttcggtcg aagagtatct ggtgtcatag aaattgccga tgggagtcgc
     7561 cgtcgtaaag ctgctgcact taccgaaagt gattatcgtg ttctggttgg cgagctggat
     7621 gatgagcaga tggctgcatt atccagattg ggtaacgatt atcgcccaac aagtgcttat
     7681 gaacgtggtc agcgttatgc aagccgattg cagaatgaat ttgctggaaa tatttctgcg
     7741 ctggctgatg cggaaaatat ttcacgtaag attattaccc gctgtatcaa caccgccaaa
     7801 ttgcctaaat cagttgttgc tcttttttct caccccggtg aactatctgc ccggtcaggt
     7861 gatgcacttc aaaaagcctt tacagataaa gaggaattac ttaagcagca ggcatctaac
     7921 cttcatgagc agaaaaaagc tggggtgata tttgaagctg aagaagttat cactctttta
     7981 acttctgtgc ttaaaacgtc atctgcatca agaactagtt taagctcacg acatcagttt
     8041 gctcctggag cgacagtatt gtataagggc gataaaatgg tgcttaacct ggacaggtct
     8101 cgtgttccaa ctgagtgtat agagaaaatt gaggccattc ttaaggaact tgaaaagcca
     8161 gcaccctgat gcgaccacgt tttagtctac gtttatctgt ctttacttaa tgtcctttgt
     8221 tacaggccag aaagcataac tggcctgaat attctctctg ggcccactgt tccacttgta
     8281 tcgtcggtct gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     8341 agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc
     8401 ccactcgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt
     8461 ctgattatta gtctgggacc atggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     8521 accacggtcc cactcgtatc gtcggtctga ttattagtct ggaaccacgg tcccactcgt
     8581 atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat
     8641 tagtctggga ccacgatccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt
     8701 cccacttgta ttgtcgatca gactatcagc gtgagactac gattccatca atgcctgtca
     8761 agggcaagta ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg
     8821 tatgcctgct gtggattgct gctgtgtcct gcttatccac aacattttgc gcacggttat
     8881 gtggacaaaa tacctggtta cccaggccgt gccggcacgt taaccgggct gcatccgatg
     8941 caagtgtgtc gctgtcgacg agctcgcgag ctcggacatg aggttgcccc gtattcagtg
     9001 tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac
     9061 gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc acgttgtgat
     9121 atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac aggttacggg
     9181 gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt
     9241 cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac
     9301 aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga
     9361 atgaacaatg gaagtccgag ctcatcgcta ataacttcgt atagcataca ttatacgaag
     9421 ttatattcga tgcggccgca aggggttcgc gtcagcgggt gttggcgggt gtcggggctg
     9481 gcttaactat gcggcatcag agcagattgt actgagagtg cacca
//
