LOCUS       40924_16680        6213 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pEC-T19mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 6213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             400..1863
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO66613.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            400..1863
                     /gene="repA"
                     /label=repA
     CDS             2506..2721
                     /codon_start=1
                     /gene="per"
                     /product="Per"
                     /label=per
                     /note="positive effector of replication"
                     /protein_id="AAO66614.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2506..2721
                     /gene="per"
                     /label=per
     CDS             complement(3677..3688)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     oriT            complement(4892..4999)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5211..5799
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6087..6108
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6123..6153
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6161..6177
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6185..6201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat aagctagatc cccatcaatc
      241 ctgcctattt gccacgttta acaaggtagt taagcgttca tttacgaaga aaacacgata
      301 agctgcacaa atacctgaaa aagttgaacg ccccgtgagc gggaactcac agggcgtcgg
      361 ctaaccccca gtcatcagct gggagaaagc actcaagaca tgactctagc cgatccgcag
      421 gacacagtca cagctagcgc gtggaaattg tccgccgatc tgttcgacac ccaccccgaa
      481 gctatgcgct gcggctcacg cggctggacg gcagaagatc gccgcgaact gctcgctcac
      541 ctgggacgcg aaagcttcca gggcagcaag acaagagatt tcgcgagcgc ctggattaaa
      601 aacccggata ccggcgaaac ccaaccaaag ctctaccggg ctggctcaaa agcgctgacg
      661 cggtgccagt acgttgcgct gacgcacgcg caacatgccg cggtgatcgt gcttgacatc
      721 gatgtgccca gccaccaggc cggcgggaag attgagcacg taaacccgca ggtctacgcg
      781 attttagaga aatgggcacg cctagaaaaa gcgccggctt ggatcggcgt gaatccgctg
      841 agcgggaaat gccagctcat ctggctcatt gacccggtgt atgccgcagc aggtaaaacc
      901 agcccaaata tgcgcctgct ggctgcaacg acggaagaaa tgactcgtgt tttcggcgct
      961 gaccaggctt tttcgcatag gctgagccgg tggccgctgc acgtctcaga cgatccgaca
     1021 gcctataaat ggcactgcca gcatgatcgt gtggatcggc tggccgacct aatggagatt
     1081 gctcgaacga tgaccggatc acagaagccg aaaaagtaca ttgagcagga cttttccagc
     1141 ggacgcgccc gcattgaagc ggcacaacgc gccaccgcag aagccaaggc gctagcgatt
     1201 ttggacgcga gcctgccgag cgccctggac gcgtccggcg acctgatcga cggcgtgcga
     1261 gtgctctgga caaatccaga gcgagcgcgc gacgagaccg cgtttcgcca cgcgttgacc
     1321 gtgggatacc agctcaaagc tgctggtgag cgcctaaaag atgccaagat catcgacgcg
     1381 tatgaagtgg cgtacaacgt tgcccaggcg gtcggtgcag acggccggga gccggatctt
     1441 cccgccatgc gtgatcgcct gacgatggcg cgtcgtgtgc gcggctacgt ggctaaaggc
     1501 cagccagtcg tccctgctcg tcgggtggaa acgcagagca gccgagggcg gaaagctcta
     1561 gcgacgatgg ggcgacgggg cgcagctaca tcgaatgcac gcagatgggc tgacccagaa
     1621 agtaagtatg cgcaggagac gcgacagcga ttagcggaag caaacaaacg ccgagaaatg
     1681 acaggcgagt tgctcgaact tcgcgtcaaa actgcgatcc tggatgcccg ttctcaatcg
     1741 gttgctgatc cctcgactcg tgagcttgca ggcgaactag gtgtcagtga aaggcgcatc
     1801 caacaagtca gaaaggcact tggaatggaa gctaaacgcg gccgtccacg ggctgaaaac
     1861 taataaacga aacaccgtca gcagaaaacg gttcccccct ttaggggtcc cgtccttgct
     1921 ctggctctca cttgccctca ccctccgcta tccacgggct gaaaactaat aaacgaaaca
     1981 ccgtcagcag aaaacggttc ccccccttta gggtgtctcg ctcctagctc tgatccctcc
     2041 ccggttcctc cccggcctga tttttaaggg gggctcacgc tgtcggcaga gaacggttcc
     2101 ccgccttctg ctctggctct tcctcgactc cctccccctc aaaaatctcc tcgagatcct
     2161 ggagaccttt ttggagctag cgcgttgctg cttcgcacca acttgctcat gatgattttc
     2221 atttttgctt gtgtgctttt ttgggttgaa ccctccaaag aggggaaacc aggggcacac
     2281 ctcatgcact aaagtgccgc ttcgctggtc agggtgaaat cacctggaaa aaaagtgcgg
     2341 taaccgctgc gcttggcgtt ttttctgggc aagaagtctc gcaggttttc gcaggagtgc
     2401 cggaagaaat tatcagaatt ggggctagaa tttttaacga acgttcgtta taatggtgtc
     2461 atgaccttca cgacgaagta ccaaaactgg cctgaagcat cagcggtgga tctctccgat
     2521 gtcgcgctgg agtccgacgc actcgatgcc gccgtcgatt taaaaacggt gatcggattt
     2581 ttccgcgccc tcgatacgac agacgcgcca gcatcacgcg actgggcaag tgccgcgagc
     2641 gacctagaaa cgcttgtggc cgaccttgaa gagctggccg acgagctgcg tgctcggcag
     2701 cgccaggagg acgcgcagta gtggaggatc gcatcagctg cgcctactgc ggtggcctga
     2761 tcccaccccg gcctgaccca cgaggacggc gcgcaaaata ctgctcagac gcgtgtcgtg
     2821 ccgcagccag ccgcgagcgc gccaacaagc gccacgccca ggaggtcgaa gccgcacgtc
     2881 atctagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     2941 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     3001 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     3061 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     3121 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     3181 ggatctgatg gcgcagggga tcaagatcct ccttcgtcgg tgtcgtcgcc ggatggtctg
     3241 cggtggtgct cagcgtggag acgcgcaccg tcacggaccg cgtgtagtgc gtggcggaaa
     3301 cttcttgcgt ttcgcaagag aaatgcgtcc catttctcgt cggactcggg gaaggaagcg
     3361 tgatgctctc ggtcaagcac gtcgctcgcc agcgctgcga ggagttcggc cttcgtgcgg
     3421 aagtgccagt agaggccggg ctgctgtacc tgtaagtgag ccgccagcgc gcgagtggtg
     3481 aagccatcga gcccagtctc gtcgagcacc tgccgggccc cgagcaacac ggacgtgcgg
     3541 tcgagacgct tccggtggtg agtcatagtt gcactttatc atcgataact ttatcttaga
     3601 taaagtgact gctcgctact ctcatctgac tgctcgctac tctcatcgtg gaatcctgac
     3661 agccgtgctc atcacggcga ccctcgatgc tgcagggctg ggcctcgtga tgccgatctt
     3721 gcctaccctt ctcgaccagg tcggtgcccc cgacgacatg atcccactgc acgtcggact
     3781 actgacagcg ctctatgcga tcatgcagtt tctttgcgcc ccgatccttg gccgactctc
     3841 tgaccgtttc ggacgccgcc gcgtgcttgt cgcctccctc gcaggcgcga cgatcgacta
     3901 cctcgtgctc gcactgacgg acacgctgtg ggtcttttac ctcgcccgcg cggttgcagg
     3961 cattaccggc gccacgaacg ccgtcaccgc gacggtgatc gccgacatta ctccgccgga
     4021 tcagcgcgca aaacgctacg ggtggctcgg cgcatgctac ggcggtggca tgatcgcggg
     4081 tcccgccatt ggcggtcttt tcggcggggt ctcaccgcat ctgccattcc tcgtcgccgc
     4141 cgcgctcgcc ggaatcaccc tcgtactcag cgcgagtctt ctgcgtgaga cgcggccacc
     4201 gggcagcaac ggctcgcacg cacagcaacc cggtacggcg aagcgaaccg cagtgccggg
     4261 gatgcttatc cttctcgcag tcttcggcat cgtgcagttc atcggccaag caccaggctc
     4321 cacctgggtg ctcttcacgc agcagcgcct cgactggaac cccgtcgaag tcggcgtttc
     4381 gctatccatc ttcggaatgg tgcaagtatt cgtgcaggcg gcactgaccg gacgcatcgt
     4441 gtcccggatc ggcgagaccc gggcgatcct cgtcggtatc gccgcagacg ccattgggct
     4501 catcggcctt gccctcatcg ccagcacatg ggcgatgcta ccgatcctcg cagcgctcgg
     4561 actcggcagc atcacgttgc ccgcactgca gacgctgctc tcgagacgcg cgcccgagca
     4621 gcagcaggga cgcctgcagg gaacacttgc aagcctgaac agcctcacct cgatcatcgg
     4681 cccggtcacc ttcaccggca ttttcgcact cacccgaacg aatgcagacg gcaccctctg
     4741 gatctgcgcc gcagcgctct acgttctctg cgccctcctg atgatccgtg agacatgcgc
     4801 ctcacggcga tctcgataac cgcgctaagg tgccatcccg atgcgacggg atcgctctgc
     4861 caccagtcaa gtctcccgta gccggtatga gggccagcct cgcagagcag gattcccgtt
     4921 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     4981 tacttcacct atcctgcccc gctgacgccg ttggatacac caaggaaagt ctacacgaac
     5041 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     5101 aatgaccccg aagcagggtt atgcagcgga aaagccatga ccaaaatccc ttaacgtgag
     5161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5341 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5581 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6181 cacacaggaa acagctatga ccatgattac gcc
//
