LOCUS       40924_16675        6209 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEC-T18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Efficient electrotransformation of corynebacterium diphtheriae with 
            a mini-replicon derived from the Corynebacterium glutamicum plasmid 
            pGA1
  JOURNAL   Curr. Microbiol. 45 (5), 362-367 (2002)
  PUBMED    12232668
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2001) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 6209)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2002"; volume: "45"; issue: "5"; pages: 
            "362-367"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2001) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             184..1647
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /note="Rep"
                     /protein_id="AAL38583.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            184..1647
                     /gene="rep"
                     /label=rep
     CDS             2290..2505
                     /codon_start=1
                     /gene="per"
                     /product="positive effector of replication"
                     /label=per
                     /note="Per"
                     /protein_id="AAL38584.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2290..2505
                     /gene="per"
                     /label=per
     CDS             complement(3457..3468)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     oriT            complement(4672..4779)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4991..5579
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5867..5888
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5903..5933
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5941..5957
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5965..5981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5991..6047
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6051..6067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgataagcta gatccccatc aatcctgcct atttgccacg tttaacaagg tagttaagcg
       61 ttcatttacg aagaaaacac gataagctgc acaaatacct gaaaaagttg aacgccccgt
      121 gagcgggaac tcacagggcg tcggctaacc cccagtcatc agctgggaga aagcactcaa
      181 gacatgactc tagccgatcc gcaggacaca gtcacagcta gcgcgtggaa attgtccgcc
      241 gatctgttcg acacccaccc cgaagctatg cgctgcggct cacgcggctg gacggcagaa
      301 gatcgccgcg aactgctcgc tcacctggga cgcgaaagct tccagggcag caagacaaga
      361 gatttcgcga gcgcctggat taaaaacccg gataccggcg aaacccaacc aaagctctac
      421 cgggctggct caaaagcgct gacgcggtgc cagtacgttg cgctgacgca cgcgcaacat
      481 gccgcggtga tcgtgcttga catcgatgtg cccagccacc aggccggcgg gaagattgag
      541 cacgtaaacc cgcaggtcta cgcgatttta gagaaatggg cacgcctaga aaaagcgccg
      601 gcttggatcg gcgtgaatcc gctgagcggg aaatgccagc tcatctggct cattgacccg
      661 gtgtatgccg cagcaggtaa aaccagccca aatatgcgcc tgctggctgc aacgacggaa
      721 gaaatgactc gtgttttcgg cgctgaccag gctttttcgc ataggctgag ccggtggccg
      781 ctgcacgtct cagacgatcc gacagcctat aaatggcact gccagcatga tcgtgtggat
      841 cggctggccg acctaatgga gattgctcga acgatgaccg gatcacagaa gccgaaaaag
      901 tacattgagc aggacttttc cagcggacgc gcccgcattg aagcggcaca acgcgccacc
      961 gcagaagcca aggcgctagc gattttggac gcgagcctgc cgagcgccct ggacgcgtcc
     1021 ggcgacctga tcgacggcgt gcgagtgctc tggacaaatc cagagcgagc gcgcgacgag
     1081 accgcgtttc gccacgcgtt gaccgtggga taccagctca aagctgctgg tgagcgccta
     1141 aaagatgcca agatcatcga cgcgtatgaa gtggcgtaca acgttgccca ggcggtcggt
     1201 gcagacggcc gggagccgga tcttcccgcc atgcgtgatc gcctgacgat ggcgcgtcgt
     1261 gtgcgcggct acgtggctaa aggccagcca gtcgtccctg ctcgtcgggt ggaaacgcag
     1321 agcagccgag ggcggaaagc tctagcgacg atggggcgac ggggcgcagc tacatcgaat
     1381 gcacgcagat gggctgaccc agaaagtaag tatgcgcagg agacgcgaca gcgattagcg
     1441 gaagcaaaca aacgccgaga aatgacaggc gagttgctcg aacttcgcgt caaaactgcg
     1501 atcctggatg cccgttctca atcggttgct gatccctcga ctcgtgagct tgcaggcgaa
     1561 ctaggtgtca gtgaaaggcg catccaacaa gtcagaaagg cacttggaat ggaagctaaa
     1621 cgcggccgtc cacgggctga aaactaataa acgaaacacc gtcagcagaa aacggttccc
     1681 ccctttaggg gtcccgtcct tgctctggct ctcacttgcc ctcaccctcc gctatccacg
     1741 ggctgaaaac taataaacga aacaccgtca gcagaaaacg gttccccccc tttagggtgt
     1801 ctcgctccta gctctgatcc ctccccggtt cctccccggc ctgattttta aggggggctc
     1861 acgctgtcgg cagagaacgg ttccccgcct tctgctctgg ctcttcctcg actccctccc
     1921 cctcaaaaat ctcctcgaga tcctggagac ctttttggag ctagcgcgtt gctgcttcgc
     1981 accaacttgc tcatgatgat tttcattttt gcttgtgtgc ttttttgggt tgaaccctcc
     2041 aaagagggga aaccaggggc acacctcatg cactaaagtg ccgcttcgct ggtcagggtg
     2101 aaatcacctg gaaaaaaagt gcggtaaccg ctgcgcttgg cgttttttct gggcaagaag
     2161 tctcgcaggt tttcgcagga gtgccggaag aaattatcag aattggggct agaattttta
     2221 acgaacgttc gttataatgg tgtcatgacc ttcacgacga agtaccaaaa ctggcctgaa
     2281 gcatcagcgg tggatctctc cgatgtcgcg ctggagtccg acgcactcga tgccgccgtc
     2341 gatttaaaaa cggtgatcgg atttttccgc gccctcgata cgacagacgc gccagcatca
     2401 cgcgactggg caagtgccgc gagcgaccta gaaacgcttg tggccgacct tgaagagctg
     2461 gccgacgagc tgcgtgctcg gcagcgccag gaggacgcgc agtagtggag gatcgcatca
     2521 gctgcgccta ctgcggtggc ctgatcccac cccggcctga cccacgagga cggcgcgcaa
     2581 aatactgctc agacgcgtgt cgtgccgcag ccagccgcga gcgcgccaac aagcgccacg
     2641 cccaggaggt cgaagccgca cgtcatctag cttcacgctg ccgcaagcac tcagggcgca
     2701 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     2761 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     2821 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     2881 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     2941 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaact ccttcgtcgg
     3001 tgtcgtcgcc ggatggtctg cggtggtgct cagcgtggag acgcgcaccg tcacggaccg
     3061 cgtgtagtgc gtggcggaaa cttcttgcgt ttcgcaagag aaatgcgtcc catttctcgt
     3121 cggactcggg gaaggaagcg tgatgctctc ggtcaagcac gtcgctcgcc agcgctgcga
     3181 ggagttcggc cttcgtgcgg aagtgccagt agaggccggg ctgctgtacc tgtaagtgag
     3241 ccgccagcgc gcgagtggtg aagccatcga gcccagtctc gtcgagcacc tgccgggccc
     3301 cgagcaacac ggacgtgcgg tcgagacgct tccggtggtg agtcatagtt gcactttatc
     3361 atcgataact ttatcttaga taaagtgact gctcgctact ctcatctgac tgctcgctac
     3421 tctcatcgtg gaatcctgac agccgtgctc atcacggcga ccctcgatgc tgcagggctg
     3481 ggcctcgtga tgccgatctt gcctaccctt ctcgaccagg tcggtgcccc cgacgacatg
     3541 atcccactgc acgtcggact actgacagcg ctctatgcga tcatgcagtt tctttgcgcc
     3601 ccgatccttg gccgactctc tgaccgtttc ggacgccgcc gcgtgcttgt cgcctccctc
     3661 gcaggcgcga cgatcgacta cctcgtgctc gcactgacgg acacgctgtg ggtcttttac
     3721 ctcgcccgcg cggttgcagg cattaccggc gccacgaacg ccgtcaccgc gacggtgatc
     3781 gccgacatta ctccgccgga tcagcgcgca aaacgctacg ggtggctcgg cgcatgctac
     3841 ggcggtggca tgatcgcggg tcccgccatt ggcggtcttt tcggcggggt ctcaccgcat
     3901 ctgccattcc tcgtcgccgc cgcgctcgcc ggaatcaccc tcgtactcag cgcgagtctt
     3961 ctgcgtgaga cgcggccacc gggcagcaac ggctcgcacg cacagcaacc cggtacggcg
     4021 aagcgaaccg cagtgccggg gatgcttatc cttctcgcag tcttcggcat cgtgcagttc
     4081 atcggccaag caccaggctc cacctgggtg ctcttcacgc agcagcgcct cgactggaac
     4141 cccgtcgaag tcggcgtttc gctatccatc ttcggaatgg tgcaagtatt cgtgcaggcg
     4201 gcactgaccg gacgcatcgt gtcccggatc ggcgagaccc gggcgatcct cgtcggtatc
     4261 gccgcagacg ccattgggct catcggcctt gccctcatcg ccagcacatg ggcgatgcta
     4321 ccgatcctcg cagcgctcgg actcggcagc atcacgttgc ccgcactgca gacgctgctc
     4381 tcgagacgcg cgcccgagca gcagcaggga cgcctgcagg gaacacttgc aagcctgaac
     4441 agcctcacct cgatcatcgg cccggtcacc ttcaccggca ttttcgcact cacccgaacg
     4501 aatgcagacg gcaccctctg gatctgcgcc gcagcgctct acgttctctg cgccctcctg
     4561 atgatccgtg agacatgcgc ctcacggcga tctcgataac cgcgctaagg tgccatcccg
     4621 atgcgacggg atcgctctgc caccagtcaa gtctcccgta gccggtatga gggccagcct
     4681 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     4741 cacccgctcg cgggtgggcc tacttcacct atcctgcccc gctgacgccg ttggatacac
     4801 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     4861 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagccatga
     4921 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4981 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5041 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5101 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5161 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5221 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5281 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5341 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     5401 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5461 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5521 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5581 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5641 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5701 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5761 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5821 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5881 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5941 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc
     6001 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc
     6061 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6181 cagttgcgca gcctgaatgg cgaatggcg
//
