LOCUS       40924_16670        5711 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pEC-S19mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 5711)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             400..1863
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO66610.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            400..1863
                     /gene="repA"
                     /label=repA
     CDS             2506..2721
                     /codon_start=1
                     /gene="per"
                     /product="Per"
                     /label=per
                     /note="positive effector of replication"
                     /protein_id="AAO66611.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2506..2721
                     /gene="per"
                     /label=per
     CDS             3480..4247
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin adenylyltransferase"
                     /protein_id="AAO66612.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            3480..4247
                     /gene="aad9"
                     /label=aad9
     oriT            complement(4389..4497)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4709..5297
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5585..5606
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5621..5651
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5659..5675
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5683..5699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat aagctagatc cccatcaatc
      241 ctgcctattt gccacgttta acaaggtagt taagcgttca tttacgaaga aaacacgata
      301 agctgcacaa atacctgaaa aagttgaacg ccccgtgagc gggaactcac agggcgtcgg
      361 ctaaccccca gtcatcagct gggagaaagc actcaagaca tgactctagc cgatccgcag
      421 gacacagtca cagctagcgc gtggaaattg tccgccgatc tgttcgacac ccaccccgaa
      481 gctatgcgct gcggctcacg cggctggacg gcagaagatc gccgcgaact gctcgctcac
      541 ctgggacgcg aaagcttcca gggcagcaag acaagagatt tcgcgagcgc ctggattaaa
      601 aacccggata ccggcgaaac ccaaccaaag ctctaccggg ctggctcaaa agcgctgacg
      661 cggtgccagt acgttgcgct gacgcacgcg caacatgccg cggtgatcgt gcttgacatc
      721 gatgtgccca gccaccaggc cggcgggaag attgagcacg taaacccgca ggtctacgcg
      781 attttagaga aatgggcacg cctagaaaaa gcgccggctt ggatcggcgt gaatccgctg
      841 agcgggaaat gccagctcat ctggctcatt gacccggtgt atgccgcagc aggtaaaacc
      901 agcccaaata tgcgcctgct ggctgcaacg acggaagaaa tgactcgtgt tttcggcgct
      961 gaccaggctt tttcgcatag gctgagccgg tggccgctgc acgtctcaga cgatccgaca
     1021 gcctataaat ggcactgcca gcatgatcgt gtggatcggc tggccgacct aatggagatt
     1081 gctcgaacga tgaccggatc acagaagccg aaaaagtaca ttgagcagga cttttccagc
     1141 ggacgcgccc gcattgaagc ggcacaacgc gccaccgcag aagccaaggc gctagcgatt
     1201 ttggacgcga gcctgccgag cgccctggac gcgtccggcg acctgatcga cggcgtgcga
     1261 gtgctctgga caaatccaga gcgagcgcgc gacgagaccg cgtttcgcca cgcgttgacc
     1321 gtgggatacc agctcaaagc tgctggtgag cgcctaaaag atgccaagat catcgacgcg
     1381 tatgaagtgg cgtacaacgt tgcccaggcg gtcggtgcag acggccggga gccggatctt
     1441 cccgccatgc gtgatcgcct gacgatggcg cgtcgtgtgc gcggctacgt ggctaaaggc
     1501 cagccagtcg tccctgctcg tcgggtggaa acgcagagca gccgagggcg gaaagctcta
     1561 gcgacgatgg ggcgacgggg cgcagctaca tcgaatgcac gcagatgggc tgacccagaa
     1621 agtaagtatg cgcaggagac gcgacagcga ttagcggaag caaacaaacg ccgagaaatg
     1681 acaggcgagt tgctcgaact tcgcgtcaaa actgcgatcc tggatgcccg ttctcaatcg
     1741 gttgctgatc cctcgactcg tgagcttgca ggcgaactag gtgtcagtga aaggcgcatc
     1801 caacaagtca gaaaggcact tggaatggaa gctaaacgcg gccgtccacg ggctgaaaac
     1861 taataaacga aacaccgtca gcagaaaacg gttcccccct ttaggggtcc cgtccttgct
     1921 ctggctctca cttgccctca ccctccgcta tccacgggct gaaaactaat aaacgaaaca
     1981 ccgtcagcag aaaacggttc ccccccttta gggtgtctcg ctcctagctc tgatccctcc
     2041 ccggttcctc cccggcctga tttttaaggg gggctcacgc tgtcggcaga gaacggttcc
     2101 ccgccttctg ctctggctct tcctcgactc cctccccctc aaaaatctcc tcgagatcct
     2161 ggagaccttt ttggagctag cgcgttgctg cttcgcacca acttgctcat gatgattttc
     2221 atttttgctt gtgtgctttt ttgggttgaa ccctccaaag aggggaaacc aggggcacac
     2281 ctcatgcact aaagtgccgc ttcgctggtc agggtgaaat cacctggaaa aaaagtgcgg
     2341 taaccgctgc gcttggcgtt ttttctgggc aagaagtctc gcaggttttc gcaggagtgc
     2401 cggaagaaat tatcagaatt ggggctagaa tttttaacga acgttcgtta taatggtgtc
     2461 atgaccttca cgacgaagta ccaaaactgg cctgaagcat cagcggtgga tctctccgat
     2521 gtcgcgctgg agtccgacgc actcgatgcc gccgtcgatt taaaaacggt gatcggattt
     2581 ttccgcgccc tcgatacgac agacgcgcca gcatcacgcg actgggcaag tgccgcgagc
     2641 gacctagaaa cgcttgtggc cgaccttgaa gagctggccg acgagctgcg tgctcggcag
     2701 cgccaggagg acgcgcagta gtggaggatc gcatcagctg cgcctactgc ggtggcctga
     2761 tcccaccccg gcctgaccca cgaggacggc gcgcaaaata ctgctcagac gcgtgtcgtg
     2821 ccgcagccag ccgcgagcgc gccaacaagc gccacgccca ggaggtcgaa gccgcacgtc
     2881 atctagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     2941 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     3001 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     3061 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     3121 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     3181 ggatctgatg gcgcagggga tcaagatcta tcgattttcg ttcgtgaata catgttataa
     3241 taactataac taataacgta acgtgactgg caagagatat ttttaaaaca atgaataggt
     3301 ttacacttac tttagtttta tggaaatgaa agatcatatc atatataatc tagaataaaa
     3361 ttaactaaaa taattattat ctagataaaa aatttagaag ccaatgaaat ctataaataa
     3421 actaaattaa gtttatttaa ttaacaacta tggatataaa ataggtacta atcaaaatag
     3481 tgaggaggat atatttgaat acatacgaac aaattaataa agtgaaaaaa atacttcgga
     3541 aacatttaaa aaataacctt attggtactt acatgtttgg atcaggagtt gagagtggac
     3601 taaaaccaaa tagtgatctt gactttttag tcgtcgtatc tgaaccattg acagatcaaa
     3661 gtaaagaaat acttatacaa aaaattagac ctatttcaaa aaaaatagga gataaaagca
     3721 acttacgata tattgaatta acaattatta ttcagcaaga aatggtaccg tggaatcatc
     3781 ctcccaaaca agaatttatt tatggagaat ggttacaaga gctttatgaa caaggataca
     3841 ttcctcagaa ggaattaaat tcagatttaa ccataatgct ttaccaagca aaacgaaaaa
     3901 ataaaagaat atacggaaat tatgacttag aggaattact acctgatatt ccattttctg
     3961 atgtgagaag agccattatg gattcgtcag aggaattaat agataattat caggatgatg
     4021 aaaccaactc tatattaact ttatgccgta tgattttaac tatggacacg ggtaaaatca
     4081 taccaaaaga tattgcggga aatgcagtgg ctgaatcttc tccattagaa catagggaga
     4141 gaattttgtt agcagttcgt agttatcttg gagagaatat tgaatggact aatgaaaatg
     4201 taaatttaac tataaactat ttaaataaca gattaaaaaa attataaaaa aattgaaaaa
     4261 atggtggaaa cacttttttc aatttttttg ttttattatt taatatttgg gaaatattca
     4321 ttctaattgg taatcagatt ttagaaaaca ataaaccctt gcatatgata tcgatgtaca
     4381 gatctgatcg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     4441 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgccccgc
     4501 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     4561 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     4621 gcagcggaaa agccatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5101 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5281 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5341 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5401 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5461 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5521 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5581 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5641 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5701 atgattacgc c
//
