LOCUS       40924_16665        5710 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pEC-S18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 5710)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             403..1866
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO66607.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            403..1866
                     /gene="repA"
                     /label=repA
     CDS             2509..2724
                     /codon_start=1
                     /gene="per"
                     /product="Per"
                     /label=per
                     /note="positive effector of replication"
                     /protein_id="AAO66608.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2509..2724
                     /gene="per"
                     /label=per
     CDS             3483..4250
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin adenylyltransferase"
                     /protein_id="AAO66609.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            3483..4250
                     /gene="aad9"
                     /label=aad9
     oriT            complement(4392..4500)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4712..5300
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5588..5609
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5624..5654
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5662..5678
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5686..5702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gataagctag atccccatca
      241 atcctgccta tttgccacgt ttaacaaggt agttaagcgt tcatttacga agaaaacacg
      301 ataagctgca caaatacctg aaaaagttga acgccccgtg agcgggaact cacagggcgt
      361 cggctaaccc ccagtcatca gctgggagaa agcactcaag acatgactct agccgatccg
      421 caggacacag tcacagctag cgcgtggaaa ttgtccgccg atctgttcga cacccacccc
      481 gaagctatgc gctgcggctc acgcggctgg acggcagaag atcgccgcga actgctcgct
      541 cacctgggac gcgaaagctt ccagggcagc aagacaagag atttcgcgag cgcctggatt
      601 aaaaacccgg ataccggcga aacccaacca aagctctacc gggctggctc aaaagcgctg
      661 acgcggtgcc agtacgttgc gctgacgcac gcgcaacatg ccgcggtgat cgtgcttgac
      721 atcgatgtgc ccagccacca ggccggcggg aagattgagc acgtaaaccc gcaggtctac
      781 gcgattttag agaaatgggc acgcctagaa aaagcgccgg cttggatcgg cgtgaatccg
      841 ctgagcggga aatgccagct catctggctc attgacccgg tgtatgccgc agcaggtaaa
      901 accagcccaa atatgcgcct gctggctgca acgacggaag aaatgactcg tgttttcggc
      961 gctgaccagg ctttttcgca taggctgagc cggtggccgc tgcacgtctc agacgatccg
     1021 acagcctata aatggcactg ccagcatgat cgtgtggatc ggctggccga cctaatggag
     1081 attgctcgaa cgatgaccgg atcacagaag ccgaaaaagt acattgagca ggacttttcc
     1141 agcggacgcg cccgcattga agcggcacaa cgcgccaccg cagaagccaa ggcgctagcg
     1201 attttggacg cgagcctgcc gagcgccctg gacgcgtccg gcgacctgat cgacggcgtg
     1261 cgagtgctct ggacaaatcc agagcgagcg cgcgacgaga ccgcgtttcg ccacgcgttg
     1321 accgtgggat accagctcaa agctgctggt gagcgcctaa aagatgccaa gatcatcgac
     1381 gcgtatgaag tggcgtacaa cgttgcccag gcggtcggtg cagacggccg ggagccggat
     1441 cttcccgcca tgcgtgatcg cctgacgatg gcgcgtcgtg tgcgcggcta cgtggctaaa
     1501 ggccagccag tcgtccctgc tcgtcgggtg gaaacgcaga gcagccgagg gcggaaagct
     1561 ctagcgacga tggggcgacg gggcgcagct acatcgaatg cacgcagatg ggctgaccca
     1621 gaaagtaagt atgcgcagga gacgcgacag cgattagcgg aagcaaacaa acgccgagaa
     1681 atgacaggcg agttgctcga acttcgcgtc aaaactgcga tcctggatgc ccgttctcaa
     1741 tcggttgctg atccctcgac tcgtgagctt gcaggcgaac taggtgtcag tgaaaggcgc
     1801 atccaacaag tcagaaaggc acttggaatg gaagctaaac gcggccgtcc acgggctgaa
     1861 aactaataaa cgaaacaccg tcagcagaaa acggttcccc cctttagggg tcccgtcctt
     1921 gctctggctc tcacttgccc tcaccctccg ctatccacgg gctgaaaact aataaacgaa
     1981 acaccgtcag cagaaaacgg ttccccccct ttagggtgtc tcgctcctag ctctgatccc
     2041 tccccggttc ctccccggcc tgatttttaa ggggggctca cgctgtcggc agagaacggt
     2101 tccccgcctt ctgctctggc tcttcctcga ctccctcccc ctcaaaaatc tcctcgagat
     2161 cctggagacc tttttggagc tagcgcgttg ctgcttcgca ccaacttgct catgatgatt
     2221 ttcatttttg cttgtgtgct tttttgggtt gaaccctcca aagaggggaa accaggggca
     2281 cacctcatgc actaaagtgc cgcttcgctg gtcagggtga aatcacctgg aaaaaaagtg
     2341 cggtaaccgc tgcgcttggc gttttttctg ggcaagaagt ctcgcaggtt ttcgcaggag
     2401 tgccggaaga aattatcaga attggggcta gaatttttaa cgaacgttcg ttataatggt
     2461 gtcatgacct tcacgacgaa gtaccaaaac tggcctgaag catcagcggt ggatctctcc
     2521 gatgtcgcgc tggagtccga cgcactcgat gccgccgtcg atttaaaaac ggtgatcgga
     2581 tttttccgcg ccctcgatac gacagacgcg ccagcatcac gcgactgggc aagtgccgcg
     2641 agcgacctag aaacgcttgt ggccgacctt gaagagctgg ccgacgagct gcgtgctcgg
     2701 cagcgccagg aggacgcgca gtagtggagg atcgcatcag ctgcgcctac tgcggtggcc
     2761 tgatcccacc ccggcctgac ccacgaggac ggcgcgcaaa atactgctca gacgcgtgtc
     2821 gtgccgcagc cagccgcgag cgcgccaaca agcgccacgc ccaggaggtc gaagccgcac
     2881 gtcatctagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga
     2941 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     3001 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     3061 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3121 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     3181 caaggatctg atggcgcagg ggatcaagat ctatcgattt tcgttcgtga atacatgtta
     3241 taataactat aactaataac gtaacgtgac tggcaagaga tatttttaaa acaatgaata
     3301 ggtttacact tactttagtt ttatggaaat gaaagatcat atcatatata atctagaata
     3361 aaattaacta aaataattat tatctagata aaaaatttag aagccaatga aatctataaa
     3421 taaactaaat taagtttatt taattaacaa ctatggatat aaaataggta ctaatcaaaa
     3481 tagtgaggag gatatatttg aatacatacg aacaaattaa taaagtgaaa aaaatacttc
     3541 ggaaacattt aaaaaataac cttattggta cttacatgtt tggatcagga gttgagagtg
     3601 gactaaaacc aaatagtgat cttgactttt tagtcgtcgt atctgaacca ttgacagatc
     3661 aaagtaaaga aatacttata caaaaaatta gacctatttc aaaaaaaata ggagataaaa
     3721 gcaacttacg atatattgaa ttaacaatta ttattcagca agaaatggta ccgtggaatc
     3781 atcctcccaa acaagaattt atttatggag aatggttaca agagctttat gaacaaggat
     3841 acattcctca gaaggaatta aattcagatt taaccataat gctttaccaa gcaaaacgaa
     3901 aaaataaaag aatatacgga aattatgact tagaggaatt actacctgat attccatttt
     3961 ctgatgtgag aagagccatt atggattcgt cagaggaatt aatagataat tatcaggatg
     4021 atgaaaccaa ctctatatta actttatgcc gtatgatttt aactatggac acgggtaaaa
     4081 tcataccaaa agatattgcg ggaaatgcag tggctgaatc ttctccatta gaacataggg
     4141 agagaatttt gttagcagtt cgtagttatc ttggagagaa tattgaatgg actaatgaaa
     4201 atgtaaattt aactataaac tatttaaata acagattaaa aaaattataa aaaaattgaa
     4261 aaaatggtgg aaacactttt ttcaattttt ttgttttatt atttaatatt tgggaaatat
     4321 tcattctaat tggtaatcag attttagaaa acaataaacc cttgcatatg atatcgatgt
     4381 acagatctga tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     4441 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     4501 cgctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     4561 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     4621 tatgcagcgg aaaagccatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4921 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4981 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5041 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5101 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5161 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5221 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5281 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5341 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5401 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5461 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5521 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5581 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5701 acatgattac
//
