LOCUS       40924_16660        5695 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pEC-K19mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 5695)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             400..1863
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO66604.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            400..1863
                     /gene="repA"
                     /label=repA
     CDS             2506..2721
                     /codon_start=1
                     /gene="per"
                     /product="Per"
                     /label=per
                     /note="positive effector of replication"
                     /protein_id="AAO66605.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2506..2721
                     /gene="per"
                     /label=per
     CDS             3240..4031
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     oriT            complement(4372..4481)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4693..5281
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5569..5590
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5605..5635
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5643..5659
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5667..5683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat aagctagatc cccatcaatc
      241 ctgcctattt gccacgttta acaaggtagt taagcgttca tttacgaaga aaacacgata
      301 agctgcacaa atacctgaaa aagttgaacg ccccgtgagc gggaactcac agggcgtcgg
      361 ctaaccccca gtcatcagct gggagaaagc actcaagaca tgactctagc cgatccgcag
      421 gacacagtca cagctagcgc gtggaaattg tccgccgatc tgttcgacac ccaccccgaa
      481 gctatgcgct gcggctcacg cggctggacg gcagaagatc gccgcgaact gctcgctcac
      541 ctgggacgcg aaagcttcca gggcagcaag acaagagatt tcgcgagcgc ctggattaaa
      601 aacccggata ccggcgaaac ccaaccaaag ctctaccggg ctggctcaaa agcgctgacg
      661 cggtgccagt acgttgcgct gacgcacgcg caacatgccg cggtgatcgt gcttgacatc
      721 gatgtgccca gccaccaggc cggcgggaag attgagcacg taaacccgca ggtctacgcg
      781 attttagaga aatgggcacg cctagaaaaa gcgccggctt ggatcggcgt gaatccgctg
      841 agcgggaaat gccagctcat ctggctcatt gacccggtgt atgccgcagc aggtaaaacc
      901 agcccaaata tgcgcctgct ggctgcaacg acggaagaaa tgactcgtgt tttcggcgct
      961 gaccaggctt tttcgcatag gctgagccgg tggccgctgc acgtctcaga cgatccgaca
     1021 gcctataaat ggcactgcca gcatgatcgt gtggatcggc tggccgacct aatggagatt
     1081 gctcgaacga tgaccggatc acagaagccg aaaaagtaca ttgagcagga cttttccagc
     1141 ggacgcgccc gcattgaagc ggcacaacgc gccaccgcag aagccaaggc gctagcgatt
     1201 ttggacgcga gcctgccgag cgccctggac gcgtccggcg acctgatcga cggcgtgcga
     1261 gtgctctgga caaatccaga gcgagcgcgc gacgagaccg cgtttcgcca cgcgttgacc
     1321 gtgggatacc agctcaaagc tgctggtgag cgcctaaaag atgccaagat catcgacgcg
     1381 tatgaagtgg cgtacaacgt tgcccaggcg gtcggtgcag acggccggga gccggatctt
     1441 cccgccatgc gtgatcgcct gacgatggcg cgtcgtgtgc gcggctacgt ggctaaaggc
     1501 cagccagtcg tccctgctcg tcgggtggaa acgcagagca gccgagggcg gaaagctcta
     1561 gcgacgatgg ggcgacgggg cgcagctaca tcgaatgcac gcagatgggc tgacccagaa
     1621 agtaagtatg cgcaggagac gcgacagcga ttagcggaag caaacaaacg ccgagaaatg
     1681 acaggcgagt tgctcgaact tcgcgtcaaa actgcgatcc tggatgcccg ttctcaatcg
     1741 gttgctgatc cctcgactcg tgagcttgca ggcgaactag gtgtcagtga aaggcgcatc
     1801 caacaagtca gaaaggcact tggaatggaa gctaaacgcg gccgtccacg ggctgaaaac
     1861 taataaacga aacaccgtca gcagaaaacg gttcccccct ttaggggtcc cgtccttgct
     1921 ctggctctca cttgccctca ccctccgcta tccacgggct gaaaactaat aaacgaaaca
     1981 ccgtcagcag aaaacggttc ccccccttta gggtgtctcg ctcctagctc tgatccctcc
     2041 ccggttcctc cccggcctga tttttaaggg gggctcacgc tgtcggcaga gaacggttcc
     2101 ccgccttctg ctctggctct tcctcgactc cctccccctc aaaaatctcc tcgagatcct
     2161 ggagaccttt ttggagctag cgcgttgctg cttcgcacca acttgctcat gatgattttc
     2221 atttttgctt gtgtgctttt ttgggttgaa ccctccaaag aggggaaacc aggggcacac
     2281 ctcatgcact aaagtgccgc ttcgctggtc agggtgaaat cacctggaaa aaaagtgcgg
     2341 taaccgctgc gcttggcgtt ttttctgggc aagaagtctc gcaggttttc gcaggagtgc
     2401 cggaagaaat tatcagaatt ggggctagaa tttttaacga acgttcgtta taatggtgtc
     2461 atgaccttca cgacgaagta ccaaaactgg cctgaagcat cagcggtgga tctctccgat
     2521 gtcgcgctgg agtccgacgc actcgatgcc gccgtcgatt taaaaacggt gatcggattt
     2581 ttccgcgccc tcgatacgac agacgcgcca gcatcacgcg actgggcaag tgccgcgagc
     2641 gacctagaaa cgcttgtggc cgaccttgaa gagctggccg acgagctgcg tgctcggcag
     2701 cgccaggagg acgcgcagta gtggaggatc gcatcagctg cgcctactgc ggtggcctga
     2761 tcccaccccg gcctgaccca cgaggacggc gcgcaaaata ctgctcagac gcgtgtcgtg
     2821 ccgcagccag ccgcgagcgc gccaacaagc gccacgccca ggaggtcgaa gccgcacgtc
     2881 atctagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     2941 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     3001 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     3061 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     3121 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     3181 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     3241 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3301 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3361 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
     3421 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3481 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3541 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3601 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3661 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3721 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg
     3781 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3841 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3901 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3961 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4021 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     4081 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4141 tttccgggac gccctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct
     4201 ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa
     4261 tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg
     4321 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
     4381 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
     4441 aacacccgct cgcgggtggg cctacttcac ctatcctgcc ccgctgacgc cgttggatac
     4501 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     4561 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagccat
     4621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4801 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     5101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5341 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5401 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5461 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5521 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5581 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5641 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgcc
//
