LOCUS       40924_16650        5695 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEC-K18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Efficient electrotransformation of corynebacterium diphtheriae with 
            a mini-replicon derived from the Corynebacterium glutamicum plasmid 
            pGA1
  JOURNAL   Curr. Microbiol. 45 (5), 362-367 (2002)
  PUBMED    12232668
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2001) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 5695)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2002"; volume: "45"; issue: "5"; pages: 
            "362-367"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2001) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             184..1647
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /note="Rep"
                     /protein_id="AAL38580.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            184..1647
                     /gene="rep"
                     /label=rep
     CDS             2290..2505
                     /codon_start=1
                     /gene="per"
                     /product="positive effector of replication"
                     /label=per
                     /note="Per"
                     /protein_id="AAL38581.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2290..2505
                     /gene="per"
                     /label=per
     CDS             3024..3815
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     oriT            complement(4156..4265)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4477..5065
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5353..5374
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5389..5419
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5427..5443
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5451..5467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5477..5533
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5537..5553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgataagcta gatccccatc aatcctgcct atttgccacg tttaacaagg tagttaagcg
       61 ttcatttacg aagaaaacac gataagctgc acaaatacct gaaaaagttg aacgccccgt
      121 gagcgggaac tcacagggcg tcggctaacc cccagtcatc agctgggaga aagcactcaa
      181 gacatgactc tagccgatcc gcaggacaca gtcacagcta gcgcgtggaa attgtccgcc
      241 gatctgttcg acacccaccc cgaagctatg cgctgcggct cacgcggctg gacggcagaa
      301 gatcgccgcg aactgctcgc tcacctggga cgcgaaagct tccagggcag caagacaaga
      361 gatttcgcga gcgcctggat taaaaacccg gataccggcg aaacccaacc aaagctctac
      421 cgggctggct caaaagcgct gacgcggtgc cagtacgttg cgctgacgca cgcgcaacat
      481 gccgcggtga tcgtgcttga catcgatgtg cccagccacc aggccggcgg gaagattgag
      541 cacgtaaacc cgcaggtcta cgcgatttta gagaaatggg cacgcctaga aaaagcgccg
      601 gcttggatcg gcgtgaatcc gctgagcggg aaatgccagc tcatctggct cattgacccg
      661 gtgtatgccg cagcaggtaa aaccagccca aatatgcgcc tgctggctgc aacgacggaa
      721 gaaatgactc gtgttttcgg cgctgaccag gctttttcgc ataggctgag ccggtggccg
      781 ctgcacgtct cagacgatcc gacagcctat aaatggcact gccagcatga tcgtgtggat
      841 cggctggccg acctaatgga gattgctcga acgatgaccg gatcacagaa gccgaaaaag
      901 tacattgagc aggacttttc cagcggacgc gcccgcattg aagcggcaca acgcgccacc
      961 gcagaagcca aggcgctagc gattttggac gcgagcctgc cgagcgccct ggacgcgtcc
     1021 ggcgacctga tcgacggcgt gcgagtgctc tggacaaatc cagagcgagc gcgcgacgag
     1081 accgcgtttc gccacgcgtt gaccgtggga taccagctca aagctgctgg tgagcgccta
     1141 aaagatgcca agatcatcga cgcgtatgaa gtggcgtaca acgttgccca ggcggtcggt
     1201 gcagacggcc gggagccgga tcttcccgcc atgcgtgatc gcctgacgat ggcgcgtcgt
     1261 gtgcgcggct acgtggctaa aggccagcca gtcgtccctg ctcgtcgggt ggaaacgcag
     1321 agcagccgag ggcggaaagc tctagcgacg atggggcgac ggggcgcagc tacatcgaat
     1381 gcacgcagat gggctgaccc agaaagtaag tatgcgcagg agacgcgaca gcgattagcg
     1441 gaagcaaaca aacgccgaga aatgacaggc gagttgctcg aacttcgcgt caaaactgcg
     1501 atcctggatg cccgttctca atcggttgct gatccctcga ctcgtgagct tgcaggcgaa
     1561 ctaggtgtca gtgaaaggcg catccaacaa gtcagaaagg cacttggaat ggaagctaaa
     1621 cgcggccgtc cacgggctga aaactaataa acgaaacacc gtcagcagaa aacggttccc
     1681 ccctttaggg gtcccgtcct tgctctggct ctcacttgcc ctcaccctcc gctatccacg
     1741 ggctgaaaac taataaacga aacaccgtca gcagaaaacg gttccccccc tttagggtgt
     1801 ctcgctccta gctctgatcc ctccccggtt cctccccggc ctgattttta aggggggctc
     1861 acgctgtcgg cagagaacgg ttccccgcct tctgctctgg ctcttcctcg actccctccc
     1921 cctcaaaaat ctcctcgaga tcctggagac ctttttggag ctagcgcgtt gctgcttcgc
     1981 accaacttgc tcatgatgat tttcattttt gcttgtgtgc ttttttgggt tgaaccctcc
     2041 aaagagggga aaccaggggc acacctcatg cactaaagtg ccgcttcgct ggtcagggtg
     2101 aaatcacctg gaaaaaaagt gcggtaaccg ctgcgcttgg cgttttttct gggcaagaag
     2161 tctcgcaggt tttcgcagga gtgccggaag aaattatcag aattggggct agaattttta
     2221 acgaacgttc gttataatgg tgtcatgacc ttcacgacga agtaccaaaa ctggcctgaa
     2281 gcatcagcgg tggatctctc cgatgtcgcg ctggagtccg acgcactcga tgccgccgtc
     2341 gatttaaaaa cggtgatcgg atttttccgc gccctcgata cgacagacgc gccagcatca
     2401 cgcgactggg caagtgccgc gagcgaccta gaaacgcttg tggccgacct tgaagagctg
     2461 gccgacgagc tgcgtgctcg gcagcgccag gaggacgcgc agtagtggag gatcgcatca
     2521 gctgcgccta ctgcggtggc ctgatcccac cccggcctga cccacgagga cggcgcgcaa
     2581 aatactgctc agacgcgtgt cgtgccgcag ccagccgcga gcgcgccaac aagcgccacg
     2641 cccaggaggt cgaagccgca cgtcatctag cttcacgctg ccgcaagcac tcagggcgca
     2701 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     2761 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     2821 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     2881 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     2941 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
     3001 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     3061 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3121 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3181 tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg gctatcgtgg ctggccacga
     3241 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3301 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3361 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3421 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3481 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3541 ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3601 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3661 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3721 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3781 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     3841 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccctc gcggacgtgc tcatagtcca
     3961 cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc
     4021 cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga
     4081 taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
     4141 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     4201 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     4261 tgccccgctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
     4321 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
     4381 agggttatgc agcggaaaag ccatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4621 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4861 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5221 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5281 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5341 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5401 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5461 ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     5521 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     5581 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     5641 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcg
//
