LOCUS       40924_16645        5498 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pEC-C18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 5498)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5498)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             403..1866
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein"
                     /protein_id="AAO66601.1"
                     /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL
                     AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV
                     LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA
                     AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA
                     DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD
                     LIDGVRVLWTNPERARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVGA
                     DGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATSN
                     ARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELAG
                     ELGVSERRIQQVRKALGMEAKRGRPRAEN"
     gene            403..1866
                     /gene="repA"
                     /label=repA
     CDS             2509..2724
                     /codon_start=1
                     /gene="per"
                     /product="Per"
                     /label=per
                     /note="positive effector of replication"
                     /protein_id="AAO66602.1"
                     /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS
                     DLETLVADLEELADELRARQRQEDAQ"
     gene            2509..2724
                     /gene="per"
                     /label=per
     promoter        3151..3253
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3254..3910
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            complement(4180..4288)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4500..5088
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5376..5397
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5412..5442
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5450..5466
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5474..5490
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gataagctag atccccatca
      241 atcctgccta tttgccacgt ttaacaaggt agttaagcgt tcatttacga agaaaacacg
      301 ataagctgca caaatacctg aaaaagttga acgccccgtg agcgggaact cacagggcgt
      361 cggctaaccc ccagtcatca gctgggagaa agcactcaag acatgactct agccgatccg
      421 caggacacag tcacagctag cgcgtggaaa ttgtccgccg atctgttcga cacccacccc
      481 gaagctatgc gctgcggctc acgcggctgg acggcagaag atcgccgcga actgctcgct
      541 cacctgggac gcgaaagctt ccagggcagc aagacaagag atttcgcgag cgcctggatt
      601 aaaaacccgg ataccggcga aacccaacca aagctctacc gggctggctc aaaagcgctg
      661 acgcggtgcc agtacgttgc gctgacgcac gcgcaacatg ccgcggtgat cgtgcttgac
      721 atcgatgtgc ccagccacca ggccggcggg aagattgagc acgtaaaccc gcaggtctac
      781 gcgattttag agaaatgggc acgcctagaa aaagcgccgg cttggatcgg cgtgaatccg
      841 ctgagcggga aatgccagct catctggctc attgacccgg tgtatgccgc agcaggtaaa
      901 accagcccaa atatgcgcct gctggctgca acgacggaag aaatgactcg tgttttcggc
      961 gctgaccagg ctttttcgca taggctgagc cggtggccgc tgcacgtctc agacgatccg
     1021 acagcctata aatggcactg ccagcatgat cgtgtggatc ggctggccga cctaatggag
     1081 attgctcgaa cgatgaccgg atcacagaag ccgaaaaagt acattgagca ggacttttcc
     1141 agcggacgcg cccgcattga agcggcacaa cgcgccaccg cagaagccaa ggcgctagcg
     1201 attttggacg cgagcctgcc gagcgccctg gacgcgtccg gcgacctgat cgacggcgtg
     1261 cgagtgctct ggacaaatcc agagcgagcg cgcgacgaga ccgcgtttcg ccacgcgttg
     1321 accgtgggat accagctcaa agctgctggt gagcgcctaa aagatgccaa gatcatcgac
     1381 gcgtatgaag tggcgtacaa cgttgcccag gcggtcggtg cagacggccg ggagccggat
     1441 cttcccgcca tgcgtgatcg cctgacgatg gcgcgtcgtg tgcgcggcta cgtggctaaa
     1501 ggccagccag tcgtccctgc tcgtcgggtg gaaacgcaga gcagccgagg gcggaaagct
     1561 ctagcgacga tggggcgacg gggcgcagct acatcgaatg cacgcagatg ggctgaccca
     1621 gaaagtaagt atgcgcagga gacgcgacag cgattagcgg aagcaaacaa acgccgagaa
     1681 atgacaggcg agttgctcga acttcgcgtc aaaactgcga tcctggatgc ccgttctcaa
     1741 tcggttgctg atccctcgac tcgtgagctt gcaggcgaac taggtgtcag tgaaaggcgc
     1801 atccaacaag tcagaaaggc acttggaatg gaagctaaac gcggccgtcc acgggctgaa
     1861 aactaataaa cgaaacaccg tcagcagaaa acggttcccc cctttagggg tcccgtcctt
     1921 gctctggctc tcacttgccc tcaccctccg ctatccacgg gctgaaaact aataaacgaa
     1981 acaccgtcag cagaaaacgg ttccccccct ttagggtgtc tcgctcctag ctctgatccc
     2041 tccccggttc ctccccggcc tgatttttaa ggggggctca cgctgtcggc agagaacggt
     2101 tccccgcctt ctgctctggc tcttcctcga ctccctcccc ctcaaaaatc tcctcgagat
     2161 cctggagacc tttttggagc tagcgcgttg ctgcttcgca ccaacttgct catgatgatt
     2221 ttcatttttg cttgtgtgct tttttgggtt gaaccctcca aagaggggaa accaggggca
     2281 cacctcatgc actaaagtgc cgcttcgctg gtcagggtga aatcacctgg aaaaaaagtg
     2341 cggtaaccgc tgcgcttggc gttttttctg ggcaagaagt ctcgcaggtt ttcgcaggag
     2401 tgccggaaga aattatcaga attggggcta gaatttttaa cgaacgttcg ttataatggt
     2461 gtcatgacct tcacgacgaa gtaccaaaac tggcctgaag catcagcggt ggatctctcc
     2521 gatgtcgcgc tggagtccga cgcactcgat gccgccgtcg atttaaaaac ggtgatcgga
     2581 tttttccgcg ccctcgatac gacagacgcg ccagcatcac gcgactgggc aagtgccgcg
     2641 agcgacctag aaacgcttgt ggccgacctt gaagagctgg ccgacgagct gcgtgctcgg
     2701 cagcgccagg aggacgcgca gtagtggagg atcgcatcag ctgcgcctac tgcggtggcc
     2761 tgatcccacc ccggcctgac ccacgaggac ggcgcgcaaa atactgctca gacgcgtgtc
     2821 gtgccgcagc cagccgcgag cgcgccaaca agcgccacgc ccaggaggtc gaagccgcac
     2881 gtcatctagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct
     2941 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
     3001 acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt
     3061 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc
     3121 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca
     3181 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     3241 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     3301 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     3361 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     3421 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     3481 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     3541 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     3601 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     3661 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     3721 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     3781 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg
     3841 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     3901 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc
     3961 tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg
     4021 tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat
     4081 tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg
     4141 ctctccccgt ggaggtaata attgacgata tgatctgatc ggccagcctc gcagagcagg
     4201 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     4261 gggtgggcct acttcaccta tcctgccccg ctgacgccgt tggatacacc aaggaaagtc
     4321 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     4381 aatcgctata atgaccccga agcagggtta tgcagcggaa aagccatgac caaaatccct
     4441 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4501 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4561 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4621 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4681 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4741 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4801 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4861 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     4921 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4981 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5041 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5101 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5161 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5221 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5281 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5341 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5401 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5461 taacaatttc acacaggaaa cagctatgac atgattac
//
