LOCUS       V007669                 7716 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007669
VERSION     V007669
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Lynd L.
  TITLE     The identification of four histidine kinases that influence
            sporulation in Clostridium thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Mearls E.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 7716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-OCT-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      108..355
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(603..1191)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1365..2222)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(2321..3121)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3122..3342)
                     /label="URA3 promoter"
     misc_feature    3370..3873
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(4105..5106)
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      5592..6239
                     /label="gapD promoter"
                     /note="gapD promoter"
                     /regulatory_class="promoter"
     CDS             6240..6887
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             6906..7715
                     /codon_start=1
                     /product="Spo0A"
                     /label="Spo0A"
                     /note="master regulator of sporulation"
                     /protein_id="AHC95143.1"
                     /translation="MTSNKIRVVIADDNREFGDILYEYLNNQEDIEVVGVARDGIEAYE
                     LIVEKLPDIAILDIIMPHLDGLGVLEKIGATAISKRPLFIILSAVGQDKITQRALALGA
                     EYYVVKPFDMEVLISRIRQLKNVNQPNVIRQDGLSGEVKSSYHPPQPKNLEAEVTNIMH
                     EIGVPAHIKGYQYLRDAIIMVVKDLDVINSITKQLYPTIAKEYNTTPSRVERAIRHAIE
                     VAWSRGQIDTIDSLFGYTINVGKGKPTNSEFIAMVADKLRLEMKMAT"
ORIGIN
        1 ccgggcctcg agaaaacaaa aggctcagtc ggaagactgg gccttttgtt ttggtaccga
       61 attcggcgcg cctcagcgtt taaacccgct gatcctagag ggccgcatca tgtaattagt
      121 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
      181 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
      241 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
      301 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct
      361 gcggccctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      541 aacatgtgag caaaaggcca gcaaaagccc aggaaccgta aaaaggccgc gttgctggcg
      601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1021 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     1081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1441 gtgtagataa ctacgatacg ggagcgctta ccatctggcc ccagtgctgc aatgataccg
     1501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca
     1681 ggcatcgtgg tgtcactctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1981 cgggataata gtgtatcaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2221 atactcttcc tttttcaatg ggtaataact gatataatta aattgaagct ctaatttgtg
     2281 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc
     2341 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc
     2401 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat
     2461 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg
     2521 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc
     2581 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag
     2641 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg
     2701 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg
     2761 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga
     2821 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg
     2881 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat
     2941 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg
     3001 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata
     3061 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca
     3121 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc
     3181 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     3241 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa
     3301 atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc ttatcgatgg
     3361 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
     3421 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
     3481 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
     3541 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
     3601 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
     3661 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
     3721 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
     3781 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
     3841 tacgaggcgc gtgtaagtta caggcaagcg atccgtccgc cggcgaacgt ggcgagaaag
     3901 gaagggaaga aagcgaaagg agcgggggct agggcggtgg gaagtgtagg ggtcacgctg
     3961 ggcgtaacca ccacacccgc cgcgcttaat ggggcgctac agggcgcgtg gggatgatcc
     4021 actagtgaat ttaggaggct tacttgtctg ctttcttcat tagaatcaat ccttttttaa
     4081 aagtcaatcc cgtttgttga actactcttt aataaaataa tttttccgtt cccaattcca
     4141 cattgcaata atagaaaatc catcttcatc ggctttttcg tcatcatctg tatgaatcaa
     4201 atcgccttct tctgtgtcat caaggtttaa ttttttatgt atttctttta acaaaccacc
     4261 ataggagatt aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt
     4321 cttttcttca tcatcggtca taaaatccgt atcctttaca ggatattttg cagtttcgtc
     4381 aattgccgat tgtatatccg atttatattt atttttcggt cgaatcattt gaacttttac
     4441 atttggatca tagtctaatt tcattgcctt tttccaaaat tgaatccatt gtttttgatt
     4501 cacgtagttt tctgtattct taaaataagt tggttccaca cataccaata catgcatgtg
     4561 ctgattataa gaattatctt tattatttat tgtcacttcc gttgcacgca taaaaccaac
     4621 aagattttta ttaatttttt tatattgcat cattcggcga aatccttgag ccatatctga
     4681 caaactctta tttaattctt cgccatcata aacattttta actgttaatg tgagaaacaa
     4741 ccaacgaact gttggctttt gtttaataac ttcagcaaca accttttgtg actgaatgcc
     4801 atgtttcatt gctctcctcc agttgcacat tggacaaagc ctggatttac aaaaccacac
     4861 tcgatacaac tttctttcgc ctgtttcacg attttgttta tactctaata tttcagcaca
     4921 atcttttact ctttcagcct ttttaaattc aagaatatgc agaagttcaa agtaatcaac
     4981 attagcgatt ttcttttctc tccatggtct cacttttcca ctttttgtct tgtccactaa
     5041 aacccttgat ttttcatctg aataaatgct actattagga cacataatat taaaagaaac
     5101 ccccatctat ttagttattt gtttggtcac ttataacttt aacagatggg gtttttctgt
     5161 gcaaccaatt ttaagggttt tcaatacttt aaaacacata cataccaaca cttcaacgca
     5221 cctttcagca actaaaataa aaatgacgtt atttctatat gtatcaagaa tagaaagaac
     5281 tcgtttttcg ctacgctcaa aacgcaaaaa aagcactcat tcgagtgctt tttcttatcg
     5341 ctccaaatca tgcgattttt tcctctttgc ttttctttgc tcacgaagtt ctcgatcacg
     5401 ctgcaaaaca tcttgaagcg aaaaagtatt cttcttttct tccgatcgct catgctgacg
     5461 cacgaaaagc cctctaggcg cataggaaca actcctaaat gcatgtgagg ggttttctcg
     5521 tccatgtgaa cagtcgcata cgcaatattt tgtttcccat agctagtaag cttggatcct
     5581 cgcgaggccg gccagtattc tgacatgggt gtatcaataa cccatgcgtt tccgtattgt
     5641 atcggaatgg tttcggacag ggcggtggga atagacatgg aaaagatttt tttgcccgag
     5701 gatgcattga taaagtattt cttttccgaa agagaggaaa agattctaaa gagttttgga
     5761 aatactgatg aatattgtgt gcagagtaca attctatgga caagaaaaga ggctttgtca
     5821 aaactttttc gtctgggaat gaggatggat tttaaaaagc tggatacttt ggaggacgag
     5881 gtggtttttc aggaaacaaa cagggcgcgt ctgttttctt ttatatgcaa taattactgt
     5941 atctctctgg cattgccagg ttttaataaa gattaaaatt attgactaga aataaaaaaa
     6001 ttgtccataa tattaatgga caaaaaaaca aagaattaca tcaaaggaag ataaaaatac
     6061 tttgttaaaa aattaattat tttttatcta aactattgaa aatgaaaata aaataatata
     6121 aaatgaatca tagtgcaaga gatacttgcc agaggatgaa tattttactg cattcatgct
     6181 ttatggcagc taatagaggc attaaattaa attttaattt acaataggag gcgatattaa
     6241 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     6301 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     6361 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     6421 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     6481 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     6541 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     6601 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     6661 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     6721 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     6781 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     6841 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa taaaggaggt
     6901 cgacgttgac atcaaataag attcgggtcg taattgctga tgataacagg gaatttggcg
     6961 atattttata tgaatacctt aacaatcagg aagatattga agtggtaggg gtggcaagag
     7021 acggtattga agcttatgag cttatagttg aaaaacttcc tgacattgct atacttgata
     7081 ttatcatgcc tcatttggac ggtttgggtg ttttggagaa aataggcgcg actgcaatca
     7141 gtaaaagacc tttgttcatt attctttcgg cagtgggcca ggacaagata acacaaagag
     7201 cattggcact cggagctgaa tactatgttg taaagccttt cgacatggaa gttttaattt
     7261 cgagaataag acaactcaag aacgtgaacc agcccaatgt tataagacag gacggtttgt
     7321 ccggtgaagt caagtcgtct tatcatcctc cgcagccaaa aaatcttgaa gctgaagtaa
     7381 caaatattat gcatgaaata ggcgtaccgg cccatataaa agggtatcag tatctgaggg
     7441 atgccataat aatggtagtc aaagaccttg acgtgataaa ttcaattaca aagcagctgt
     7501 atcctacaat agcgaaggaa tacaacacca cacccagcag agttgaaagg gcaataagac
     7561 atgctattga agtggcgtgg agcaggggac agattgatac aatagattcg ttgtttggct
     7621 atacgataaa tgttggaaaa ggtaaaccca caaattcgga atttatagca atggtggcag
     7681 acaaattgag attggaaatg aaaatggcta cataaa
//
