LOCUS       V007671                 8709 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007671
VERSION     V007671
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Lynd LR.
  TITLE     Identification of sporulation histidine kinases in C. thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      97..344
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(592..1180)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1354..2211)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(2310..3110)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3111..3331)
                     /label="URA3 promoter"
     misc_feature    3359..3862
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(4094..5095)
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      5581..6228
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            5581..6228
                     /gene="gapD"
                     /label="gapD"
     CDS             6229..6876
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             6895..8709
                     /codon_start=1
                     /product="histidine kinase"
                     /label="histidine kinase"
                     /note="from Clostridium thermocellum Clo1313_0495"
                     /protein_id="AGO62092.1"
                     /translation="MLFGRKKGMPEVIKGLEEEKISTSWKLNLEFALIVMFSIFVVGAA
                     SFGMISNFILNHAKVSSSELIKQTSKNIEAILTGFDELAMTVSRDNTLAEYISVHDSIE
                     DVNLKAQNERKIKEILNNYAKKRKDITNIAVVSNGGTYITPDETKPGIDKNIDDIYAVK
                     AFKESHRQSLWLNTYTLDTSPSENVQVFSIIKGIYSLSSLKSQGILIINITEDYLFRLI
                     SDIKPIDDGRIYIIGSDGNYVLNPYDRSKNGKKADLEFVEDMLRKGENVDIKEINGEEY
                     LVTYNTIQEIKGTGLGWMIVEITPVSVIRTSVTEAGMRLFFIGFGCVVLGLILVGMATA
                     FYNRYLNKSYWERHSVALERERLASLGQLIGGIAHNFKTPIMSIAGGLEALKDLVDEYD
                     ISIGDPQVTGEDHHEIAAEMRDWISKIKPYCGYMSEIISTVKGQADNMNGSENSSFTVG
                     ELLKRVEILMSHELKKFSCELRLDIKVDEDTTIKGEINNLVQVLNNLISNSIESYNGKE
                     GKIDLSVSKNGQELEIVVKDYGCGIPENVKRKLLKEMITTKGKNGTGLGLYMSHSTIKG
                     KFGGTMKVKSEEGKGTEICILIPFAAKT"
ORIGIN
        1 gaaaacaaaa ggctcagtcg gaagactggg ccttttgttt tggtaccgaa ttcggcgcgc
       61 ctcagcgttt aaacccgctg atcctagagg gccgcatcat gtaattagtt atgtcacgct
      121 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
      181 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
      241 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
      301 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctg cggccctgca
      361 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      421 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      481 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      541 aaaaggccag caaaagccca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1021 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1441 tacgatacgg gagcgcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1621 aagtagttcg ccagttaata gtttgcgcaa cgttgttggc attgctacag gcatcgtggt
     1681 gtcactctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatag
     1981 tgtatcacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2221 ttttcaatgg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     2281 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     2341 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     2401 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
     2461 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     2521 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     2581 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     2641 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     2701 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     2761 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     2821 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     2881 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     2941 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     3001 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     3061 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     3121 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     3181 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     3241 tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa
     3301 gaataaaaaa aaaatgatga attgaattga aaagctagct tatcgatggg tccttttcat
     3361 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
     3421 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
     3481 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
     3541 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta
     3601 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
     3661 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
     3721 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
     3781 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
     3841 tgtaagttac aggcaagcga tccgtccgcc ggcgaacgtg gcgagaaagg aagggaagaa
     3901 agcgaaagga gcgggggcta gggcggtggg aagtgtaggg gtcacgctgg gcgtaaccac
     3961 cacacccgcc gcgcttaatg gggcgctaca gggcgcgtgg ggatgatcca ctagtgaatt
     4021 taggaggctt acttgtctgc tttcttcatt agaatcaatc cttttttaaa agtcaatccc
     4081 gtttgttgaa ctactcttta ataaaataat ttttccgttc ccaattccac attgcaataa
     4141 tagaaaatcc atcttcatcg gctttttcgt catcatctgt atgaatcaaa tcgccttctt
     4201 ctgtgtcatc aaggtttaat tttttatgta tttcttttaa caaaccacca taggagatta
     4261 accttttacg gtgtaaacct tcctccaaat cagacaaacg tttcaaattc ttttcttcat
     4321 catcggtcat aaaatccgta tcctttacag gatattttgc agtttcgtca attgccgatt
     4381 gtatatccga tttatattta tttttcggtc gaatcatttg aacttttaca tttggatcat
     4441 agtctaattt cattgccttt ttccaaaatt gaatccattg tttttgattc acgtagtttt
     4501 ctgtattctt aaaataagtt ggttccacac ataccaatac atgcatgtgc tgattataag
     4561 aattatcttt attatttatt gtcacttccg ttgcacgcat aaaaccaaca agatttttat
     4621 taattttttt atattgcatc attcggcgaa atccttgagc catatctgac aaactcttat
     4681 ttaattcttc gccatcataa acatttttaa ctgttaatgt gagaaacaac caacgaactg
     4741 ttggcttttg tttaataact tcagcaacaa ccttttgtga ctgaatgcca tgtttcattg
     4801 ctctcctcca gttgcacatt ggacaaagcc tggatttaca aaaccacact cgatacaact
     4861 ttctttcgcc tgtttcacga ttttgtttat actctaatat ttcagcacaa tcttttactc
     4921 tttcagcctt tttaaattca agaatatgca gaagttcaaa gtaatcaaca ttagcgattt
     4981 tcttttctct ccatggtctc acttttccac tttttgtctt gtccactaaa acccttgatt
     5041 tttcatctga ataaatgcta ctattaggac acataatatt aaaagaaacc cccatctatt
     5101 tagttatttg tttggtcact tataacttta acagatgggg tttttctgtg caaccaattt
     5161 taagggtttt caatacttta aaacacatac ataccaacac ttcaacgcac ctttcagcaa
     5221 ctaaaataaa aatgacgtta tttctatatg tatcaagaat agaaagaact cgtttttcgc
     5281 tacgctcaaa acgcaaaaaa agcactcatt cgagtgcttt ttcttatcgc tccaaatcat
     5341 gcgatttttt cctctttgct tttctttgct cacgaagttc tcgatcacgc tgcaaaacat
     5401 cttgaagcga aaaagtattc ttcttttctt ccgatcgctc atgctgacgc acgaaaagcc
     5461 ctctaggcgc ataggaacaa ctcctaaatg catgtgaggg gttttctcgt ccatgtgaac
     5521 agtcgcatac gcaatatttt gtttcccata gctagtaagc ttggatcctc gcgaggccgg
     5581 ccagtattct gacatgggtg tatcaataac ccatgcgttt ccgtattgta tcggaatggt
     5641 ttcggacagg gcggtgggaa tagacatgga aaagattttt ttgcccgagg atgcattgat
     5701 aaagtatttc ttttccgaaa gagaggaaaa gattctaaag agttttggaa atactgatga
     5761 atattgtgtg cagagtacaa ttctatggac aagaaaagag gctttgtcaa aactttttcg
     5821 tctgggaatg aggatggatt ttaaaaagct ggatactttg gaggacgagg tggtttttca
     5881 ggaaacaaac agggcgcgtc tgttttcttt tatatgcaat aattactgta tctctctggc
     5941 attgccaggt tttaataaag attaaaatta ttgactagaa ataaaaaaat tgtccataat
     6001 attaatggac aaaaaaacaa agaattacat caaaggaaga taaaaatact ttgttaaaaa
     6061 attaattatt ttttatctaa actattgaaa atgaaaataa aataatataa aatgaatcat
     6121 agtgcaagag atacttgcca gaggatgaat attttactgc attcatgctt tatggcagct
     6181 aatagaggca ttaaattaaa ttttaattta caataggagg cgatattaat gaactttaat
     6241 aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa
     6301 acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa
     6361 gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat
     6421 acagctttta gaactggtta caatagcgac ggagagttag gttattggga taagttagag
     6481 ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta
     6541 aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt
     6601 tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt
     6661 ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta
     6721 cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct
     6781 ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt
     6841 caggaattgt cagataggcc taatgactgg cttttataat aaaggaggtc gacgatgctt
     6901 tttggacgca aaaagggaat gcccgaagtg ataaagggac ttgaagagga aaaaatcagc
     6961 acgtcatgga aattaaacct tgaatttgcg ctaatagtta tgttttccat atttgtggta
     7021 ggtgcagcct cgtttggtat gatttcgaac tttatattaa atcatgcaaa ggtcagttcc
     7081 agcgagctta tcaagcaaac atccaaaaac attgaagcta ttctgacagg ttttgatgaa
     7141 ctggcgatga ctgtgtcaag ggataacaca ctggcagagt atataagtgt gcacgacagt
     7201 atagaggatg ttaatcttaa ggctcaaaat gaaagaaaaa tcaaggaaat cctgaataat
     7261 tacgcaaaaa aaagaaaaga cataaccaat attgcagtgg tatcaaacgg gggaacttac
     7321 ataacaccag atgagacaaa accgggcatt gataaaaata tagatgacat ttatgcggta
     7381 aaagcgttta aagaaagcca caggcagtca ttatggctca acacgtatac attggacact
     7441 tcaccctccg aaaatgtaca ggtcttttca ataataaagg ggatatattc tttaagcagt
     7501 ctgaaaagcc agggaatctt gattataaac attacggaag actatctttt cagacttata
     7561 tcggatatta agcccattga cgacggaaga atctatataa tcggcagtga cgggaattac
     7621 gttttaaacc cctatgacag aagcaagaac ggtaaaaagg cggatcttga gtttgtggag
     7681 gacatgctgc gcaagggcga gaatgtggac ataaaggaaa taaatggtga ggagtatctt
     7741 gtgacttaca ataccattca ggagataaaa ggtaccgggc tgggatggat gatagtcgag
     7801 attactccgg tttcggtgat cagaaccagt gttaccgaag cgggaatgcg cctgtttttc
     7861 ataggttttg ggtgtgttgt cctgggattg attcttgtag ggatggcaac cgctttttac
     7921 aaccgatatc tcaataaaag ctattgggaa aggcattccg ttgcattgga aagggagaga
     7981 cttgcttcct tgggacaact gataggggga attgcacaca atttcaaaac tccaattatg
     8041 tcaatagccg gaggactgga ggcattaaaa gatcttgtgg atgagtacga catttccatc
     8101 ggagatccgc aggtaaccgg tgaggatcat catgaaattg ctgctgaaat gagagattgg
     8161 ataagcaaaa taaagcctta ctgcgggtat atgtcggaga ttatatccac ggtaaaaggg
     8221 caggccgaca atatgaatgg atcagagaat tcaagcttta cggtgggaga acttttaaaa
     8281 agagttgaaa ttttaatgag ccacgagctt aaaaaatttt cctgcgagct gagattggat
     8341 ataaaagtgg atgaggatac aactataaaa ggagaaataa acaaccttgt acaggtattg
     8401 aacaacctta tatccaattc tatcgagtct tacaacggaa aggaaggaaa aatagacctg
     8461 tcagtaagca aaaatggcca ggaattggaa atagttgtaa aagactatgg atgcggcata
     8521 ccggaaaatg taaagaggaa acttctgaaa gaaatgataa cgaccaaggg aaaaaacgga
     8581 acaggacttg gcctttatat gtctcactcc acgattaagg gcaaatttgg cggaacaatg
     8641 aaagtcaaga gtgaggaagg aaaagggacg gaaatatgca ttttgattcc ttttgctgcc
     8701 aaaacttaa
//
