LOCUS       V007682                12613 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007682
VERSION     V007682
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Lynd LR.
  TITLE     Identification of sporulation histidine kinases in C. thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2139
                     /codon_start=1
                     /product="histidine kinase"
                     /label="histidine kinase"
                     /note="from Clostridium thermocellum Clo1313_1973"
                     /protein_id="AGO62061.1"
                     /translation="MKKRRTIGFLVNDIDVSYSNLVYLMTKKAAEKYDCNLIVYEGRSF
                     NNPSMADSQHQISYSFVDNTRLDGIIITSATIACFISDEEFVEFCKRYEGIPLVSLGIV
                     VPHATSILVDNKPGMKSQVEHLIKDHGFKKIVYISGPPTNTDSVERLEAYCEALRENDI
                     EVDDGLIFYGDFMAQSGYDIIKEIIKNGIEYDAVVCANDEMAFGAIKGIRDLKDAYNLD
                     LTQKGVICGFDDSITSRLVKPSLTTVRQPIEEMCFYAVEAILKKIEGEKVEDVVKLPSV
                     LVKRESCGCKNAAEVDFVSDSSLRLVPGQRIHENLQTYSLDELFDCITRTLKLCSIRSC
                     FIFKYCGGTLLYEVDSIFRDDFRLPEDSELIYAYYNDERITIEDSIKYIKTRCIIPDRF
                     FNEDRRFTYLVNPLFFYDEHFGYVCFEVANDDVLNFEPIRGQISNTLNGALMLMERAKM
                     EESLRETERLASLGQLIGGISHNLMTPIMSISGACFGLEDLVHEYMASIEDPSVTVQDH
                     KEIARDMQEWINKLKEYNSYMSNVIATVKSQAVQLNSDAINEFTIEELVDRVKFIKNNN
                     PQIRKAVLNCKIDIDGKTAIRGDISNLAQIIENLLINAVQSYEGMDNASNIVDLHILKE
                     ENMVKCIVRDYGKGIPNEIRDRLFRRMVTTKGKNGTGLSLLLSYSTIKGKFGGELWVES
                     KENEGSAFYVTIPLTDRE"
     terminator      2189..2436
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2684..3272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3359..3937)
                     /codon_start=1
                     /product="tdk"
                     /label="tdk"
                     /protein_id="AGO62062.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     regulatory      3938..4558
                     /label="cbp promoter"
                     /note="cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(4637..5638)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             complement(6199..7056)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(7155..7955)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(7956..8176)
                     /label="URA3 promoter"
     misc_feature    8204..8707
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_recomb     8868..9809
     misc_recomb     9810..10673
     regulatory      10691..11338
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            10691..11338
                     /gene="gapD"
                     /label="gapD"
     CDS             11339..11986
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             12007..12552
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AGO62065.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            12007..12552
                     /gene="hpt"
                     /label="hpt"
ORIGIN
        1 atgaaaaagc gcaggacgat tggttttttg gttaacgaca tagatgtaag ctattccaat
       61 ttggtttacc ttatgaccaa aaaggccgca gaaaaatatg actgcaacct tattgtttat
      121 gaaggcagat ctttcaacaa cccttccatg gcagacagcc agcatcaaat atcctacagc
      181 tttgttgaca atacccgttt ggacggaata ataattacaa gtgccacgat tgcgtgcttt
      241 atttccgatg aggaatttgt agaattttgc aaacgttatg aaggaatacc tttggtatcg
      301 ctgggaattg ttgttcccca tgccaccagt attcttgtgg acaacaaacc gggaatgaaa
      361 agccaggtgg aacatcttat aaaagaccat ggttttaaaa aaattgttta tatatccggt
      421 cctccgacaa atacggattc ggtcgaacgt cttgaagcat actgtgaagc gctgagggaa
      481 aatgatattg aggtagatga cggtctgata ttttacgggg attttatggc acaatccgga
      541 tatgacatta taaaagagat aataaagaac ggcattgagt atgatgcggt tgtttgtgca
      601 aatgatgaga tggcttttgg agcgataaaa ggtataaggg acttaaaaga cgcttataat
      661 ctcgacttaa cccaaaaagg tgtgatatgc ggatttgatg attccataac gtcgcgactg
      721 gtcaaaccct ctttgactac ggtaagacag cctattgaag aaatgtgttt ttatgcggtt
      781 gaggcaattc ttaaaaaaat agaaggagaa aaagttgaag atgtggtgaa acttccttct
      841 gtcctggtta aaagggaatc ctgcggctgt aaaaacgcgg ccgaagtgga ttttgtgagt
      901 gacagcagtc taagacttgt tcccggtcag aggatacatg agaatttgca gacatattcc
      961 ctggatgaat tatttgactg tattaccaga acattaaaac tgtgttccat cagaagctgt
     1021 tttattttca aatattgcgg cggaactttg ctgtatgagg ttgattctat tttcagagac
     1081 gattttagac tgcctgagga ttccgaattg atatatgctt actataatga cgagagaata
     1141 accatagaag acagtattaa atatataaaa acaagatgca ttataccgga ccggttcttt
     1201 aacgaagaca ggcggtttac atatttggtt aatccattgt ttttttatga cgaacacttt
     1261 ggttatgtgt gttttgaagt agcaaatgat gatgtactga actttgaacc tatcaggggt
     1321 cagataagca acacgttaaa cggtgcgctg atgctcatgg aaagagccaa gatggaagaa
     1381 agcctcaggg agacggaacg tcttgcttcg ctggggcagc ttatcggagg aatttcccac
     1441 aatcttatga ctcctataat gtccatatcc ggagcatgct ttggattgga agaccttgtg
     1501 catgaatata tggcatctat agaagaccct tccgttacgg tgcaggacca caaggaaata
     1561 gccagggaca tgcaggagtg gataaacaaa ctcaaagaat acaattcgta catgtcgaat
     1621 gttattgcaa cggtcaaatc acaagcggtc cagcttaatt ccgatgcgat taacgagttt
     1681 acaatcgaag agcttgtaga cagagttaaa tttataaaaa acaataaccc ccagatcaga
     1741 aaagccgttt taaactgcaa aattgatatt gacggtaaaa cggcaattcg aggggatata
     1801 tccaatctgg cacagataat tgaaaatctc ttgattaatg cagtgcagtc ttatgaagga
     1861 atggataatg ccagtaacat tgttgatctc catatattga aagaagaaaa tatggtcaaa
     1921 tgtattgtca gagattatgg gaaaggcata cccaatgaaa taagagacag gctgtttagg
     1981 cgaatggtaa ccacaaaagg taaaaatgga acgggactga gcctgttatt gtcctattcc
     2041 acaataaagg gcaagttcgg aggggaattg tgggtggagt caaaggaaaa cgaaggttct
     2101 gcgttttatg taacgatacc tttgaccgac agggaatgat aattcggcgc gcctcagcgt
     2161 ttaaacccgc tgatcctaga gggccgcatc atgtaattag ttatgtcacg cttacattca
     2221 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     2281 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     2341 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     2401 gagaaggttt tgggacgctc gaaggcttta atttgcaagc tgcggccctg cattaatgaa
     2461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2641 agcaaaagcc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3121 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaaa aaatccgctt
     3361 aagtccgcgg gacttcatgg cactttctac accttgcttc ataagactcc tttgctccaa
     3421 ccattataat gggatcgtcg taattggcag gcttcccatt tataagcctt tgcgtgcgag
     3481 tagcaggatt gccacacttc atgcatatag cagtaagctt atcgacaaat tcagctatgg
     3541 ccatcaattc tggagtcgga ccaaatggtt cacctctaaa gtccatgtca agccctgcac
     3601 atatgactct tttcccgcta tcggcaatct cttttacgat gtcgactatt tcagaatcaa
     3661 aaaattgaac ttcatctata gcaaatacat ccgtatcttc ttcagcataa gccaatatgt
     3721 cagaagcctt tactatggca atggcgtgca tgttgtcgcc gttatgagat acgactttgt
     3781 ctatggaata cctatcgtct atagccggtt tgaaaacctg aactttttgc ctggcaatct
     3841 tagctctctt tatccttctt ataagttcct cactttttcc actgaacatg ggaccagtta
     3901 caacttctat gtacccgtgg tctttaggcc catacatctt tcgtcctcct taaaattttc
     3961 gtttatttgt tttttattta gtgtgcctaa aagcaccatc ttatacattc gtaccttata
     4021 tctcttatct tgtatctctt acctcatatc tcttattatt aactaccgaa gtatattttc
     4081 ataacggaat cttatgactt atttttctga ctgccgtttt accatacggt taatttatac
     4141 ggaaacagct aatctaacct tgctgaaggc atgtttacca aaatattata tctactttac
     4201 catattaatt caagtggtca ttttgatatt ttacgccaaa atttttagcg ttcctttact
     4261 tgataattta tccaaaaacc ttcattcgtc aaaacaatta tgatataatt aatttatgat
     4321 atgattaatt aatatttacg gataactaat catttcaatg caatagatca acatgcaaat
     4381 caatatccac cttactccga acattttgca ccaacgaatg cccagcacta tatcccggca
     4441 agcaatataa cgtctataat tttttatgat aacgataaaa ttaaatttta tgaaaggggt
     4501 aaaagcatga gaaaaatagc tgtattgtta attactttga cgttcttagt cacgactcga
     4561 atttaggagg cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat
     4621 cccgtttgtt gaactactct ttaataaaat aatttttccg ttcccaattc cacattgcaa
     4681 taatagaaaa tccatcttca tcggcttttt cgtcatcatc tgtatgaatc aaatcgcctt
     4741 cttctgtgtc atcaaggttt aattttttat gtatttcttt taacaaacca ccataggaga
     4801 ttaacctttt acggtgtaaa ccttcctcca aatcagacaa acgtttcaaa ttcttttctt
     4861 catcatcggt cataaaatcc gtatccttta caggatattt tgcagtttcg tcaattgccg
     4921 attgtatatc cgatttatat ttatttttcg gtcgaatcat ttgaactttt acatttggat
     4981 catagtctaa tttcattgcc tttttccaaa attgaatcca ttgtttttga ttcacgtagt
     5041 tttctgtatt cttaaaataa gttggttcca cacataccaa tacatgcatg tgctgattat
     5101 aagaattatc tttattattt attgtcactt ccgttgcacg cataaaacca acaagatttt
     5161 tattaatttt tttatattgc atcattcggc gaaatccttg agccatatct gacaaactct
     5221 tatttaattc ttcgccatca taaacatttt taactgttaa tgtgagaaac aaccaacgaa
     5281 ctgttggctt ttgtttaata acttcagcaa caaccttttg tgactgaatg ccatgtttca
     5341 ttgctctcct ccagttgcac attggacaaa gcctggattt acaaaaccac actcgataca
     5401 actttctttc gcctgtttca cgattttgtt tatactctaa tatttcagca caatctttta
     5461 ctctttcagc ctttttaaat tcaagaatat gcagaagttc aaagtaatca acattagcga
     5521 ttttcttttc tctccatggt ctcacttttc cactttttgt cttgtccact aaaacccttg
     5581 atttttcatc tgaataaatg ctactattag gacacataat attaaaagaa acccccatct
     5641 atttagttat ttgtttggtc acttataact ttaacagatg gggtttttct gtgcaaccaa
     5701 ttttaagggt tttcaatact ttaaaacaca tacataccaa cacttcaacg cacctttcag
     5761 caactaaaat aaaaatgacg ttatttctat atgtatcaag aatagaaaga actcgttttt
     5821 cgctacgctc aaaacgcaaa aaaagcactc attcgagtgc tttttcttat cgctccaaat
     5881 catgcgattt tttcctcttt gcttttcttt gctcacgaag ttctcgatca cgctgcaaaa
     5941 catcttgaag cgaaaaagta ttcttctttt cttccgatcg ctcatgctga cgcacgaaaa
     6001 gccctctagg cgcataggaa caactcctaa atgcatgtga ggggttttct cgtccatgtg
     6061 aacagtcgca tacgcaatat tttgtttccc atagcggccg cgattatcaa aaaggatctt
     6121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggagcg
     6301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6481 taatagtttg cgcaacgttg ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt
     6541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aatagtgtat cacatagcag
     6841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatgggtaat
     7081 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     7141 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     7201 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     7261 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     7321 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     7381 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     7441 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     7501 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     7561 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     7621 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     7681 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     7741 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     7801 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     7861 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     7921 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt
     7981 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     8041 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     8101 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     8161 gatgaattga attgaaaagc tagcttatcg atgggtcctt ttcatcacgt gctataaaaa
     8221 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     8281 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     8341 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     8401 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     8461 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     8521 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     8581 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
     8641 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
     8701 agcgatccgt ccgccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     8761 ggctagggcg gtgggaagtg taggggtcac gctgggcgta accaccacac ccgccgcgct
     8821 taatggggcg ctacagggcg cgtggggatg atccactagt aagcttgggc ggcaaatctt
     8881 cttgaggcca tgaacaaagt gctttcggaa aagataagaa aaaattgtat ctggattgac
     8941 tgggacccta acaaggaaag ccttacaggt gcattattaa aacaggtggt gggttaaagg
     9001 ttgaaggtgt ggacgttgtc ggctcgaatt ttgttatcaa ttgcggctta tgtgttgtcc
     9061 tatattcttt ttgtctgttc atccatttat tgggaccttg tatttttatt taaaacacag
     9121 agcagagttt ttataataat aaatattgta gcttttatca taatcgggct gtgttacaat
     9181 aagtttgaaa aagcagtcgg tgtgatatgc atgctctttg cggctccttc tgtgttggtt
     9241 cattcaaaaa tgctcaaaag tgagccggca gagtcagtat atacgagata ttttccactc
     9301 cacttgaccg cccttttatt tttgataatt actgttcttt taatggcggc aataagactt
     9361 caaaaactcg acagacagta tgatgaaatg atatccggcg gtgcgtcgga ggccgatgta
     9421 aagttaataa ttcttaacaa tatcaaagtg tattccgttt ttcttgctgt aattctattc
     9481 agcggatttg ttcttgttgc tttggccttt gcaatgtcta aaatcaaagc aacgcggttg
     9541 actgtaatta taacggtaac aaccggcgtt gtgctggttt tagggtgtgt actgtatttg
     9601 tacagaaaat ggactaaaaa atagtaattt aataacaagt ttttgttata tatatttttt
     9661 taattttatg gtataacctt aaaaactctg tttttatttt ccgatataca taatatattc
     9721 cgatatgttc ttgtgagtct tgtgaaatca ttccatatca ctgaaataat tcatatacat
     9781 aatataatat attttaagga gaagccttag ggaagggctc cttctttcag aataaacggg
     9841 aaaatgaaaa cgtttactga caatatactg tctccggaag atacaatgag ctatgccaaa
     9901 gagtgtttgg acaaggaaaa gcttgagcat cttatggctg tgggagaagt tgatttttca
     9961 atgacggttc ccggaatttc caggttcagg gtgaatgtgt tttttcaaag gggctctgtg
    10021 gcaatagtat tcagaatact tgcttccaca ataccgactc tggattcttt gaattttcct
    10081 gaagtgatta aggacatttg caaaataaaa gaagggctca tacttgtaac cggccccacg
    10141 ggaagcggaa aatccactac catggcttca attatcaatg aaataaactc taccagggaa
    10201 gggcatatat tgactcttga agaccctatc gaatatattt tcaagcatga caaatgtgtc
    10261 gtaaaccaga gggaaatatc ccaggacagc aagtcctatg ccagtgcttt aaaagctgcc
    10321 ttaagagaag accccgatgt gatattcata ggggagatga gggacaatga gtctataagt
    10381 attgcggtta cggcggcaga gacggggcac ctggttcttt caaccctgca tactctgggt
    10441 gcggctaaaa ccatagacag gcttattgac gtttttccac cccaccagca acagcagata
    10501 agggtccagg tatccagtgc gctaaaggcg gttatatccc agagattgat tcccgatatt
    10561 gataacaaag gaagagttgc ggctcttgaa ataatgattg ttactccggc aatcagcaat
    10621 ctcataaggg aaggcagagt ggcaggaatt acttcaatga ttcagacggg tgcgatcctc
    10681 gcgaggccgg ccagtattct gacatgggtg tatcaataac ccatgcgttt ccgtattgta
    10741 tcggaatggt ttcggacagg gcggtgggaa tagacatgga aaagattttt ttgcccgagg
    10801 atgcattgat aaagtatttc ttttccgaaa gagaggaaaa gattctaaag agttttggaa
    10861 atactgatga atattgtgtg cagagtacaa ttctatggac aagaaaagag gctttgtcaa
    10921 aactttttcg tctgggaatg aggatggatt ttaaaaagct ggatactttg gaggacgagg
    10981 tggtttttca ggaaacaaac agggcgcgtc tgttttcttt tatatgcaat aattactgta
    11041 tctctctggc attgccaggt tttaataaag attaaaatta ttgactagaa ataaaaaaat
    11101 tgtccataat attaatggac aaaaaaacaa agaattacat caaaggaaga taaaaatact
    11161 ttgttaaaaa attaattatt ttttatctaa actattgaaa atgaaaataa aataatataa
    11221 aatgaatcat agtgcaagag atacttgcca gaggatgaat attttactgc attcatgctt
    11281 tatggcagct aatagaggca ttaaattaaa ttttaattta caataggagg cgatattaat
    11341 gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt
    11401 gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa
    11461 cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat
    11521 aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag gttattggga
    11581 taagttagag ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg
    11641 gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa
    11701 atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct
    11761 ttctattatt ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa
    11821 ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt
    11881 accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat
    11941 gaactctatt caggaattgt cagataggcc taatgactgg cttttataat aaaggaggtc
    12001 gacgtcatgg aaaatttatc aaaagacatc gatgaaattt tgatcacaga agaagaactt
    12061 aaggaaaaga taaaagagct tgggaggcaa atcacaaaag actacaaagg gaaaaatttg
    12121 atgttggtag gagttttaaa aggtgcttta atgtttatgg ctgatttgtc aagacacata
    12181 gatttgcctt tatcacttga ttttatggct gtttccagct atggaagctc aactcattca
    12241 tcaggaatag taaagataat caaagatctt gatataagca tagaaggcaa agatgttctg
    12301 attgtggaag acataattga cagcggtttg actttgtctt acttaaggga aactttactt
    12361 ggaaggaagc caaaaagcct gaaaatatgc acaatattag acaaaccgga gagaagagaa
    12421 gcatctgtaa aagtcgatta tgtaggattt aagatacctg ataagtttgt cgtgggttat
    12481 ggattggact ttgatgaaaa gtacaggaac cttcctttta taggcgtttt gaaacctgaa
    12541 atgtacagct aagcccgggc ctcgagaaaa caaaaggctc agtcggaaga ctgggccttt
    12601 tgttttggta ccg
//
