LOCUS       V007698                 7803 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007698
VERSION     V007698
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Derouazi M, Toussaint B, Quenee L, Epaulard O, Guillaume M, Marlu R,
            Polack B.
  TITLE     High-yield production of secreted active proteins by the Pseudomonas
            aeruginosa type III secretion system
  JOURNAL   Appl. Environ. Microbiol. 74 (11), 3601-3604 (2008)
   PUBMED   18390679
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Toussaint B, Le Gouellec A, Polack B.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2012) TIMC-TheREx Laboratory, Universite Joseph
            Fourier, Doamine de la merci, La Tronche 38700, France
REFERENCE   3  (bases 1 to 7803)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7803)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "11";
            pages: "3601-3604"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-FEB-2012) TIMC-TheREx Laboratory, Universite Joseph Fourier,
            Doamine de la merci, La Tronche 38700, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..162
                     /codon_start=1
                     /gene="exoS"
                     /product="ExoS54"
                     /label="exoS"
                     /note="secretion tag; first 54 amino acid of ExoS"
                     /protein_id="AFD22622.1"
                     /translation="MHIQSLQQSPSFAVELHQAASGRLGQIEARQVATPSEAQQLAQRQ
                     DAPKGEGLL"
     gene            1..162
                     /gene="exoS"
                     /label="exoS"
     primer_bind     complement(211..227)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(391..1221)
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      1235..1586
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        1913..2017
                     /label="AmpR promoter"
     CDS             2018..2875
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3049..3637
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3925..3946
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3961..3991
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3999..4015
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4023..4039
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4070..5149)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5150..5227)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     terminator      complement(5596..5623)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5716..5802)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     6203..6219
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(6240..7073)
                     /gene="exsA"
                     /label="HTH-type transcriptional regulator ExsA"
                     /note="HTH-type transcriptional regulator ExsA from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P26993"
     protein_bind    complement(7108..7124)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(7266..7616)
                     /codon_start=1
                     /product="SpcS"
                     /label="SpcS"
                     /protein_id="AFD22626.1"
                     /translation="MNPLYRAAIHQLFLALDLPTPNDEESVLSLQVGPHLCHLAEHPTD
                     HLLMFTRLEGQGDATASEQNLFSQDPCKPILGRDPESGERLLWNRQPLQLLDRAQIHHQ
                     LEQLVAAAEELR"
ORIGIN
        1 atgcatattc aatcgcttca gcagagtccg tctttcgccg tcgaattgca ccaggccgcc
       61 agtgggcgtt tgggacagat tgaggcccgc caggtcgcca cccccagtga agcgcagcag
      121 ttggcccagc gccaggacgc gccgaagggt gaggggctgc tcggatccac cggtgctagc
      181 atgtacagag atctagcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
      241 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      301 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaaagg
      361 caggccgggc cgtggtggcc acggcctcta ggccagatcc agcggcatct gggttagtcg
      421 agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt ttcgcgatct cagcatctga
      481 aatcttcccg gccttgcgct tcgctggggc cttacccacc gccttggcgg gcttcttcgg
      541 tccaaaactg aacaacagat gtgtgacctt gcgcccggtc tttcgctgcg cccactccac
      601 ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga tcaagcactc gcaacttgaa
      661 gtccttgatc gagggatacc ggccttccag ttgaaaccac tttcgcagct ggtcaatttc
      721 tatttcgcgc tggccgatgc tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg
      781 ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc
      841 cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct acacggccct caccctcccg
      901 gtagatgatt gtttgcaccc agccggtaat catcacactc ggtcttttcc ccttgccatt
      961 gggctcttgg gttaaccgga cttcccgccg tttcaggcgc agggccgctt ctttgagctg
     1021 gttgtaggaa gattcgatag ggacacccgc catcgtcgct atgtcctccg ccgtcactga
     1081 atacatcact tcatcggtga caggctcgct cctcttcacc tggctaatac aggccagaac
     1141 gatccgctgt tcctgaacac tgaggcgata cgcggcctcg accagggcat tgcttttgta
     1201 aaccattggg ggtgaggcca cgttcgacat tccttgtgta taaggggaca ctgtatctgc
     1261 gtcccacaat acaacaaatc cgtcccttta caacaacaaa tccgtccctt cttaacaaca
     1321 aatccgtccc ttaatggcaa caaatccgtc cctttttaaa ctctacaggc cacggattac
     1381 gtggcctgta gacgtcctaa aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca
     1441 aaaaacgcac cactacgtgg ccccgttggg gccgcatttg tgcccctgaa ggggcggggg
     1501 aggcgtctgg gcaatccccg ttttaccagt cccctatcgc cgcctgagag ggcgcaggaa
     1561 gcgagtaatc agggtatcga ggcggattca cccttggcgt ccaaccagcg gcaccagcgg
     1621 cgcctgagag gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1681 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1741 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1801 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1861 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1981 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2041 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2101 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2161 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2221 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2281 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2341 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2401 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2461 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2521 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2581 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2641 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2701 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2761 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2821 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2881 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2941 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3001 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3181 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3661 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3721 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3781 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3841 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3901 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     3961 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     4021 cacaggaaac agctatgacc atgattacga attcgagctc ggtacctcac tgcccgcttt
     4081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4141 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     4201 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     4261 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     4321 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     4381 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     4441 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     4501 cccgttccgc tatcggctga atttgattgc cgagtgagaa attatgccag ccagccagac
     4561 gcagacgcgc cgagacagaa cttaatgggc ctgctaacag cgcgatttgc tggtgaccca
     4621 atgcgaccag atgctcacga cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     4681 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     4741 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     4801 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     4861 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     4921 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     4981 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     5041 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     5101 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     5161 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga
     5221 tggtgtcggg tacagaacct cacgtctaag aaaccattat tatcatgaca ttaacctata
     5281 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     5341 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5401 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     5461 cggcatcaga gcagattgta ctgagagtgc accataaatt ccctgctttc ctgatgcaaa
     5521 aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg acaggaagag
     5581 tttgtagaaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
     5641 gcagtttatg gcgggcgtcc tgcccgccac ccctccgggc cgttgcttcg caacgttcaa
     5701 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     5761 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5821 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     5881 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac
     5941 cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac
     6001 agggaattta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     6061 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     6121 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     6181 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctttc aaaacgtcag
     6241 ttatttttag cccggcattc gtccttcccc tgccgcgagc ggctcggtgt gcagccgaaa
     6301 cggcggcgat agctctgggt gaaataggac tgactggaaa agcccgcctc catggcgatg
     6361 tcgacgatgc tcatgtcgct gttgagcagc aactgatggg catagaggat tctccgctcg
     6421 ctgatccagg cgcgcggcga aaccccgtag acactgccga acagctcctt gaaggtggtc
     6481 agccccatgc cgaactcgcg ggagaagtcg gacagcttcc actcgttgag gtagtgcttc
     6541 tccatgaata gctgcagacg ctcgacatgc cggttgctca gttgccgcag gaccgacatc
     6601 agcagcggcc cctgcggact gaacgcgaag agcatcagca actcctcgat cttcaggcag
     6661 gcgagcatcg gcggatgctc atgcacaagc aattccttca accccttgac gcaaccggcc
     6721 agcagaggcg tggcagcgaa cgcgatgatg cccggcacgg gctcgtcgca atgctcgact
     6781 tcactcaaca gcgcgccgaa gcgctggacg aagccttgta gaaactgggc agataatgga
     6841 atccagagta ttcggctgtc ctttcccttg gtacttacga catagcttcc gcggcggacg
     6901 aaaagcaact cgccaggcgc caggcaaaaa gtggaatcga tgtcctggac ggtcagttcg
     6961 ccctcgagca gaacatatac gccctcttcc ttgtttaccc tgtattcgaa agttggaatg
     7021 ttccaatgac aagacgttat ctgctttcgg ccgagagatt tggctccttg catattataa
     7081 gaattcatcg ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg
     7141 aggcgatgaa ttgtcaatct agagggcagg tgctccggct ggctcggcat ggtcaatgga
     7201 gttaagcccc gggggcttcg aggaaagcag aacctgcggc atcggctcct ttcgcccgac
     7261 tgggctcagc gtagctcttc ggcggcggcg accagttgtt cgagttgatg gtggatctgg
     7321 gccctgtcca gcaactgcaa gggctggcgg ttccagagca gccgttcgcc gctttcgggg
     7381 tcgcggccca ggatcggctt gcaagggtcc tggctgaaca ggttctgctc gctggccgta
     7441 gcgtctcctt gtccttccag gcgggtgaac atcaacaggt gatcggtcgg atgttctgcc
     7501 agatggcaga gatgcgggcc cacctgcagg ctgagtacgc tctcctcgtc attgggcgtc
     7561 gggagatcga gagcgagaaa aagctggtgg atggcggcgc ggtagagtgg attcatggcg
     7621 tgttccgagt cactggaggc ggccattaga gcagtgccag cccggagaga ctgttaatcg
     7681 tggttctctt tttttaggtt ttgccgctgc cgattccagt gaaaaaacgg cggccaatcc
     7741 tgataggcga tggggtttcc cgttcctaga ctggcggaga aacatcagga gaaggcaacc
     7801 atc
//
