LOCUS       62056_8680        7861 bp DNA     circular SYN 02-MAR-2020
DEFINITION  Cloning vector pEA206, complete sequence.
ACCESSION   MN161744
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Liu C, Lin L, Wang J.
  TITLE     Absolute quantification of real-time PCR data with stage signal 
            difference analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Liu C, Lin L, Wang J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) State Key Laboratory of Electroanalytical 
            Chemistry, Changchun Institute of Applied Chemistry, Renmin Street, 
            Changchun, Jilin 130000, China
REFERENCE   3  (bases 1 to 7861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2019) State Key Laboratory of Electroanalytical Chemistry, 
            Changchun Institute of Applied Chemistry, Renmin Street, Changchun, 
            Jilin 130000, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        847..1551
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1569..1589
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1587..2192
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             2214..4079
                     /codon_start=1
                     /transl_table=11
                     /product="PhyB-N"
                     /label=PhyB-N
                     /protein_id="QID75589.1"
                     /translation="MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSL
                     RPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAY
                     LSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDV
                     RSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTE
                     DPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHED
                     EHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRL
                     TQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGL
                     VVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRD
                     SPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLS
                     TDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDG
                     QRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKES"
     terminator      4117..4304
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             4337..4360
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             complement(4431..5102)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(5103..5204)
                     /label=TRP1 promoter
     terminator      5276..5323
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5420..5875
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5901..6005
                     /label=AmpR promoter
     CDS             6006..6863
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7037..7625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agataaaaca agaagagggt tgactacatc acgatgaggg ggatcgaaga aatgatggta
      121 aatgaaatag gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa
      181 ataaagtgaa aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta
      241 cgcaattgca caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata
      301 acgctgggcg tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac
      361 cctgcgctaa ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg
      421 acgaccacga caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa
      481 catgagtata ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga
      541 acaatagagc gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag
      601 aggaaacagc aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat
      661 ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata
      721 tggaagggaa ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag
      781 agaagggggg taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt
      841 tcggatatcc ttttgttgtt tccgggtgta caatatggac ttcctctttt ctggcaacca
      901 aacccataca tcgggattcc tataatacct tcgttggtct ccctaacatg taggtggcgg
      961 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
     1021 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
     1081 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
     1141 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
     1201 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
     1261 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
     1321 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
     1381 tccttccagt tacttgaatt tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa
     1441 atagacctgc aattattaat cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct
     1501 tgtttctttt tctgcacaat atttcaagct ataccaagca tacaatcaac tatctcatat
     1561 acataatgcc taaaaagaag cgtaaagtga aagcgttaac ggccaggcaa caagaggtgt
     1621 ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg
     1681 cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac
     1741 gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag
     1801 aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccactt ctggcgcaac
     1861 agcatattga aggtcattat caggtcgatc cttccttatt caagccgaat gctgatttcc
     1921 tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggt gacttgctgg
     1981 cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg
     2041 aagttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg ccagaaaata
     2101 gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt gaagggctgg
     2161 cggttggggt tattcgcaac ggcgactggc tggaattccc ggggatccaa actatggttt
     2221 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     2281 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     2341 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     2401 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     2461 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     2521 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     2581 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     2641 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     2701 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     2761 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     2821 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     2881 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     2941 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     3001 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     3061 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     3121 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     3181 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     3241 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     3301 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     3361 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     3421 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     3481 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     3541 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     3601 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     3661 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     3721 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     3781 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     3841 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     3901 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     3961 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     4021 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatcttgac
     4081 atatgtcgac gagctctgca gccaagctaa ttccgggcga atttcttatg atttatgatt
     4141 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     4201 gttttaaaac gaaaattctt gttcttgagt aactctttcc tgtaggtcag gttgctttct
     4261 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggctagca tgactggtgg
     4321 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagatctgc
     4381 aggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta tttcttagca
     4441 tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg
     4501 cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc
     4561 agtccaccag ctaacataaa atgtaagctt tcggggctct cttgccttcc aacccagtca
     4621 gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga
     4681 ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat
     4741 acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct
     4801 ccatgcagtt ggacgatatc aatgccgtaa tcattgacca gagccaaaac atcctcctta
     4861 ggttgattac gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt tttatatgct
     4921 tttacaagac ttgaaatttt ccttgcaata accgggtcaa ttgttctctt tctattgggc
     4981 acacatataa tacccagcaa gtcagcatcg gaatctagag cacattctgc ggcctctgtg
     5041 ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt aataacagac
     5101 atactccaag ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa tagtcaccaa
     5161 tgccctccct cttggccctc tccttttctt ttttcgaccg aattcgaagc ttgatccggc
     5221 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     5281 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     5341 atccggatct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5401 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5461 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5521 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5581 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5641 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5701 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5761 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5821 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7801 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7861 g
//
