LOCUS       Exported                4933 bp DNA     circular SYN 21-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        117..148
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      204..213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             210..1862
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1903..2024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2443..3031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3202..4062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4063..4167)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4194..4649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    4780..4828
                     /note="synthetic polyadenylation signal"
     misc_feature    4842..4933
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 atcgataggt accgagctct tacgcgtgct agccttggcg ggagatagaa cttggcggga
       61 gataggaact tggcgggaga taggaacttg gcgggagata ggaaagatct agactctaga
      121 gggtatataa tggaagctcg aattccagct tggcattccg gtactgttgg taaaaagctt
      181 ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc
      241 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
      301 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
      361 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
      421 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
      481 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
      541 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
      601 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
      661 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
      721 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
      781 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
      841 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
      901 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
      961 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     1021 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
     1081 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     1141 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     1201 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     1261 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     1321 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     1381 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     1441 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     1501 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1561 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1621 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1681 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1741 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1801 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
     1861 aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt
     1921 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1981 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     2041 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     2101 tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac
     2161 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2221 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     2281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     4201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4621 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
     4681 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
     4741 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
     4801 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4861 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4921 agaacatttc tct
//
