LOCUS       40924_16265        7306 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant expression vector pDuExAn1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Fujikawa Y, Kato N.
  TITLE     Split luciferase complementation assay to study protein-protein 
            interactions in Arabidopsis protoplasts
  JOURNAL   Plant J. 52 (1), 185-195 (2007)
  PUBMED    17662028
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Kato N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2007) Dapartment of Biological Sciences, Louisiana
            State University, 226 Life Sciences Building, Baton Rouge, LA 
            70803-1715, USA
REFERENCE   3  (bases 1 to 7306)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "52"; issue: "1"; pages: "185-195"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-APR-2007) Dapartment of Biological Sciences, Louisiana State 
            University, 226 Life Sciences Building, Baton Rouge, LA 70803-1715, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(89..122)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        642..987
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1016..1071
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1105..1821
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    1840..1964
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2111..2213
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2214..2870
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3215..3517
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3561..3685)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3709..3961
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3970..3986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4380..4398)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4416..4432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4440..4456)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4464..4494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4509..4530)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4818..5406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5580..6437)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6438..6542)
                     /label=AmpR promoter
     rep_origin      complement(6620..7075)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7216..7232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7239..7257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7288..7297
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctgtaatac gactcactat agggcgctag ctgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggccgcat aacttcgtat agcatacatt atacgaagtt
      121 atcagtcgac aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag
      181 aatgctaacc cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac
      241 ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa
      301 agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt
      361 actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt
      421 ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata
      481 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga
      541 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc
      601 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
      661 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
      721 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
      781 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
      841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
      901 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
      961 ggaagttcat ttcatttgga gagaacacgg gggactctag ctagatattt ttacaacaat
     1021 taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta cagtcgatct
     1081 gatcaagaga caggatcctc tagaatggtg agcaagggcg aggagctgtt caccggggtg
     1141 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1201 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1261 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     1321 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1381 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1441 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1501 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1561 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1621 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1681 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     1741 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1801 ggcatggacg agctgtacaa gagatctgag ctcctcgaga caagtttgta caaaaaagct
     1861 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
     1921 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gctaagttgg
     1981 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag
     2041 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     2101 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     2161 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     2221 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     2281 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     2341 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     2401 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     2461 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     2521 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     2581 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     2641 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     2701 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     2761 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     2821 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
     2881 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     2941 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     3001 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3061 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3121 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3181 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     3241 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     3301 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     3361 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     3421 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     3481 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     3541 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     3601 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     3661 gttcagcttt cttgtacaaa gtggtccgag ctcagctcga atttccccga tcgttcaaac
     3721 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     3781 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3841 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3901 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3961 cgggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct ttcgctttct
     4141 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4201 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4261 atggttcacg tagtgcagct cgatatcaaa ttacgccccg ccctgaatca ctagtgcggc
     4321 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt
     4381 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4441 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6541 cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag
     6601 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     6661 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     6721 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     6781 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     6841 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     6901 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     6961 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7021 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
     7081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7141 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7201 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
     7261 aattgggccc gacgtcgcat gctcccggcc gccatggccg cgggat
//
