LOCUS       40924_15675        6402 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pDsRed-N-GW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6402)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6402)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(259..714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        741..845
                     /label=AmpR promoter
     promoter        847..1204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1239..2030
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2265..2312
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2641..3229
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        3404..3707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3708..3911
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3956..4630
                     /codon_start=1
                     /label=DsRed-Monomer
                     /note="monomeric derivative of DsRed fluorescent protein 
                     (Strongin et al., 2007)"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     protein_bind    4694..4818
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4843..4873
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4927..5583
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5928..6230
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6274..6398)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
       61 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      121 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      241 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
      481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
      541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
      721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      841 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
      901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1021 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1081 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1141 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1201 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     1261 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1321 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1381 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     1441 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1501 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1561 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1621 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1681 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1741 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     1801 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1861 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1921 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1981 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     2041 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     2101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     2161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     2221 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     2281 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     2341 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     2401 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     2461 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     2521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2761 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3301 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     3361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3721 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3841 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3901 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgga
     3961 caacaccgag gacgtcatca aggagttcat gcagttcaag gtgcgcatgg agggctccgt
     4021 gaacggccac tacttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca
     4081 gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     4141 cccccagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     4201 catgaagctg tccttccccg agggcttcac ctgggagcgc tccatgaact tcgaggacgg
     4261 cggcgtggtg gaggtgcagc aggactcctc cctgcaggac ggcaccttca tctacaaggt
     4321 gaagttcaag ggcgtgaact tccccgccga cggccccgta atgcagaaga agactgccgg
     4381 ctgggagccc tccaccgaga agctgtaccc ccaggacggc gtgctgaagg gcgagatctc
     4441 ccacgccctg aagctgaagg acggcggcca ctacacctgc gacttcaaga ccgtgtacaa
     4501 ggccaagaag cccgtgcagc tgcccggcaa ccactacgtg gactccaagc tggacatcac
     4561 caaccacaac gaggactaca ccgtggtgga gcagtacgag cacgccgagg cccgccactc
     4621 cggctcccag tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
     4681 cgcgggccca tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     4741 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     4801 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     4861 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
     4921 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     4981 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     5041 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     5101 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     5161 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     5221 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     5281 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     5341 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     5401 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     5461 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     5521 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     5581 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     5641 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     5701 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     5761 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     5821 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     5881 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     5941 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     6001 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     6061 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     6121 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     6181 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     6241 gctccgttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     6301 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     6361 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg at
//
