LOCUS       40924_15665        5748 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1287
                     /label=DsRed-Monomer
                     /note="monomeric derivative of DsRed fluorescent protein 
                     (Strongin et al., 2007)"
     misc_feature    1288..1353
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1477..1598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1605..2060)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2087..2191
                     /label=AmpR promoter
     promoter        2193..2550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2558..3580
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    3700..3781
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        3895..3999
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             4000..4857
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5046..5634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg
      661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag
      721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc
      841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc
      901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc
      961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg
     1021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg
     1081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac
     1141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac
     1201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac
     1261 gccgaggccc gccactccgg ctcccagtcc ggactcagat ctcgagctca agcttcgaat
     1321 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     1381 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1441 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1561 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     1621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1801 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1861 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1921 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1981 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     2041 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2161 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     2221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2521 ggcttttttg gaggcctagg cttttgcaaa gatcgatatg aaaaagcctg aactcaccgc
     2581 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     2641 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     2701 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     2761 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     2821 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     2881 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     2941 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     3001 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     3061 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     3121 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     3181 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     3241 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     3301 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     3361 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     3421 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     3481 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     3541 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagttcta gaggatcata
     3601 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3661 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3721 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3781 cctcgcgatc cacaaccatg gcgaaagggc ctcgtgatac gcctattttt ataggttaat
     3841 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3901 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3961 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4021 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4081 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4141 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4201 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4261 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4321 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4381 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4441 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4501 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4561 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4621 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4681 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4741 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4801 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4861 ggcgcgccag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5161 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5701 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
