LOCUS       62056_8475        7372 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        655..673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1714..1965
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2809..2838
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2842..2988
                     /codon_start=1
                     /label=PDGFR-beta TM domain
                     /note="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    3016..3240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3372..3960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(4289..4336)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4571..5362)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5405..5611)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     enhancer        complement(5689..5880)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     CDS             complement(5943..6800)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6801..6905)
                     /label=AmpR promoter
     rep_origin      6932..7372
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacgcttac aatttacgcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccctgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      661 gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
      721 agtgtgctgg aattcggctt ggggatatcc accatggaga cagacacact cctgctatgg
      781 gtactgctgc tctgggttcc aggttccact ggtgacacaa gtttgtacaa aaaagttggc
      841 accatgacct tgcacaataa cagtacaacc tcgcctttgt ttccaaacat cagctcctcc
      901 tggatacaca gcccctccga tgcagggctg cccccgggaa ccgtcactca tttcggcagc
      961 tacaatgttt ctcgagcagc tggcaatttc tcctctccag acggtaccac cgatgaccct
     1021 ctgggaggtc ataccgtctg gcaagtggtc ttcatcgctt tcttaacggg catcctggcc
     1081 ttggtgacca tcatcggcaa catcctggta attgtgtcat ttaaggtcaa caagcagctg
     1141 aagacggtca acaactactt cctcttaagc ctggcctgtg ccgatctgat tatcggggtc
     1201 atttcaatga atctgtttac gacctacatc atcatgaatc gatgggcctt agggaacttg
     1261 gcctgtgacc tctggcttgc cattgactac gtagccagca atgcctctgt tatgaatctt
     1321 ctggtcatca gctttgacag atacttttcc atcacgaggc cgctcacgta ccgagccaaa
     1381 cgaacaacaa agagagccgg tgtgatgatc ggtctggctt gggtcatctc ctttgtcctt
     1441 tgggctcctg ccatcttgtt ctggcaatac tttgttggaa agagaactgt gcctccggga
     1501 gagtgcttca ttcagttcct cagtgagccc accattactt ttggcacagc catcgctgct
     1561 ttttatatgc ctgtcaccat tatgactatt ttatactgga gggtctttca ggaggccaaa
     1621 aggcagctcc agaagattga caaatctgag ggccgcttcc atgtccagaa ccttagccag
     1681 gtggagcagg gaggaaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     1741 aacttccaca tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     1801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     1861 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     1981 agcatggtga gaaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2041 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     2101 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2161 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2221 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     2281 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2341 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2401 cacaagctgg agtacaacgc tcctagcgtc gcagatgggc ggacggggca tggactccgc
     2461 agatcttcca agttctgctt gaaggagcac aaagccctca agacgctcag tgcgatcttg
     2521 cttgccttca tcatcacttg gaccccatac aacatcatgg ttctggtgaa caccttttgt
     2581 gacagctgca tacccaaaac cttttggaat ctgggctact ggctgtgcta catcaacagc
     2641 accgtgaacc ccgtgtgcta tgctctgtgc aacaaaacat tcagaaccac tttcaagatg
     2701 ctgctgctgt gccagtgtga caaaaaaaag aggcgcaagc agcagtacca gcagagacag
     2761 tcggtcattt ttcacaagcg cgcacccgag caggccttgt aagtcgacga acaaaaactc
     2821 atctcagaag aggatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca
     2881 cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc
     2941 atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgtta ggcggccgct
     3001 cgagatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3061 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3181 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3241 cttctgaggc ggaaagaacc agtggcggta atacggttat ccacagaatc gggggataac
     3301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3661 ttatccggta actatcgtct tgagtccaac ccggtaagac gcgacttatc gccactggca
     3721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3781 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4081 tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc
     4141 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa
     4201 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc
     4261 cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc
     4321 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
     4381 gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     4441 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     4501 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     4561 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     4621 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     4681 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     4741 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     4801 gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg
     4861 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     4921 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     4981 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     5041 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     5101 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     5161 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     5221 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     5281 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     5341 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg
     5401 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccggg
     5461 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     5521 tgggcggagt taggggcgga atgggcggag ttaggggcgg gactatggtt gctgactaat
     5581 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     5641 ttgctgacta attgaggagt taggggcgga atgggcggag ttaggggcgg gactatggtt
     5701 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     5761 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     5821 cctggggact ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag
     5881 gactcttcct ttttcaataa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5941 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6001 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6061 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6121 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6181 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6241 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6301 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6361 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6421 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6481 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6541 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6601 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6661 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6721 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6781 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6841 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6901 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     6961 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7141 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7261 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat at
//
