LOCUS       40924_14705        7474 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pDhtSK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Feng J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen 
            University, Department of Dermatology, 107 West Yanjiang Rd, 
            Guangzhou, Guangdong 510120, China
REFERENCE   2  (bases 1 to 7474)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen University,
            Department of Dermatology, 107 West Yanjiang Rd, Guangzhou, 
            Guangdong 510120, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..7474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(77..251)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    552..573
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        588..618
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    626..642
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     650..666
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        687..705
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    718..825
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(834..852)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(862..878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1171..1195
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2495..3121
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3558..4622
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4691..4885
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5229..5369
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5555..6143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6233..7024)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7449..7473
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
       61 ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg aattagaaat
      121 tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa
      181 cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta
      241 ttatggagaa actcgattgg cagctgctct agcatacgca aaccgctctc ccgcgcgttg
      301 gccgattcat tatgcagcct tcatgtcttt cctgcgttat cccctgattc tgtggataac
      361 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      421 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      481 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      541 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      601 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      661 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      721 ctccaccgcg gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat
      781 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag
      841 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
     1021 attgtaagcg ttaatgttct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1081 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     1141 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     1201 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     1261 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     1321 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     1381 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     1441 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     1501 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     1561 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     1621 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     1681 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     1741 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     1801 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     1861 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     1921 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     1981 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     2041 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     2101 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     2161 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     2221 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     2281 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     2341 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     2401 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     2461 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     2521 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     2581 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     2641 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     2701 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     2761 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     2821 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     2881 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     2941 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     3001 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     3061 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     3121 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     3181 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     3241 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     3301 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     3361 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     3421 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     3481 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     3541 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     3601 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     3661 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     3721 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     3781 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     3841 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     3901 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     3961 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     4021 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     4081 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     4141 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     4201 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     4261 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     4321 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     4381 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     4441 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     4501 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     4561 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     4621 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     4681 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     4741 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     4801 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     4861 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     4921 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     4981 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     5041 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     5101 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5161 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5221 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     5281 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5341 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     5401 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5461 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5581 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5641 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5701 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5761 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5821 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5881 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5941 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6001 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6061 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6181 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     6241 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     6301 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     6361 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     6421 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     6481 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     6541 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     6601 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     6661 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     6721 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     6781 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     6841 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     6901 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     6961 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     7021 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     7081 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     7141 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     7201 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     7261 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     7321 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     7381 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     7441 ctggctggtg gcaggatata ttgtggtgta aaca
//
